Acetate oxidation was examined by (13)C nuclear magnetic resonance in skeletal muscle from adult and old rats. Rats fasted for 5 days were perfused with [2-(13)C]acetate over 2 h, and muscle extracts were analyzed for [(13)C]glutamate isotopomers. This study shows that approximately 80 % of acetyl-coenzyme A entering the tricarboxylic cycle was derived from substrate infusion in both adult and old rats, and that the flux through anaplerotic pathways was approximately 21 % of the flux through citrate synthase. These data demonstrate that skeletal muscle from adult and old rats oxidizes the same proportion of exogenous acetate.
Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: a (13)C NMR study
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V. Mezzarobba, G. Bielicki, F.M. Jeffrey, M. Mignon, J.P. Renou, J. Grizard, D. Meynial-Denis; Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: a (13)C NMR study. J Exp Biol 15 March 2000; 203 (6): 995–1001. doi: https://doi.org/10.1242/jeb.203.6.995
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