Intracellular symbiosis requires that the host satisfy the symbiont's metabolic requirements, including the elimination of waste products. The hydrothermal vent tubeworm Riftia pachyptila and the hydrocarbon seep worm Lamellibrachia cf luymesi are symbiotic with chemolithoautotrophic bacteria that produce sulfate and protons as end-products. In this report, we examine the relationship between symbiont metabolism and host proton equivalent elimination in R. pachyptilaand L. cf luymesi, and the effects of sulfide exposure on proton-equivalent elimination by Urechis caupo, an echiuran worm that lacks intracellular symbionts (for brevity, we will hereafter refer to proton-equivalent elimination as `proton elimination'). Proton elimination by R. pachyptila and L. cf luymesi constitutes the worms' largest mass-specific metabolite flux, and R. pachyptilaproton elimination is, to our knowledge, the most rapid reported for any metazoan. Proton elimination rates by R. pachyptila and luymesi correlated primarily with the rate of sulfide oxidation. Prolonged exposure to low environmental oxygen concentrations completely inhibited the majority of proton elimination by R. pachyptila,demonstrating that proton elimination does not result primarily from anaerobic metabolism. Large and rapid increases in environmental inorganic carbon concentrations led to short-lived proton elimination by R. pachyptila, as a result of the equilibration between internal and external inorganic carbon pools. U. caupo consistently exhibited proton elimination rates 5-20 times lower than those of L. cf luymesi and R. pachyptila upon exposure to sulfide. Treatment with specific ATPase inhibitors completely inhibited a fraction of proton elimination and sulfide and inorganic carbon uptake by R. pachyptila, suggesting that proton elimination occurs in large part via K+/H+-ATPases and Na+/H+-ATPases. In the light of these results, we suggest that protons are the primary waste product of the symbioses of R. pachyptila and L. cf luymesi, and that proton elimination is driven by symbiont metabolism, and may be the largest energetic cost incurred by the worms.

The discovery of chemoautotrophic symbionts in the hydrothermal vent tubeworm Riftia pachyptila expanded our concept of symbioses(Cavanaugh et al., 1981; Felbeck, 1981). In these associations, the bacteria fix inorganic carbon and oxidize reduced inorganic substrates, such as reduced sulfur compounds, to produce energy. Members of the class Vestimentifera, such as R. pachyptila and other tubeworms found at hydrothermal vents, hydrocarbon seeps and other chemically reduced deep-sea environments, exhibit a suite of morphological and biochemical adaptations to their association (Childress and Fisher, 1992). When mature, these worms do not possess a digestive tract. Instead, the symbionts are housed within host cells in a vascularized organ called the trophosome(Jones, 1981). The symbionts have no direct contact with the external milieu, and the host acquires all the metabolites required for chemoautotrophic metabolism.

Both vent and seep vestimentiferan symbionts are sulfide specialists that specifically utilize hydrogen sulfide to produce energy(Wilmot and Vetter, 1990). This metabolic process yields two primary end-products, an oxidized sulfur compound (such as thiosulfate or sulfate) and protons(Nelson and Hagen, 1995). Although a small proportion of these protons may be utilized in other microbial reductive metabolic processes, e.g. the reduction of inorganic carbon and nitrate (Girguis et al.,2000), the surplus protons must be eliminated from the symbionts and their host into the environment.

In a previous paper, we described extremely high rates of net proton elimination into the environment by the tubeworm R. pachyptila, the dominant tubeworm at the hydrothermal vent communities along the East Pacific Rise (Girguis and Childress,1998). However, we did not measure the quantitative relationship between proton elimination and host or symbiont metabolism. In the present study, we address these relationships, in particular the effects of sulfide,inorganic carbon and oxygen uptake on net proton elimination by the deep-sea vestimentiferan tubeworms R. pachyptila and Lamellibrachiacf luymesi (Kennicutt et al.,1985), and by the echiuran worm Urechis caupo. L. cf luymesi flourishes at the hydrocarbon seeps in the Gulf of Mexico(Macdonald et al., 1989). U. caupo is an echiuran worm that inhabits sulfide-rich environments and possesses mechanisms for oxidizing sulfide to prevent metabolic poisoning(Menon and Arp, 1998). Because U. caupo does not possess symbionts to which it is metabolically coupled, any observed proton elimination by U. caupo during sulfide exposure should be the result of sulfide detoxification. We chose to study U. caupo as a means of examining proton elimination that does not result from intracellular symbiont metabolism (note that vestimentiferans cannot survive without their symbionts, so they cannot be used for such experiments). In addition, we used four ATPase inhibitors to examine the mechanisms of proton elimination by R. pachyptila and L. cf luymesi would correlate primarily with symbiont metabolic processes and that any disruption to proton elimination (e.g. by the use of these inhibitors) would have negative repercussions on the metabolism of both the host and the symbiont.

Animal collection and maintenance

Riftia pachyptila Jones tubeworms were collected from hydrothermal vent sites along the East Pacific Rise (12°48′N, 103°56′W and 9°50′N, 104°18′W), at a depth of approximately 2600 m,during expeditions in April 1996 (HOT 96), November 1997 (HOT 97) and November 1998 (LARVE 98). Worms were collected daily by the DSV Alvin and brought to the surface in a thermally insulated container(Mickel and Childress, 1982). Upon arrival at the surface, the worms were immediately placed into flow-through, high-pressure respirometer aquaria(Girguis et al., 2000). During our HOT 97 experiment on the relationship between oxygen uptake and proton elimination, three tubeworms that had been collected 3 days earlier were kept in maintenance aquaria and later used for experimentation. The maintenance aquaria (Goffredi et al.,1997) are distinct from our respirometer aquaria, and are capable of sustaining 16-20 worms under in situ vent conditions(ΣCO2=5-6 mmoll-1, ΣH2S=250-600μmoll-1, [O2]=100-400 μmoll-1,[NO3-]=40 μmoll-1, pH 6.5, temperature 12°C, pressure 20.6 MPa; Σ is used to indicate the total concentrations of all ionic species of inorganic carbon or sulfide). In an earlier report, a comparison of freshly captured worms with maintenance worms showed no differences in ΣH2S or O2 uptake rates when measured in respirometer aquaria(Girguis et al., 2000).

A clump of Lamellibrachia cf luymesi (van de Land and Nørrevang) tubeworms were collected by the DSV Johnson Sea Link from the outskirts of the Brine Pool NR1 hydrocarbon seep site(27°43′24″N, 91°16′30″W) at a depth of approximately 650 m during an expedition to the Gulf of Mexico in July 1998. L. cf luymesi were brought to the surface in a temperature-insulated box. Upon reaching the surface, they were immediately transferred to large buckets containing seawater chilled to 7°C and later maintained at atmospheric pressure in a large cooler that contained circulating, aerated seawater chilled to 7°C. Upon return to port, an intact clump of L. cf luymesi was transported in a large cooler containing ice-cold seawater to the University of California at Santa Barbara. The worms were immediately placed in a flow-through aquarium containing seawater at 5°C (pumped from offshore, coarse-filtered and chilled prior to flowing into the aquarium) and a layer of anoxic mud on the bottom of the aquarium. Worms were maintained in this aquarium for several days prior to experimentation. Care was taken to use healthy, active,undamaged worms that had intact `roots' for experimentation(Julian et al., 1999). For experimentation, L. cf luymesi were placed into specially built two-compartment respiration chambers that allowed the posterior and anterior halves of the tubeworms to be isolated into different streams of flowing water (Freytag et al.,2001). All experiments were conducted within 17 days of collection.

Urechis caupo (Fisher and MacGinitie) were collected in November 1996 from the Morro Bay mudflats (35°40′12″N,120°79′93″W) by a suction gun, consisting of a polyvinylchloride (PVC) tube with an [UNK]-ring-sealed plunger designed to extract worms from their burrows. Worms were transported to Santa Barbara in ice-cold seawater and, upon arrival, immediately placed into flowing seawater at 15° (pumped from offshore to our seawater tables). Worms were allowed to acclimate to the water tables for 3 days before experiments began. All experiments on U. caupo were conducted within 8 days of collection.

Measuring metabolite flux by R. pachyptila, L. luymesi and U. caupo

In all experiments, a worm or worms were placed into two respirometry aquaria. A third aquarium always served as a control and was devoid of worms.

In all experiments, seawater was filtered (0.2 μm diameter) and pumped via a metering pump (Cole-Parmer, Inc.) into an acrylic gas equilibration column and bubbled with a combination of CO2, 5%H2S/95% N2, O2 and N2 or He to achieve in situ dissolved gas concentrations (conditions used in experiments are described below). Mass flow controllers (Sierra Instruments,Inc.) regulated the gas flow into the equilibration column. A proportional pH controller and two metering pumps were used to regulate seawater pH (Prominent Industries, Inc.). A sodium nitrate solution (5 mmol l-1) in 0.2μm filter-sterilized seawater was pumped into the equilibration column at a rate that produced in situ seawater nitrate concentrations. The resulting seawater was then pumped from the equilibration column into each aquarium using three high-pressure pumps (for R. pachyptilaexperiments; Lewa America, Inc.) or three metering pumps (for L. cf luymesi and U. caupo experiments; Prominent Industries). Aquarium temperature was maintained by immersion in a circulating waterbath(Fisher Inc.). R. pachyptila aquarium pressure was maintained at 27.5 MPa via pneumatically charged or spring-loaded backpressure valves(Circle Seal, Inc.). Aquarium effluents passed through computer-driven stream selection valves (Valco, Inc.), allowing automated control of effluent analysis.

To determine metabolite flux rates, one seawater stream at a time was directed towards a gas extractor (Fig. 1) that stripped the gases dissolved in the seawater and routed them for analysis by a membrane-inlet mass spectrometer. The extractor is based on the principles of our gas chromatograph seawater inlet(Childress et al., 1984) and allows us to run dissolved gas analyses continuously with higher resolution(3-5 times the sensitivity of analyzing seawater directly; data not shown) and with reduced maintenance of the membrane inlet. To our knowledge, this device is unique in both its design and application in mass spectrometry. In the extractor, seawater was bubbled with helium while being mixed with helium-sparged 20% o-phosphoric acid/80% deionized water. The addition of the degassed phosphoric acid mixture dramatically reduced the pH,converting both inorganic carbon and sulfide species to carbon dioxide and hydrogen sulfide, respectively. A quartz-tipped optical level controller(Levelite Inc.) maintained the fluid level in the extractor. As dissolved gases are extracted from the seawater, they are carried to the membrane-inlet mass spectrometer (Hiden Analytical Inc.) to measure changes in the partial pressures of CO2, H2S, O2 and N2(Kochevar et al., 1992). The mass spectrometer is capable of detecting extremely small changes in partial pressure but, for quantitative determination of metabolite flux, these data were converted into changes in concentration by calibrating the mass spectrometer with a Hewlett-Packard 5890A gas chromatograph(Childress et al., 1984). For the calibrations, 500 μl gas-tight syringes with 30 gauge sideport needles(Hamilton, Inc.) were used to collect seawater samples from water- and gas-tight septa just before the extractor. Regression plots of partial pressure versus total concentration were used to produce standard curves. In all cases, the concentrations used for calibration spanned at least one order of magnitude and encompassed the range of concentrations used in our experiments. In addition, at least 25 samples were collected and used for each chemical parameter; in all cases, r2≥0.90. Calibrated data, as well as the flow rate of the effluent and the total mass of the organisms, were used to determine mass-specific metabolite flux rates.

For all shipboard experiments, R. pachyptila tubeworms were maintained in respirometer aquaria(Kochevar et al., 1992) under in situ conditions (ΣCO2=5-6 mmoll-1,ΣH2S=250-300 μmoll-1, [O2]=100-210μmoll-1, [NO3-]=40-50,μmoll-1, pH 6.5, 12°C, 27.5 MPa) until autotrophy was established. Autotrophy describes a worm that exhibits net inorganic carbon,oxygen and sulfide uptake from the environment, and net elimination of protons into the environment. Autotrophy typically commenced after 12-24 h.

For all experiments with L. cf luymesi, tubeworms were placed in the split-vessel respirometer aquaria(Freytag et al., 2001) and maintained under in situ conditions until autotrophy was established(ΣCO2=2 mmoll-1 in both top and bottom chambers,ΣH2S=500 μmoll-1 in the bottom chamber,[O2] in the top chamber=100-210 μmoll-1,[NO3-]=40-50 μmoll-1 in both top and bottom chambers, pH 6.5 in the bottom chamber, pH 8.0 in the top chamber,12°C, 206 Pa). Autotrophy typically commenced after 48-72 h.

For all experiments with U. caupo, two worms were placed into 38 cm inner diameter Tygon tubing. Both ends of the tubing were fitted with reducing connectors to allow coupling to 0.3 cm diameter polyethylene tubing. Polypropylene plastic mesh was used to prevent the worms from occluding the incurrent and excurrent openings. Metering pumps (Prominent Industries) were used to flush the tubing with chilled, air-saturated seawater from the equilibration column. The entire assembly was placed into a circulating waterbath to maintain the temperature at 15°C. Worms were kept in this tubing (hereafter described as tubing aquaria) with flowing seawater for 12 h prior to experimental manipulations. One tubing aquarium was maintained without worms and served as our control.

To calculate changes in oxygen and sulfide concentration in the seawater caused by U. caupo pre- and post-sulfide exposure, 500 μl gas-tight glass syringes with 30 gauge sideport needles (Hamilton, Inc.) were used to collect seawater samples through gas-tight septa from both experimental and control tubing aquaria. Total dissolved oxygen and sulfide concentrations in each sample were determined by gas chromatography using a Hewlett Packard 5890 gas chromatograph modified for analyzing dissolved gases in seawater (Childress et al.,1984).

After completing in vivo experiments, R. pachyptila, luymesi or U. caupo were removed from the aquaria,weighed on a motion-compensated shipboard balance when at sea(Childress and Mickel, 1980) or an electronic balance (Mettler, Inc.) when on shore, quickly dissected on ice and frozen in liquid nitrogen for further analyses. In most cases, the empty worm tubes were left in the pressure aquaria for several hours and subjected to the same experimental conditions to determine the fraction of the observed flux rates attributable to bacterial growth or to other phenomena associated with the tubes. In this study, as well as previous reports, we have demonstrated no significant contribution of free-living bacteria to our observed metabolite flux rates (Girguis et al., 2000).

Determination of proton elimination rates by R. pachyptila,L. luymesi and U. caupo

To determine the proton elimination rates by R. pachyptila, L. cf luymesi or U. caupo, the seawater pH of the excurrent flows of the experimental and control aquaria was measured by a double-junction pH electrode resistant to interference from sulfide (Broadley-James, Inc.) and an Orion (model 920A) or Radiometer PHM 93 pH meter. In the R. pachyptila and L. cf luymesi experiments, the electrode was housed in an O-ring-sealed acrylic flow-through cell (volume 1.35 ml) with offset inlet and outlet ports to aid in clearing gas bubbles. The effluent stream in the flow-through cell was maintained at 206 kPa to reduce off-gassing, and the assembly was positioned after our automated stream selection valves. pH was measured every 0.25 s, recorded by a computer and averaged over 7.5 min.

In the U. caupo experiments, two worms were placed into the tubing aquaria, one per aquarium, as described above. The entire assembly was placed into a circulating waterbath to maintain the temperature at 15°C. Worms were kept in these tubing aquaria with flowing seawater for 12 h prior to sulfide exposure. One tubing aquarium was maintained without worms and served as our control. For the sulfide exposure experiments, seawater in the equilibration column was bubbled with hydrogen sulfide, to bring the dissolved sulfide concentration up to 100 μmoll-1, and was pumped into the tubing aquaria. Exposure to sulfide continued for 7 h. During this time, the pH of the seawater from the control and experimental aquaria was measured by collecting samples of control and experimental effluent seawater in 60 ml disposable gas-tight syringes every 8-10 min. The effluent was transferred to 125 ml beakers, and the pH was measured with the aforementioned pH electrode and meter.

In seawater, there is considerable buffering by bicarbonate and other inorganic anions. To calculate the organisms' proton elimination rates accurately during the experiments, the buffering of protons by inorganic acid anions had to be considered (because of their relatively low abundance in our seawater, organic acid anions were not considered; Johnson et al., 1988). Equations for the dissociation of carbonic acid, water, boric acid and hydrogen sulfide in seawater as a function of temperature, salinity and pressure were used with our effluent pH measurements, temperature and gas chromatographic measurements of ΣCO2 andΣH2S (as described above) to calculate total alkalinity. The general form of this expression is:
where TA is the total alkalinity. A complete derivation, including the determination of the dissociation constants as a function of pressure, may be found in Millero (1995).

Total alkalinity was then used to calculate the hydrogen ion concentration required to produce the observed differences in the pH between experimental and control aquaria effluents. Total proton elimination rates were then calculated from the hydrogen ion concentrations, the effluent flow rates and the mass of the worms.

Sulfide uptake and proton elimination by R. pachyptila and L. luymesi

During the HOT 96 expedition, three autotrophic R. pachyptilatubeworms, weighing 7-16 g each, were placed into two of the high-pressure respirometry aquaria (one in one aquarium, two in the other aquarium). Dissolved gaseous hydrogen sulfide concentration was changed in the aquarium seawater at specific times over several hours to achieve a series of final seawater sulfide concentrations between 0 and 700 μmol l-1. Worms were kept at each incremental hydrogen sulfide concentration until their sulfide and proton flux rates stabilized (typically 4-7 h). Other than experimental variation in external sulfide concentrations, worms were kept under constant in situ conditions for the duration of the experiment(ΣCO2=5 mmol l-1, [O2]=150 μmol l-1, [NO3-]=40-65 μmol l-1, pH 6.2, 12°C, 27.5 MPa). The above experiment was repeated during the HOT 97 expedition using four freshly collected worms (two in each aquarium).

To determine the effect of sulfide uptake on rates of proton elimination by L. cf luymesi, two tubeworms, weighing 4-6 g each, were placed into one two-chamber respirometry vessel. Although simulating the conditions found in situ utilized the same equipment as in the R. pachyptila experiments, the seawater physico-chemical conditions differed(ΣCO2=1.8 mmol l-1, ΣO2=300μmol l-1, ΣH2S=0 μmol l-1,temperature, 5°C, pressure, 200 Pa). When the oxygen uptake of luymesi stabilized, dissolved gaseous hydrogen sulfide was added to the posterior chamber fluid of the respirometer vessels in two increments over several hours to achieve final seawater sulfide concentrations of first 238 μmol l-1 and then 515 μmol l-1. Aquaria conditions were kept at each incremental sulfide concentration until the worms' sulfide and proton flux rates stabilized, typically 2-5 h. Other than experimental variation in external sulfide concentrations, worms were kept under constant in situ conditions for the duration of the experiment.

Inorganic carbon uptake and proton elimination by R. pachyptila

During the HOT 97 expedition, four autotrophic R. pachyptilatubeworms, weighing 4-15 g each, were placed into two high-pressure aquaria(two per aquarium). Inorganic carbon concentration in the seawater was increased from 3 to 10 mmol l-1 over 6 h. The inorganic carbon concentration was then reduced to 4.4±0.12 mmol l-1 and maintained for 11 h to accustom the tubeworms to the lower environmental inorganic carbon concentration. Finally, the inorganic carbon concentration in the seawater was increased to 8.7 mmol l-1, kept at this concentration for 10 h and then decreased to 2.1 mmol l-1 over 4 h.

Oxygen uptake and proton elimination by R. pachyptila

During the HOT 96 expedition, four autotrophic R. pachyptilatubeworms, weighing 6-8 g each, were placed into two high-pressure aquaria(two per aquarium). Sulfide concentration in the aquaria was maintained at 210-250 μmol l-1, while dissolved seawater oxygen concentration was decreased from 350 to 78 μmol l-1 over 8 h. During the HOT 97 and LARVE 98 expeditions, three autotrophic R. pachyptilatubeworms were placed into two aquaria (one in one aquarium, two in the other)and the dissolved oxygen concentration was then decreased from 394 and 314μmol l-1, respectively, to <3 μmol l-1(Childress et al., 1984) over 11 and 13 h, respectively.

The effects of transport inhibitors on proton elimination byR. pachyptila

During the HOT 96, HOT 97 and LARVE 98 expeditions, four inhibitors of ATPases, N-ethylmaleimide (NEM), amiloride, lansoprazole and vanadate, were used in separate treatments on R. pachyptila to assess the mechanism by which protons are being eliminated into the environment. NEM non-selectively inhibits ATPases and is a general metabolic poison(Hilden and Madias, 1991). amiloride inhibits Na+ exchange(Kleyman and Cragoe, 1988). Vanadate inhibits P-type ATPases(Chatterjee et al., 1992). Lansoprazole is highly specific to and inhibits P-type K+/H+-ATPases(Tomiyama et al., 1994). In each treatment, 2-5 autotrophic R. pachyptila worms, weighing 4-14 g each, were placed into two of the three high-pressure aquaria (one, two or three worms per aquarium). In all treatments, inhibitors were introduced into the system before the high-pressure pumps to prevent depressurization of the respirometer chambers. Inhibitors were dissolved in 10 ml of deionized water,except for amiloride, which was dissolved in 10% dimethylsulfoxide (DMSO) in deionized water. The seawater concentrations of NEM, amiloride, lansoprazole and vanadate in these respirometer chambers were 2 mmol l-1, 1 mmol l-1, 2 mmol l-1 and 750 μmol l-1,respectively (Goffredi, 1998). After addition of the inhibitor, the worms were left uninterrupted for at least 6 h while seawater dissolved gas concentrations and pH were being measured. The aquaria were continuously flushed by seawater under in situ conditions.

The effects of sulfide exposure on proton elimination by R. pachyptila, L. cf luymesi and U. caupo

Data from the HOT 96 and HOT 97 expeditions demonstrated that an increased seawater sulfide concentration resulted in increased sulfide uptake by R. pachyptila and established the relationship between environmental sulfide concentrations and sulfide uptake rate (y=1.04+0.0148x, r2=0.91, P=0.825, where y is sulfide uptake rate in μmol g-1 h-1 and x is environmental total sulfide concentration in μmol l-1; data from Fig. 2). In addition, increased proton elimination rate by R. pachyptila correlated strongly with increased sulfide uptake (r2=0.87; Fig. 2A) for seawater sulfide concentrations of 0-700 μmol l-1(Fig. 2B). The correlation between sulfide uptake and proton elimination rates appeared to remain linear between 0 and 700 μmol l-1. Proton elimination rate changed rapidly in response to changes in environmental sulfide concentration, with proton elimination increasing within 20 min of an increase in seawater sulfide concentration (Table 1). Oxygen uptake rate prior to exposure to sulfide was approximately 4.81 μmol g-1 h-1, and increased by 280% after sulfide had been introduced to the aquaria (Table 1). Removing sulfide from the aquarium seawater stopped inorganic carbon uptake and reduced oxygen uptake and proton elimination by approximately 50% and 90%, respectively(Fig. 3).

In our experiments on L. cf luymesi, exposure to hydrogen sulfide induced proton elimination from nearly undetectable rates to 11.90±0.94 μequiv g-1 h-1 (mean ± S.D., N=14) (Table 1). Increasing the total sulfide concentration in the bottom chamber water from 238±44 μmol l-1 to 515±71 μmol l-1total H2S resulted in a concomitant increase in proton elimination in the top chamber from 5.31±1.39 μmol g-1 h-1to 11.90±0.94 μmol g-1 h-1, respectively. In addition, the increased total sulfide in the bottom chamber led to a corresponding increase in sulfide uptake by L. cf luymesi in the bottom chamber (from 0.91±1.2 μmol g-1 h-1to 2.6±0.2 μmol g-1 h-1, respectively). Due to technical difficulties, proton elimination by L. cf luymesiinto the bottom chamber of the aquaria was not measured.

Prior to sulfide exposure, U. caupo exhibited no proton elimination (Table 1). Upon exposure to 100 μmol l-1 sulfide, U. caupo exhibited a modest rate of proton elimination into the environment for approximately 45 min (2.17±1.06 μmol g-1 h-1; mean ±S.D., N=5) (Table 1). The rate of proton elimination was five- and 20-fold lower than that of L. cf luymesi and R. pachyptila respectively. The sulfide oxidation rate by the worm, more specifically the quantity of sulfide detoxified by the worm, was not determined. A comparison of oxygen uptake rates for the two U. caupo groups, one group maintained in seawater and the other exposed to 100 μmol l-1 sulfide in seawater,showed a significant difference: 3.12±0.19 and 4.55±0.61 μmol g-1 h-1, respectively (means ± S.D., P<0.0001; N=4 worms per group; Mann—Whitney U-test).

The effects of inorganic carbon uptake on proton elimination by R. pachyptila

In our experiments, proton elimination rates correlated with changes in environmental inorganic carbon concentrations only while environmental inorganic carbon concentrations were in flux(Fig. 4). Continuously increasing the inorganic carbon concentration in the respirometer aquaria resulted in short-lived, but continuously increasing, proton elimination rates(Fig. 4A), while continuously decreasing the environmental inorganic carbon concentration decreased proton elimination rates by R. pachyptila(Fig. 4C). When environmental inorganic carbon concentrations were held constant, there was no correlation between inorganic carbon uptake and proton elimination rates(Fig. 4B). During all three inorganic carbon regimes, seawater sulfide concentrations and R. pachyptila sulfide uptake rates remained constant.

The effects of oxygen uptake on proton elimination by R. pachyptila

One of three experiments demonstrated a near-cessation of proton elimination when environmental oxygen tensions decreased from 350 to 78μmol l-1 (Table 2). In this experiment, oxygen uptake was diminished from 12.4±3.6 μmol g-1 h-1 to 1.9±4.3μmol g-1 h-1 (means ± S.D., N=17-19). In two later experiments, proton elimination rates nearly ceased when oxygen tension was reduced to 5 μmol l-1 or below(Childress et al., 1984). In these two experiments, the reduced oxygen tensions eliminated sulfide uptake by R. pachyptila (Table 2).

The effects of transport inhibitors on proton elimination byR. pachyptila

With the exception of lansoprazole, exposure to all ATPase inhibitors reduced proton elimination rates by at least 96%(Table 3). Amiloride, vanadate and NEM all led to the cessation of proton elimination by R. pachyptila, as well as eliminating inorganic carbon uptake and drastically reducing sulfide and oxygen uptake rates (for example, see Fig. 5). Exposure of R. pachyptila to lansoprazole reduced proton elimination rate by 17.8%(Table 3), and reduced sulfide uptake rates by 26%. Lansoprazole did not, however, affect the inorganic carbon or oxygen uptake rates of R. pachyptila.

The necessity to maintain an intracellular pH appropriate for enzyme function mandates that organisms eliminate excess protons. The buffering of protons is an effective mechanism for preventing the onset of acidosis during transient proton production, e.g. during anaerobic metabolism(Heisler, 1993). However, the symbionts of R. pachyptila and L. cf luymesicontinuously oxidize sulfide when the organisms are maintained in typical vent conditions (approximately 5 mmol l-1 ΣCO2, 110μmol l-1 ΣH2S, 100 μmol l-1O2, [NO3-]=40 μmol l-1, pH 5.9,12.5°C), and this leads to sustained proton production(Fig. 2) and elimination. Because proton elimination rates correlate strongly with sulfide uptake rates and are drastically reduced when oxygen or sulfide levels are experimentally depleted in the aquarium seawater, we suggest that proton elimination is driven by the oxygen-dependent sulfide oxidation of the symbiont. Our in vivo experiments, conducted under environmentally relevant conditions,support the results of previous in vitro studies(Childress et al., 1991; Scott et al., 1998). Proton elimination by R. pachyptila constitutes the largest mass-specific metabolite flux measured for this species. Proton elimination by L. cf luymesi also appears to be the highest metabolite flux for the species, although a full respirometric study of L. cf luymesi remains to be completed.

R. pachyptila, however, does experience anoxia in situ,and we chose to examine the contribution of anaerobic metabolism to proton elimination. In two of our hypoxia experiments, R. pachyptilamaintained at an oxygen concentration below 5 μmol l-1demonstrated drastically reduced proton elimination rates and undetectable inorganic carbon and sulfide uptake rates(Table 2). Protons eliminated by R. pachyptila during these experiments are probably the result of anaerobic metabolism, i.e. glycolysis, and are 1-14% of proton elimination rates observed during aerobic sulfide oxidation. Although previous studies have shown that R. pachyptila can survive anoxic conditions for 60 h(Arndt et al., 1998; Goffredi, 1998), our experiments demonstrate that symbiont function ceases very quickly and that symbiont metabolism cannot be sustained in the absence of oxygen(Fig. 3).

In one of our oxygen experiments, lowering environmental oxygen concentrations to 78 μmol l-1 resulted in the cessation of proton elimination, inorganic carbon uptake and sulfide uptake. It is possible that net proton elimination is an oxygen-dependent process (such as a redox proton pump; Steinmetz and Andersen,1982). However, we suggest that it is more likely that proton elimination is indirectly dependent on energy from aerobic metabolism. Proton elimination by the intertidal worm Sipunculus nudus was induced by anaerobic conditions, but the subsequent proton elimination correlated with overall metabolic rate, in particular with aerobic respiration rate(Portner et al., 1991). We agree with these authors that there is a correlation between energy-consuming ion translocation and energy availability, which is primarily derived from aerobic metabolism.

In situ, R. pachyptila will also encounter large fluctuations in environmental CO2 concentrations (from 2.1 to 7.1 mmol l-1; Childress et al.,1993; Goffredi et al.,1997; Johnson et al.,1988). Inorganic carbon acquisition by R. pachyptilaoccurs by diffusion of CO2 from the environment into the vascular blood, so maintaining the internal pH alkaline relative to the environment is paramount to sustaining inorganic carbon acquisition(Goffredi et al., 1997). Our experiments showed that proton elimination by R. pachyptila was not correlated with inorganic carbon concentrations when all environmental metabolite concentrations were maintained at constant levels(Fig. 4). Under these conditions, there is no net production of protons from the acquisition and transport of inorganic carbon by R. pachyptila because CO2is converted to bicarbonate in the vascular blood and is reconverted to CO2 for use by the symbionts(Scott et al., 1999). However,transient increases in CO2 concentration induced proton elimination(Fig. 4), andR. pachyptila appeared to eliminate protons while the internal and environmental pools of inorganic carbon pools were equilibrating. We suggest that protons are eliminated to control pH as the bicarbonate concentration increases in the vascular blood. This response is similar to that of organisms experiencing hypercapnia (Arndt et al.,1998; Goffredi et al.,1999; Kochevar et al.,1991).

Interestingly, proton elimination by R. pachyptila will also effectively reduce the pH of the seawater in contact with the gill, further favoring the passive influx of carbon dioxide. In addition to the large surface area and the presence of abundant carbonic anhydrase(Goffredi et al., 1999; Kochevar et al., 1991), this mechanism may further enhance the ability of R. pachyptila to acquire inorganic carbon for its symbionts.

In the low-pH vent environment (Johnson et al., 1988), proton elimination by R. pachyptila occurs against a concentration gradient, so the process must be coupled to ATP hydrolysis. The transport of protons may occur via a cation exchanger, e.g. a Na+/H+ exchanger, or via a proton-translocating ATPase (Tomiyama et al., 1994). During our inhibitor experiments, both vanadate and amiloride (Fig. 5) were very effective at inhibiting proton elimination, suggesting that P-type ATPases and possibly Na+/H+-ATPases are involved in net proton elimination. The rates of inorganic carbon and sulfide uptake were also reduced, suggesting that symbiont metabolic processes are disrupted by the cessation of proton elimination (Fig. 5). However, the potential of these inhibitors to inhibit Na+/K+-ATPases and disrupt cellular Na+concentrations is a confounding factor. Lansoprazole, a highly specific K+/H+-ATPase inhibitor(Sachs et al., 1995),inhibited 17.8% of proton elimination, demonstrating the role of K+/H+-ATPases in proton elimination(Table 3). These are the first live-animal experiments detailing the rates and mechanisms of proton exchange by any deep-sea organism, and they suggest that R. pachyptilapossesses both K+/H+-ATPases and Na+/H+-ATPases that are involved in sulfide-driven proton elimination. A recent in vitro study of frozen R. pachyptila tissues has shown high activities of ATPases in the plume, as well as activities of both K+/H+-ATPases and Na+/H+-ATPases(Goffredi and Childress,2001). Although that study estimated that 2-6% of the total ATPases are K+/H+-ATPases, the present results suggest that a larger percentage of the ATPases of R. pachyptila are K+/H+-ATPases. It is difficult to determine whether the discrepancy is due to incomplete efficacy of inhibitors in the whole animal or in vitro experiments. In addition, proton elimination by H+-ATPases, e.g. electrogenic proton pumps, cannot be ruled out because they may also account for a large fraction of proton elimination.

The protons generated by symbiont sulfide oxidation are not coupled to oxidative phosphorylation (as are the proton by-products of anaerobic metabolism; Hochachka and Somero,1984) and are primarily a waste product of symbiont metabolism. Disposing of these protons may represent a large fraction of the energetic costs of R. pachyptila and L. cf luymesi. H+-translocating ATPases usually translocate 1-3 protons per ATP hydrolyzed (Steinmetz and Andersen,1982) and at typical R. pachyptila proton elimination rates (Table 3), between 12 and 15 μmol g-1 h-1 ATP should be utilized in proton exchange. Using a ratio of 6.2 ATP per mole of O2 for aerobic metabolism (Hochachka and Somero,1984), 2.4μmol O2 g-1 h-1 is involved in ATP synthesis for proton elimination. This accounts for approximately 25% of the oxygen taken up by R. pachyptila and 60% of the host's oxygen consumption (determined by eliminating sulfide from the environment and stopping symbiont autotrophic metabolism).

Proton elimination and sulfide uptake rates by R. pachyptila are 4-7 times higher than the corresponding rates by L. cf luymesi when both are exposed to comparable environmental levels of sulfide (approximately 500 μmol l-1; Figs 3, 4). The conservation in the stoichiometry of protons produced per sulfide oxidized by R. pachyptila and L. cf luymesi (5.82±0.17 and 3.84±0.59 protons per sulfide, respectively) suggests that the symbiont pathways of sulfide oxidation may be similar. Our experimental results fall within the range of theoretical and experimental models of the number of protons generated per sulfur oxidized (see fig. 3 in Nelson and Hagen, 1995). Individual variation in these values may result from differences in the rates of reduction of inorganic carbon and nitrogen(Girguis et al., 2000).

Our experiments with U. caupo illustrate the pronounced difference in proton elimination rates between chemoautotrophic symbioses and non-symbiotic metazoans. Although we did not quantify the rate of sulfide oxidation of U. caupo, we have demonstrated that exposure to sulfide induced proton elimination as well as a significant (P<0.05)change in oxygen consumption rates (Table 1). However, proton elimination by U. caupo was short-lived, lasting less than 45 min, and was typically 5-20 times less rapid than in L. cf luymesi and R. pachyptila(Table 1). Non-symbiotic organisms that reside in chemically reduced habitats may exhibit ephemeral proton elimination resulting from the oxidative detoxification of reduced substrates and environmentally induced hypercapnia (e.g. sipunculid worms; Pörtner et al., 1991) but do not require high sustained rates of proton elimination. A previous study found that proton elimination by Sipunculus audus averaged 0.08-0.32μmol g-1 h-1 and lasted for nearly 3 days(Portner et al., 1991). U. caupo, however, exhibited much higher, albeit shorter-lived, proton elimination rates. Neither S. audus nor U. caupo exhibited rates comparable with those of R. pachyptila or L. cf luymesi.

Although proton concentrations in organisms are typically 3-5 orders of magnitude lower than those of the most prevalent cytoplasmic ions(Hochachka and Somero, 1984),the ability to regulate intracellular and extracellular pH is ubiquitous amongst organisms. Chemoautotrophic symbioses, in particular those of R. pachyptila and others with a relatively high metabolite flux, must contend with the continuous net production of protons by the symbionts and of protons produced by their own metabolism. As the principal waste product of sulfide oxidation, the ability of the host rapidly and efficiently to eliminate protons produced by sulfur metabolism is a necessary adaptation to this mode of symbiosis. While the incurred metabolic costs of proton elimination by R. pachyptila and L. cf luymesiappear to be tremendous, protons are the primary end-product of sulfur oxidation. The rapid and copious elimination of protons by vestimentiferans is an essential adaptation to symbiosis with sulfide-oxidizing bacteria, the absence of which would result in the rapid acidification and eventual metabolic dysfunction of both host and symbiont.

The authors would like to thank Dr Charles Fisher for generously providing the Lamellibrachia used in this research, as well as the captains and crew of the RV New Horizon, RV Atlantis II and RV Atlantis, DSRV Alvin, RV Wecoma, RV Nadir and DSRV Nautile. We also thank Dr F. Zal and S. Goffredi for their tireless efforts, as well as Dr R. Trench and Dr R. Suarez for their reviews and revisions of this manuscript. We are indebted to Dr Qais Al-Awqati for his comments and insight. Special thanks go to Dr F. Gaill, Dr A. Chave and Dr D. Manahan, chief scientists of the 1996, 1997 and 1998 expeditions. Funding for this project was provided by NSF grants OCE-9301374 (J.J.C.), OCE-9632861 (J.J.C.) and OCE-002464 (J.J.C.).

Arndt, C., Schiedek, D. and Felbeck, H. (
). Anaerobiosis in the hydrothermal vent tubeworm Riftia pachyptila.
Cah. Biol. Mar.
Cavanaugh, C. M., Gardiner, S. L., Jones, M. L., Jannasch, H. W. and Waterbury, J. B. (
). Prokaryotic cells in the hydrothermal vent tube worm Riftia pachyptila: Possible chemoautotrophic symbionts.
Chatterjee, D., Chakraborty, M., Leit, M., Neff, L.,Jamsa-Kellokumpu, S., Fuchs, R. and Baron, R. (
). Sensitivity to vanadate and isoforms of subunits A and B distinguish the osteoclast proton pump from other vacuolar proton ATPases.
Proc. Natl. Acad. Sci. USA
Childress, J. J., Arp, A. J. and Fisher, C. R., Jr(
). Metabolic and blood characteristics of the hydrothermal vent tubeworm Riftia pachyptila.
Mar. Biol. (Berlin)
Childress, J. J. and Fisher, C. R. (
). The biology of hydrothermal vent animals: physiology, biochemistry, and autotrophic symbioses.
Oceanogr. Mar. Biol. Annu. Rev.
Childress, J. J., Fisher, C. R., Favuzzi, J. A., Kochevar, R. E., Sanders, N. K. and Alayse, A. M. (
). Sulfide-driven autotrophic balance in the bacterial symbiont-containing hydrothermal vent tubeworm Riftia pachyptila.
Biol. Bull.
Childress, J. J., Lee, R. W., Sanders, N. K., Felbeck, H., Oros,D. R., Toulmond, A., Desbruyeres, D., Kennicutt, M. C. and Brooks, J.(
). Inorganic carbon uptake in hydrothermal vent tubeworms facilitated by high environmental partial pressure of carbon dioxide.
Childress, J. J. and Mickel, T. J. (
). A motion compensated shipboard precision balance system.
Deep-Sea Res.
Felbeck, H. (
). Chemoautotrophic potential of the hydrothermal vent tube worm, Riftia pachyptila Jones(Vestimentifera).
Freytag, J. K., Girguis, P. R., Bergquist, D. C., Andras, J. P.,Childress, J. J. and Fisher, C. R. (
). A paradox resolved: Sulfide acquisition by roots of seep tubeworms sustains net chemoautotrophy.
Proc. Natl. Acad. Sci. USA
Girguis, P. P. and Childress, J. J. (
). H+ equivalent elimination by the tubeworm Riftia pachyptila: Ramifications for inorganic metabolite flux.
Cah. Biol. Mar.
Girguis, P. R., Lee, R. W., Desaulniers, N., Childress, J. J.,Pospesel, M., Felbeck, H. and Zal, F. (
). Fate of nitrate acquired by the tubeworm Riftia pachyptila.
Appl. Environ. Microbiol.
Goffredi, S. K. (
Physiological adaptations of the hydrothermal vent tubeworm Riftia pachyptila:including the uptake of carbon dioxide and hydrogen sulfide, unprecedented pH regulation, and a reverse chloride shift
. Dissertation: University of California Santa Barbara.
Goffredi, S. K. and Childress, J. J. (
). Activity and inhibitor sensitivity of ATPases in the hydrothermal vent tubeworm Riftia pachyptila: a comparative approach.
Mar. Biol.
Goffredi, S. K., Childress, J. J., Desaulniers, N. T., Lee, R. W., Lallier, F. H. and Hammond, D. (
). Inorganic carbon acquisition by the hydrothermal vent tubeworm Riftia pachyptiladepends upon high external PCO2 and upon proton-equivalent ion transport by the worm.
J. Exp. Biol.
Goffredi, S. K., Girguis, P. R., Childress, J. J. and Desaulniers, N. T. (
). Physiological functioning of carbonic anhydrase in the hydrothermal vent tubeworm Riftia pachyptila.
Biol. Bull.
Heisler, N. (
). Acid-base regulation. In
The Physiology of Fishes
, vol.
(ed. D. H. Evans), pp.
-378. Salem, MA: CRC Marine Science Series.
Hilden, S. A. and Madias, N. E. (
). Effect of sulfhydryl compounds on ATP-stimulated proton transport and chlorine uptake in rabbit renal cortical endosomes.
J. Membr. Biol.
Hochachka, P. W. and Somero, G. N. (
Biochemical Adaptation
. Princeton, NJ: Princeton University Press.
Johnson, K. S., Childress, J. J., Hessler, R. R.,Sakamoto-Arnold, C. M. and Beehler, C. L. (
). Chemical and biological interactions in the Rose Garden [eastern Pacific Ocean]hydrothermal vent field, Galapagos spreading center.
Deep-Sea Res. Part A Oceanogr. Res. Papers
Jones, M. L. (
). Riftia pachyptila, new genus, new species, the vestimentiferan tubeworm from the Galapagos Rift geothermal vents.
Proc. Biol. Soc. Wash
Julian D., Gaillo, F., Wood, E., Arp, A. J. and Fisher, C. R. (
). Roots as a site of hydrogen sulfide uptake in the hydrocarbon seep vestimentiferan Lamellibrachia sp.
J. Exp. Biol.
Kennicutt II, M. C., Brooks, J. M., Bidigare, R. R., Fay, R. R.,Wade, T. L. and McDonald, T. J. (
). Vent-type taxa in a hydrocarbon seep region on the Louisiana slope.
Kleyman, T. R. and Cragoe, E. J., Jr (
). Amiloride and its analogs as tools in the study of ion transport.
J. Membr. Biol.
Kochevar, R. E., Childress, J. J., Fisher, C. R. and Minnich,E. (
). The methane mussel: Roles of symbiont and host in the metabolic utilization of methane.
Mar. Biol.
Kochevar, R. E., Childress, J. J. and Govind, N. S.(
). Carbonic anhydrase in the hydrothermal vent tubeworm Riftia pachyptila Jones.
Am. Zool.
Macdonald, I. R., Boland, G. S., Baker, J. S., Brooks, J. M.,Kennicutt II, M. C. and Bidigare, R. R. (
). Gulf of Mexico hydrocarbon seep communities. II. Spatial distribution of seep organisms and hydrocarbons at Bush Hill [Louisiana, USA].
Mar. Biol.
Menon, J. G. and Arp, A. J. (
). Ultrastructural evidence of detoxification in the alimentary canal of Urechis caupo.
Invert. Biol.
Mickel, T. J. and Childress, J. J. (
). Effects of pressure and temperature on the EKG and heart rate of the hydrothermal vent crab Bythograea thermydron (Brachyura).
Biol. Bull.
Millero, F. J. (
). Thermodynamics of the carbonate system in the oceans.
Geochim. Cosmochim. Acta
Nelson, D. C. and Hagen, K. D. (
). Physiology and biochemistry of symbiotic and free-living chemoautotrophic sulfur bacteria.
Am. Zool.
Pörtner, H. O., Andersen, N. A. and Heisler, N.(
). Proton-equivalent ion transfer in Sipunculus nudus as a function of ambient oxygen tension: relationships with energy metabolism.
J. Exp. Biol.
Sachs, G., Shin, J. M., Briving, C., Wallmark, B. and Hersey,S. (
). The pharmacology of the gastric acid pump: The H+K+ ATPase-1,2. In
Annual Review of Pharmacology and Toxicology
(ed. A. K. E. Cho), pp.
-305. Palo Alto, CA: Annual Reviews Inc.
Scott, K. M., Bright, M. and Fisher, C. R.(
). The burden of independence: Inorganic carbon utilization strategies of the sulphur chemoautotrophic hydrothermal vent isolate Thiomicrospira crunogena and the symbionts of hydrothermal vent and cold seep vestimentiferans.
Cah. Bio. Mar.
Scott, K. M., Bright, M., Macko, S. A. and Fisher, C. R.(
). Carbon dioxide use by chemoautotrophic endosymbionts of hydrothermal vent vestimentiferans: Affinities for carbon dioxide, absence of carboxysomes, and delta 13C values.
Mar. Biol.
Steinmetz, P. R. and Andersen, O. S. (
). Electrogenic proton transport in epithelial membranes.
J. Membr. Biol.
Tomiyama, Y., Morii, M. and Takeguchi, N.(
). Specific proton pump inhibitors E3810 and lansoprazole affect the recovery process of gastric secretion in rats differently.
Biochem. Pharmacol.
Wilmot, D. B., Jr and Vetter, R. D. (
). The bacterial symbiont from the hydrothermal vent tubeworm Riftia pachyptila is a sulfide specialist.
Mar. Biol.