The present study examines the importance of the Na+ current(INa) in the excitability of atrial and ventricular myocardium of the rainbow trout heart. Whole-cell patch-clamp under reduced sarcolemmal Na+ gradient showed that the density of INa is similar in atrial and ventricular myocytes of the trout heart, and the same result was obtained when INa was elicited by chamber-specific action potentials (AP) in normal physiological saline solution. However, the maximum rate (Vmax) of AP upstroke, measured with microelectrodes in intact trout heart, was 21% larger in atrium than ventricle, and thus in variance with the similar INa density of the two myocyte types. Furthermore, Vmax calculated from the INa was 2.1 and 3.2 times larger for atrium and ventricle, respectively, than the values obtained from the APs. The discrepancy between INa of isolated myocytes and Vmax of intact muscle is only partly explained by the inward rectifier K+ current(IK1), which overlaps INa and decreases the net depolarising current. Clear differences exist in the voltage dependence of steady-state activation and inactivation as well as in the inactivation kinetics of INa between atrial and ventricular myocytes. As a result of a more negative voltage dependence of INa activation, smaller IK1 and higher input resistance of atrial myocytes, the voltage threshold for AP generation is more negative in atrium than ventricle of the trout heart. These findings suggest that atrial muscle is more readily excitable than ventricular muscle,and this difference is partly due to the properties of the atrial INa.
The generation of action potentials (AP) and synchronized spread of excitation are essential features for the coordinated pumping action of the heart. The spontaneous rhythm of the heart is generated by pacemaker cells that produce slow APs which excite atrial myocytes to produce fast but short-duration APs (Irisawa,1978; Bouman and Jongsma,1986; Golod et al.,1998). The atrial muscle excitation wave enters the ventricular myocardium, where it produces even quicker rising and more prolonged APs. This sequence of events requires a particular composition of membrane currents from each myocyte type to enable adequate excitability for each cardiac compartment and tuning of excitability and impulse conduction to the unique contractile properties of atrial and ventricular myocardium(Hume and Uehara, 1985; Schram et al., 2002; Marionneau et al., 2005).
The first current to be activated in atrial and ventricular myocytes is the fast Na+ current (INa), which provides the necessary charge to depolarize the cell membrane and activate other ion channels in the production of chamber-specific APs(Schram et al., 2002; Kleber and Rudy, 2004). Although the properties of INa are thought to primarily determine the excitability of myocytes and conduction velocity of the cardiac AP, the ability of INa to depolarize the membrane is also dependent on the K+ currents and other repolarising currents that are activated in the voltage range of AP onset(Golod et al., 1998). In this regard, the time-independent inward rectifier K+ current(IK1) is particularly important since it generates outward K+ flux immediately when membrane potential exceeds the reversal potential of K+ ions. Previous studies have shown that there are dramatic differences in the density of the IK1 between atrial and ventricular myocytes of the trout heart(Vornanen et al., 2002) that might set differential demands on INa in regulating excitability of the two cardiac chambers. This prompted us to compare the properties of INa in atrial and ventricular myocytes of the rainbow trout heart to identify the relative role of chamber-specific INa in the depolarisation of the fish heart. In addition to Na+ and K+ currents, factors that may not necessarily be inherent to isolated myocytes, such as intercellular electric coupling between myocytes and nonmyocyte cells, are likely to affect AP generation(Camelliti et al., 2005). Therefore, the rate of AP upstroke of intact atrium and ventricle were compared to the theoretical values obtained from the density of INa in isolated myocytes.
Materials and methods
Rainbow trout (Oncorhynchus mykiss Walbaum) were obtained from a local fish farm near the University of Joensuu (Finland). In the lab, fish(257.16±12.13 g, N=27) were reared in temperature-controlled 1000 litre stainless steel tanks with a continuous supply of aerated groundwater at the rate of 0.5 l min-1. Fish were held for at least 4 weeks at constant temperature (4°C) under a 15 h:9 h light:dark photoperiod before experimentation. During that time, trout were fed five times per week ad libitum with commercial fish fodder (Biomar,Brande, Denmark).
Atrial and ventricular myocytes were enzymatically isolated using previously published methods (Vornanen,1997). Briefly, fish were stunned with a blow to the head, the spine was cut and the heart was excised. A metallic cannula was advanced through the bulbus arteriosus into the ventricle, and the heart was retrogradely perfused first with a nominally Ca2+-free,low-Na+ solution (containing in mmol l-1: 100 NaCl, 10 KCl, 1.2 KH2PO4, 4 MgSO4, 50 taurine, 20 glucose and 10 Hepes at pH 6.9 at 20°C) for 10 min and then with a fresh low-Na+ solution supplemented with 0.75 mg ml-1collagenase (Type IA, Sigma, St Louis, MO, USA), 0.5 mg ml-1trypsin (Type IX, Sigma) and 0.5 mg ml-1 fatty-acid-free bovine serum albumin for 15 min from a height of 50 cm. Both solutions were oxygenated with 100% O2, and the enzyme solution was recycled using a peristaltic pump. After enzymatic digestion, atrium and ventricle were excised, placed in fresh low-Na+ solution in a Petri dish and cut into small pieces with scissors. Single cells were released by agitating tissue pieces through the opening of a Pasteur pipette. Myocytes were stored at 6°C and used within 8 h of isolation. All experiments were performed with the consent of the local committee for animal experimentation.
Whole-cell patch-clamp experiments
A small sample of myocyte suspension was transferred to a recording chamber(RC-26; Warner Instrument Corp., Hamden, CT, USA; volume 150 μl) and cells were allowed to settle on the chamber bottom before superfusing with external saline solutions at a rate of 1.5-2.0 ml min-1. First, the myocytes were perfused with normal K+-based saline (containing in mmol l-1: 150 NaCl, 5.4 KCl, 1.8 CaCl2, 1.2 MgCl2,10 glucose, 10 Hepes, 0.01 nifedipine, pH adjusted to 7.7 with NaOH), where gigaohm seal and whole-cell patch-clamp recording of the myocytes were established. Internal perfusion of the myocytes with pipette solution(containing in mmol l-1: 5 NaCl, 130 CsCl, 1 MgCl2, 5 EGTA, 5 Mg2ATP, 5 Hepes, pH adjusted to 7.2 with CsOH) continued for at least 3 min in order to allow buffering of intracellular Ca2+ with 5 mmol l-1 EGTA. Then, solution flow could be switched to a low-Na+ external solution (containing in mmol l-1: 20 NaCl, 120 CsCl, 1 MgCl2, 0.5 CaCl2,10 glucose, 10 Hepes, 0.01 nifedipine, pH adjusted to 7.7 with CsOH) without inducing contracture in the patched myocyte. INa was recorded in the low-Na+ saline solution at 4°C(Haverinen and Vornanen,2004).
The whole-cell voltage-clamp measurements of INa were performed using an Axopatch 1-D amplifier with a CV-4 1/100 headstage (Axon Instruments, Union City, CA, USA). The digitised data were stored on the hard drive of the computer using the Clampex 8.2 software (Axon Instruments). The recordings were analysed off-line with Clampfit 8.2 and SigmaPlot 6.0 (SPSS,Inc., Chicago, IL, USA) software. Patch pipettes were pulled from borosilicate glass (Garner, Claremont, CA, USA) using a vertical two-stage puller(L/M-3P-A; List-Electronic, Darmstadt, Germany). Offset potentials were zeroed just before the formation of gigaohm seal, and the pipette capacitance(7.43±0.08 pF, N=108) was compensated for after the seal formation. The membrane was ruptured by a short voltage pulse (zap), and capacitive transients were eliminated by adjusting series resistance and cell capacitance compensation circuits. Mean resistance of the electrodes and total access resistance before compensation were 3.23±0.06 and 9.88±0.12 MΩ (N=108), respectively. INa was elicited from the holding potential of -120 mV with different pulse protocols and recorded at a sampling rate of 10 kHz. The recordings were low-pass filtered at 5 kHz. The calculated liquid-junction potential of the electrodes was about 1.5 mV, which was not corrected in the results.
Low external Na+ concentration (20 mmol l-1), low experimental temperature (4°C) and relatively small size of the myocytes(51.08±1.45 and 53.50±1.95 pF for atrial and ventricular myocytes, respectively) kept the size of INa small (<2 nA) and allowed adequate voltage control of the current (maximally a 2-mV error with 10 MΩ access resistance). To ensure good voltage control, a minimum of 80% series resistance compensation was routinely applied.
Steady-state activation and inactivation of INa
where GNa is the Na+ conductance of the membrane, INa is the peak Na+ current at a given membrane potential (V) and Vrev is the reversal potential of INa.
Inactivation of INa
The development of rested-state inactivation was examined by a protocol that consisted of a conditioning prepulse from -120 to -80 mV with variable(30-360 ms) duration, followed by a short return (3 ms) to the holding potential and a test pulse to -20 mV for 30 ms. The peak INa elicited by test pulses was plotted as a function of the prepulse duration and fit to a single exponential function to obtain the time constant for the development of rested-state inactivation. Time-dependent recovery of INa from inactivation was examined using a pairedpulse protocol where two successive 100 ms pulses from -120 to -20 mV were separated by a variable (40-400 ms) delay at -82 mV. The peak INa during the latter pulse was plotted as a function of time and fit to a single exponential function(y=y0+a-bt) to obtain the time constant (τ=1/-b) of recovery from inactivation.
The time constants of INa inactivation kinetics were derived by fitting the decay phase of the INa at different membrane potentials (-40 to +10 mV) with a single exponential equation using the Chebyshev transformation procedure of the Clampfit software package. The kinetics of INa activation was assessed by determining the time from the start of voltage-clamp pulse to the peak inward current at -40 to +10 mV.
Voltage threshold of the net inward current
The depolarising power of INa, in the presence of all ion currents of the cardiac myocyte, was determined by measuring the threshold voltage for net inward current in normal physiological saline (see recording of APs) in the absence of channel blockers. Pipette solution in these experiments contained (in mmol l-1): 140 KCl, 5 Na2ATP,1 MgCl2, 0.03 Tris-GTP, 10 Hepes and pH adjusted to 7.2 with KOH. Currents were elicited from the resting membrane potential of -82 mV with 30 ms depolarising pulses at 2 mV increments. Membrane time constant (τ) and series resistance of atrial and ventricular myocytes were determined from small subthreshold depolarisations. Input resistance (MΩ) of atrial and ventricular cells was calculated by using the equation R=τ/cell size, where τ is in s and cell size is in pF.
Recording of action potentials
Atrial and ventricular APs were recorded from multicellular preparations. The whole heart was excised, and the ventricle was cut into two parts to allow free access of oxygenated (100% O2) solution to the tissue. The heart was fixed with insect pins on the Sylgaard™-coated bottom of the 15-ml recording chamber filled with physiological saline (in mmol l-1): 150 NaCl, 3 KCl, 1.2 MgSO4, 1.2 NaH2PO4, 1.8 CaCl2, 10 Hepes and 10 glucose adjusted to pH 7.7 with NaOH. The spontaneously beating heart was allowed to equilibrate at 4°C for about 1 h to reach a stable heart rate(31.2±1.2 beats min-1). APs were recorded with sharp microelectrodes filled with 3 mol l-1 KCl. Analogue signals were amplified by a high-impedance amplifier (KS-700; WPI, Sarasota, FL, USA) and digitized (Digidata-1200 AD/DA board; Axon Instruments) with a sampling rate of 2 kHz before storing on the computer with the aid of Axotape (Axon Instruments Inc., Union City, CA, USA) acquisition software. The maximum rate of AP upstroke (Vmax) was obtained by differentiation of the voltage signal in SigmaPlot. The Vmax measured in intact tissue was compared to the theoretical value Vmaxobtained from the peak INa under AP clamp according to the relationship between membrane voltage (Vm), membrane capacitance (Cm) and membrane current:dVm/dt=-INa/Cm. The value of specific membrane capacitance was taken to be 1.59 pF mm-2 (Vornanen,1997).
Mean values between atrial and ventricular myocytes and between controls and treatments were compared with Student's t-test for unpaired data. P values of <0.05 were regarded as statistically significant. Data are presented as means ± s.e.m.
Characteristics of atrial and ventricular action potentials
Action potentials recorded with sharp microelectrodes from intact atrium and ventricle (at 4°C) are strikingly different. Ventricular AP is approximately double in duration and has a smaller amplitude and slightly less negative resting membrane potential (RMP) in comparison with atrial AP(Fig. 1A;Table 1). In both cardiac compartments, the Vmax occurred at the membrane potential of -20 mV, but the absolute rate was 21% faster in atrium than ventricle (P<0.05) (Fig. 1B), suggesting differences in net depolarising current.
Size and voltage-dependence of INa
The current-voltage relationships of INa in atrial and ventricular myocytes are shown in Fig. 2A. INa activated near -70 mV, peaked at about-20 mV and reversed close to the theoretical reversal potential (32 mV) of INa. At negative voltages, atrial INawas significantly larger than ventricular INa. Furthermore, the half-voltages (V0.5) of both steady-state activation and inactivation were about 6 mV more negative in atrial than ventricle myocytes (Fig. 2B; Table 2). However, the peak density of INa was similar in both myocyte types and thus cannot explain the chamber-specific differences in the rate of AP upstroke.
Inactivation of INa
In order to clarify whether differences in the time domain of INa could be responsible for differences in Vmax, we examined the inactivation of INa. The rate of transfer of Na+ channels from resting closed state to the inactivated closed state was measured by clamping the membrane potential from -120 to -80 mV for different durations and then recording the INa at -20 mV. Since the opening of Na+ channels is unlikely at -80 mV(Fig. 1A), the decrease in amplitude of INa as a function of prepulse duration is most likely due to the direct transfer of Na+ channels from resting closed state to inactivated closed state without intervening opening. At -80 mV, the development of rested-state inactivation of INawas faster and more extensive in atrial than ventricular myocytes in accordance with the availability curves(Fig. 3A). In contrast to the development of the rested-state inactivation, the time constant of recovery from INa inactivation at -82 mV was similar in ventricular and atrial myocytes (Fig. 3B; Table 2).
Inactivation kinetics was examined in a voltage range from -40 to +10 mV. At 0 and +10 mV, where all Na+ channels are activated, the kinetics of inactivation was faster in ventricular than atrial myocytes(Fig. 4). That the differences were not seen at other voltages is likely due to the 6 mVdifference in the voltage position of steady-state activation curve, which might obscure the faster inactivation of ventricular INa at more negative voltages. No differences were found in activation kinetics.
INa under action potential clamp
As there were differences in RMP, shape of AP, inactivation kinetics and voltage dependence of INa between atrium and ventricle, INa was next studied under more physiologically relevant conditions. INa was elicited at physiological external Na+ concentration with chamber-specific APs, which were delivered at the physiological beating frequency of 0.3 Hz. The peak density of INa and the maximum rate of membrane depolarization calculated from INa were not significantly different between atrial and ventricular myocytes(Fig. 5). These findings indicate that the size of INa under physiologically realistic conditions does not differ between atrium and ventricle and cannot therefore be responsible for the differences in Vmax found in intact atrium and ventricle. Interestingly, the Vmaxcalculated from the peak INa was 2.1 and 3.2 times larger,for atrium and ventricle, respectively, than the Vmaxmeasured in the intact tissue, suggesting the presence of repolarising current(s) and/or other effects that antagonize INain situ (Fig. 5).
Voltage threshold for the net inward current
To be able to elicit an AP, the amplitude of INa must exceed the conductance of simultaneously activated outward currents. In order to determine the voltage threshold for the net inward current, small depolarising pulses were delivered with 2 mV increments to the cells from the resting membrane potential (-82 mV) in the presence of normal physiological levels of external Na+, K+ and Ca2+. The net outward current between -70 and -50 mV was bigger and the voltage threshold for the net inward current more positive in ventricular versus atrial cells (Fig. 6; Table 2), suggesting that differences in repolarising currents might contribute to the differences in AP generation between atrium and ventricle. Furthermore, the input resistance of the atrial myocytes was almost an order of magnitude larger than that of the ventricular myocytes.
Voltage-gated Na+ channels determine the rate and extent of the AP upstroke, which are important in the control of impulse conduction velocity and in the maintenance of appropriate waves of excitation through different compartments of the working heart (Fozzard,1977). Atrial and ventricular muscle have specialized functions for cardiac pumping and therefore have different contractile and electrical properties, which are likely to set chamber-specific demands on the Na+ channels. The aim of this study was to analyze putative atrio-ventricular differences in the function of Na+ channels and relate them to the functional heterogeneity of atrial and ventricular tissue in the whole heart. Indeed, the present results indicate that the properties of INa are different in atrial and ventricular myocytes of the trout heart, similar to what has recently been described for mammals(Li et al., 2002). Thus, in general, INa heterogeneity may be necessary to satisfy the demands of electrical excitability in the functionally specialized compartments of the vertebrate heart.
INa and the rate of AP upstroke
INa is the largest inward current in cardiac myocytes and therefore a prime determinant for the rate of AP upstroke and impulse propagation. Vmax in the trout heart (16-20 V s-1 at 4°C) was similar to values previously measured in frog ventricular myocytes (26.4 V s-1 at 15°C; Seyama and Yamaoka, 1988) and in skate (Dasuyatis akajei) heart (9.5 V s-1 at 20°C; Seyama and Irisawa, 1967) at low temperatures but more than an order of magnitude smaller than in mammalian heart (270 V s-1) at 35°C(Kiyosue et al., 1993). The large difference in Vmax between mammalian and ectothermic hearts is mostly explained by temperature differences.
The Vmax was ∼20% faster in atrial tissue than ventricular tissue from the trout heart. This difference is not, however,readily explained by the properties of INa, as the peak density of INa was similar in atrial and ventricular myocytes. INa is the first depolarising current activated in membrane excitation and elicits all or no AP when its amplitude exceeds the amplitude of simultaneously activated repolarising currents. Thus, the lower Vmax in ventricle might be due to the presence of large repolarising currents that antagonize INa. In trout cardiac myocytes, there are two major K+ currents, the rapid delayed rectifier current, IKr, and the background inward rectifier K+ current, IK1(Vornanen et al., 2002). The delayed rectifier is a relatively slow, time-dependent current and does not activate to any significant degree during the rapid upstroke of the AP and therefore cannot antagonize INa. As a time-independent current, IK1 immediately generates an outward surge of current that overlaps INa when the driving force for K+ efflux is restored by membrane depolarisation(Rasmusson et al., 1990). Previous studies have shown the conductance of IK1 in trout atrial myocytes at 10°C is less than 5% (0.009 nS pF-1)of its value in ventricular myocytes (0.198 nS pF-1) at the same temperature (Vornanen et al.,2002). Therefore, the greater IK1 of ventricular myocytes might explain in part the difference in Vmax between atrial and ventricular muscle of the trout heart. However, the maximum density of the outward K+ current is small (less than 10%) in comparison to INa, and the peak IK1 occurs earlier (around -60 mV) in the AP than the peak INa (-20 mV), suggesting that other factors in addition to IK1 might be contributing to the Vmaxdifference between atrium and ventricle.
Interestingly, the Vmax calculated from the density of INa in single myocytes was 2-3 times larger than the measured Vmax of the intact tissue. The difference between the measured and calculated values could be simply caused by the assumptions made for Vmax calculation. If intracellular[Na+] of the intact muscle were substantially higher than the pipette [Na+] (10 mmol l-1), then we could have overestimated physiological INa in the patch-clamp experiments. Since intracellular [Na+] of the vertebrate cardiac myocytes is between 4 and 16 mmol l-1 (usually around 10 mmol l-1; Bers et al.,2003) and doubling of the intracellular [Na+] from 10 to 20 mmol l-1 would reduce Na+ conductance only about 15%, this does not explain the difference. The other possible source of error is the value of specific membrane capacitance. Instead of the conventional 1 pF mm-2, we used the value of 1.59 pF mm-2 determined for fish cardiac myocytes (Vornanen,1997). However, the use of the higher capacitance value will decrease, not increase, the difference between measured and calculated Vmax.
In fact, the lower Vmax of intact tissue in comparison to isolated myocytes is an expected finding. Under patch-clamp conditions, the cardiac myocyte is an `ideally' space-clamped cell, where INa is solely used to change the charge on the membrane capacitance of that particular cell. In multicellular tissue, myocytes are resistively coupled not only to other myocytes but also to cardiac fibroblasts that function as current sinks (Camelliti et al., 2005). INa of the activated myocyte is thus divided between discharging the local membrane capacitance and depolarising the membrane of resistively coupled cells via axial current flow (Kleber and Rudy,2004). Thus, the substantially larger difference between measured and calculated Vmax in ventricle in comparison to atrium suggests that resistive coupling with myocyte and nonmyocyte cells might be more extensive in ventricular than atrial myocardium and thus might contribute to lower Vmax of the trout ventricle.
INa and excitability
Cardiac myocytes are electrically coupled and function both as source and sink for current flow and will therefore affect each other's electrical activity. Apart from its significance in impulse propagation, the properties of INa affect how different cardiomyocytes interact with each other to guarantee orderly generation and spread of excitation throughout the heart. In this respect, interaction of atrial myocytes with pacemaker cells of the sinus venosus, and interaction of ventricular myocytes with myocytes of the atrioventricular canal, is crucial for function of the fish heart (Arbel et al., 1977; Irisawa,1978; Sedmera et al.,2003). The excitability of atrial myocyte should be high, so that pacemaker cells are able to securely elicit atrial excitation without any danger of becoming strongly influenced by atrial APs, while excitability of ventricular myocytes might be lower to prevent accidental arrhythmic firing by spontaneous ectopic foci (Joyner et al.,1998). Indeed, the lower voltage threshold for the net inward current of atrial myocytes suggests that they are more readily excited than ventricular myocytes. The low threshold value for AP generation of the atrial myocytes is a function of both a more negative activation threshold of the INa and a smaller K+ outward current in the voltage range of the AP threshold in comparison to ventricular myocytes. Furthermore, the very high input resistance of trout atrial myocytes (due to the small IK1) improves atrial excitability.
Taken together, the present results show that voltage dependence of INa is more negative in atrial than ventricular myocytes,but maximum density of INa is similar in both cell types of the trout heart. As a consequence of more negative voltage dependence for INa activation, smaller IK1 and higher input impedance of atrial myocytes, the current required to trigger an AP is likely to be smaller in atrial than ventricular myocytes. This makes atrial myocytes readily excitable by pacemaker cells of the sinus venosus. The larger Vmax of atrial muscle cannot be ascribed to the properties of atrial INa, and therefore other factors such as less extensive resistive coupling of atrial myocytes with other myocytes and non-muscle cells and smaller overlapping IK1 in comparison to ventricular muscle might be involved.
This study was supported by a grant from the Academy of Finland (projects No. 78045 and 210400) to M.V. Anita Kervinen is appreciated for skilful technical assistance.