Comparative physiologists and biochemists working with tissues at varying temperatures and ionic strength are required to adjust apparent equilibrium constants (K') of biochemical reactions to the experimental conditions prior to calculating cytosolic bioenergetic parameters (transformed Gibbs free energy of formation, DeltafG' ATP; cytosolic phosphorylation ratio, [ATP]/[ADP][Pi]; [phosphocreatine]: [orthophosphate] ratio [PCr]/[Pi]) and kinetic parameters (free [ADP], [Pi] and [AMP]). The present study shows how to adjust both K' and the equilibrium constants of reference reactions (Kref) of creatine kinase (ATP: creatine N-phosphotransferase; EC 2.7.3.2), adenylate kinase (ATP:AMP phosphotransferase; EC 2.7.4.3) and adenosinetriphosphatase (ATP phosphohydrolase; EC 3.6.1.3) to temperature and ionic strength. This information, together with our previous study showing how to adjust equilibria to varying pH and pMg, is vital for the quantification of organ and tissue bioenergetics of ectotherms and endotherms under physiological conditions.
In the field: an interview with Harald Wolf
(update)-Conversation.jpg?versionId=3772)
In our new Conversation, Harald Wolf talks about his fieldwork experiences working with desert ants in Tunisia to understand their navigation.
Propose a new Workshop
-GSWorkshop.png?versionId=3772)
Our Workshops bring together leading experts and early-career researchers from a range of scientific backgrounds. Applications are now open to propose Workshops for 2024, one of which will be held in a Global South country.
Julian Dow steps down and John Terblanche joins the JEB team
-NewEditor.png?versionId=3772)
After 15 years with the journal, Julian Dow from University of Glasgow, UK, is stepping down as a Monitoring Editor. We wish Julian all the best for the future and welcome John Terblanche, Stellenbosch University, South Africa, who is joining the team. Julian talks about his long association with The Company of Biologists and the journal and John tells us about his life and career in this News article.
An accelerometer-derived ballistocardiogram method for detecting heart rate in free-ranging marine mammals
-Whales.jpg?versionId=3772)
Max Czapanskiy and co show how the resting heart rates of blue whales are immortalized in the accelerometry traces collected by motion sensing data tags.
Global change and physiological challenges for Amazonian fish
-Review.png?versionId=3772)
In their Review, Adalberto Luis Val and Chris Wood discuss the physiological threats to the unique and diverse fish fauna of Amazonia.
Social media
