This study investigated the effects of high-frequency initial pulses on the efficiency (=total work output/high-energy phosphate consumption) of rat fast skeletal muscle. In situ rat medial gastrocnemius muscles performed 15 repeated shortening contractions (2 s(−1); velocity 50 mm s(−1)) with occluded blood flow while activated with triplets of 400 Hz followed by 60 Hz trains (T400;60) or with constant-frequency trains of either 60 or 91 Hz. All stimulation patterns consisted of six pulses. After the last contraction, the muscles were quickly freeze-clamped and analysed for metabolite levels. The calculated efficiencies were 20.4+/−3.0 mJ micromol(−1)P (N=7), 19.4+/−1.8 mJ micromol(−1)P (N=8) and 19.6+/−2.5 mJ micromol(−1)P (N=7; means +/− s.d.) for T400;60, 60 and 91 Hz stimulation respectively (P>0.05). It is concluded that, although high-frequency initial pulses can enhance muscle performance, the efficiency of rat fast skeletal muscle did not differ from that for submaximal constant-frequency stimulation patterns.

Abbate
F.
,
Sargeant
A. J.
,
Verdijk
P. W. L.
,
de Haan
A.
(
2000
).
The effects of high frequency initial pulses and post tetanic potentiation on the power output of skeletal muscle.
J. Appl. Physiol
88
,
35
–.
Baker
A. J.
,
Kostov
K. G.
,
Miller
R. G.
,
Weiner
M. W.
(
1993
).
Slow force recovery after long duration exercise: metabolic and activation factors in muscle fatigue.
J. Appl. Physiol
74
,
2294
–.
Bigland-Ritchie
B.
,
Jones
D. A.
,
Woods
J. J.
(
1979
).
Excitation frequency and muscle fatigue: electrical responses during human voluntary and stimulated contractions.
Exp. Neurol
64
,
414
–.
Binder-Macleod
S. A.
,
Barker
C. B.
III
(
1991
).
Use of a catchlike property of human skeletal muscle to reduce fatigue.
Muscle& Nerve
14
,
850
–.
Burke
R. E.
,
Radomin
P.
,
Zajac
F. E.
(
1970
).
Catch property in single mammalian motor units.
Science
168
,
122
–.
de Haan
A.
(
1998
).
The influence of stimulation frequency onforce—velocity characteristics of in situ rat medial gastrocnemius muscle.
Exp. Physiol
83
,
77
–.
de Haan
A.
,
de Jong
J.
,
van Doorn
J. E.
,
Huijing
P. A.
,
Woittiez
R. D.
,
Westra
H. G.
(
1986
).
Muscle economy of isometric contractions as a function of stimulation time and relative muscle length.
Pflugers Arch
407
,
445
–.
de Haan
A.
,
de Ruiter
C. J.
,
Lind
A.
,
Sargeant
A. J.
(
1993
).
Age-related changes in force and efficiency in rat skeletal muscle.
Acta Physiol. Scand
147
,
347
–.
de Haan
A.
,
Jones
D. A.
,
Sargeant
A. J.
(
1989
).
Changes in velocity of shortening, power output and relaxation rate during fatigue of rat medial gastrocnemius muscle.
Pflugers Arch
413
,
422
–.
de Haan
A.
,
Koudijs
J. C. M.
,
Verburg
E.
(
1996
).
Absence of an effect of fatigue on muscle efficiency during high-intensityexercise in rat skeletal muscle.
Eur. J. Appl. Physiol
72
,
570
–.
Gorassini
M.
,
Bennett
D. J.
,
Kiehn
O.
,
Eken
T.
,
Hultborn
H.
(
1999
).
Activation patterns of hindlimb motor units in the awake rat and their relation to motoneuron intrinsic properties.
J. Neurophysiol
82
,
709
–.
Henneman
E.
,
Clamann
H. P.
,
Gillies
J. D.
,
Skinner
R. D.
(
1974
).
Rank order of motoneurons within a pool, law of combination.
J. Neurophysiol
37
,
1338
–.
Hennig
R.
,
Lømo
T.
(
1985
).
Firing patterns of motor units normal rats.
Nature
314
,
164
–.
Jones
D. A.
,
Bigland-Ritchie
B.
,
Woods
J. J.
(
1979
).
Excitation frequency and muscle fatigue: mechanical responses during voluntary and stimulated contractions.
Exp. Neurol
64
,
401
–.
Miller
R. G.
,
Kent-Braun
J. A.
,
Sharma
K. R.
,
Weiner
M. W.
(
1995
).
Mechanisms of human muscle fatigue; quantitating the contribution of metabolic factors and activation impairment.
In. Adv. Exp. Med. Biol
384
,
195
–.
Sandercock
T. G.
,
Heckman
C. J.
(
1997
).
Doublet potentiation during eccentric and concentric contractions of cat soleus muscle.
J. Appl. Physiol
82
,
1219
–.
Westerblad
H.
,
Allen
D. G.
(
1992
).
Myoplasmic Mg2+concentration in Xenopus muscle fibres at rest, during fatigue and during metabolic blockade.
Exp. Physiol
77
,
733
–.
Zajac
F. E.
,
Young
J. L.
(
1980
).
Properties of stimulus trains producing maximum tension—time area per pulse from single motor units in medial gastrocnemius muscle of the cat.
J. Neurophysiol
43
,
1206
–.
This content is only available via PDF.