Although previous investigations have shown that experimental increases and decreases of the concentration of extracellular Ca2+ produce correlated changes in the stiffness of holothurian dermis, they have failed to determine whether the Ca2+-correlated changes were due to Ca2+-dependent cellular events or to direct effects of Ca2+ on the viscosity of the extracellular matrix. We have addressed this question by testing two explicit predictions of the latter hypothesis: that dermal stiffness should be correlated with the Ca2+ concentration in the absence of viable cells; and that, in the presence of a normal extracellular Ca2+ concentration, drugs that inhibit cellular pathways dependent on Ca2+ should not affect dermal stiffness. Our results are inconsistent with the hypothesis and support the alternative hypothesis that Ca2+ is important only in the cellular regulation of dermal stiffness. In addition, we have extracted from dermal cells an organic factor that stiffens the extracellular matrix.
Evidence that calcium-dependent cellular processes are involved in the stiffening response of holothurian dermis and that dermal cells contain an organic stiffening factor
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J Trotter, T Koob; Evidence that calcium-dependent cellular processes are involved in the stiffening response of holothurian dermis and that dermal cells contain an organic stiffening factor. J Exp Biol 1 September 1995; 198 (9): 1951–1961. doi: https://doi.org/10.1242/jeb.198.9.1951
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