The mechanism that underlies transcellular Ca2+ reabsorption in the kidney of the euryhaline teleost Oreochromis mossambicus was studied. Preparations of membrane vesicles made from the kidneys of freshwater- and seawater-adapted fish were more than sevenfold enriched in the basolateral plasma membrane marker Na+/K+-ATPase. Significant recovery of NADH­ cytochrome c reductase enzyme activity and of oxalate-stimulated Ca2+ pump activities in the membrane preparations indicated that the membrane fraction was of endoplasmic reticular origin. Indeed, thapsigargin specifically inhibited Ca2+ pump activity that could be attributed to oxalate-permeable endoplasmic reticular fragments. Kinetic analysis of thapsigargin-insensitive Ca2+ pump activity indicated the existence of a homogeneous, high-affinity, ATP-driven Ca2+ pump. No Na+-driven Ca2+ transport mechanism could be demonstrated. Plasma membrane Ca2+ pump activity was 56 % lower in preparations from seawater-adapted fish than in preparations from freshwater-adapted fish, suggesting a physiological role for this Ca2+ pump activity in renal Ca2+ handling by euryhaline species, with an involvement in the regulation of Ca2+ reabsorption.
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JOURNAL ARTICLE| 01 June 1995
Calcium pump activities in the kidneys of Oreochromis mossambicus
Online Issn: 1477-9145
Print Issn: 0022-0949
J Exp Biol (1995) 198 (6): 1351–1357.
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M Bijvelds, A Heijden, G Flik, P Verbost, Z Kolar, S Bonga; Calcium pump activities in the kidneys of Oreochromis mossambicus. J Exp Biol 1 June 1995; 198 (6): 1351–1357. doi: https://doi.org/10.1242/jeb.198.6.1351
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