The study of regeneration in armed echinoderm species, including crinoids, ophiuroids and asteroids, is attracting increasing attention. Recent interest has focused on the presence and potential role of growth factors, including members of the nerve growth factor (NGF) and transforming growth factor-beta (TGF-beta) families, in the regenerative process and their possible relationship to the normal developmental (ontogenetic) regulatory cascade. In addition, the expression patterns of the heat-shock family of stress proteins (Hsps) during regeneration are also important. Their role forms part of a normal stress response to the trauma of autotomy in combination with a putative function in tissue remodelling and associated protein turnover during regeneration. The temporal dynamics of the stress response may also be strongly indicative of environmentally adaptive pressures operating on these systems.
REFERENCES
Angerer
L. M.
, Angerer
R. C.
(2000
). Animal—vegetal axis patterning mechanisms in the early sea urchin embryo.
Dev. Biol
218
, 1
–.Angerer
L. M.
, Oleksyn
D. W.
, Logan
C. Y.
, McClay
D. R.
, Dale
L.
, Angerer
R. C.
(2000
). A BMP pathway regulates cell fate allocation along the sea urchin animal—vegetal embryonic axis.
Development
127
, 1105
–.Becker
J.
, Craig
E. A.
(1994
). Heat-shock proteins as molecular chaperones.
Eur. J. Biochem
219
, 11
–.Cross
M.
, Dexter
T. M.
(1991
). Growth factors in developmental transformation and tumorigenesis.
Cell
64
, 271
–.Dale
K.
, Sattar
N.
, Heemskerk
J.
, Clarke
J. D. W.
, Placzec
M.
, Dodd
J.
(1999
). Differential patterning of ventral midlineFig.2. Immunocytochemical localization of TGF- 1 using the (MAS729) antibody in regenerating arms of Antedon mediterranea. Strong immunoreactivity is observed in the regenerating blastema (arrowhead) and weaker immunoreactivity in the brachial nerve (long arrow) and coelomic epithelium (short arrow) at 1 week post-amputation. Scale bar, 100m. 848cells by axial mesoderm is regulated by BMP7 and chordin.
Development
126
, 397
–.Feder
M. E.
, Hofmann
G. E.
(1999
). Heat-shock proteins, molecular chaperons, and the stress response: evolutionary and ecological physiology.
Annu. Rev. Physiol
61
, 243
–.Feder
M. E.
(1999
). Organismal, ecological, and evolutionary aspects of heat-shock proteins and the stress response: established conclusions and unresolved issues.
Am. Zool
39
, 857
–.Feder
M. E.
(1999
). Engineering candidate genes in studies of adaptation: the heat-shock protein Hsp70 in Drosophila melanogaster.
Am. Nat
154
, 55
–.Flann
S.
, Hawkes
R. B.
, Riederer
B. M.
, Rider
C. C.
, Beesley
P. W.
(1997
). Changes in ubiquitin immunoreactivity in developing rat brain: a putative role for ubiquitin and ubiquitin conjugates in dendrite outgrowth and differentiation.
Neuroscience
81
, 173
–.Garcia-Arraras
J. E.
, Estrada-Rodgers
L.
, Santiago
R.
, Torres
I.
, Diaz-Miranda
L.
, Torres-Avillan
I.
(1998
). Cellular mechanisms of intestine regeneration in the sea-cucumber Holothuria glaberrina Selenka (Holothuroidea: Echinodermata).
J. Exp. Zool
281
, 288
–.Garcia-Arraras
J. E.
, Diaz-Miranda
L.
, Torres
I. I.
, File
S.
, Jimenez
L. B.
, Rivera-Bermudez
K.
, Arroyo
E. J.
, Cruz
W.
(1999
). Regeneration of the enteric nervous system in the sea cucumber Holothuria glaberrina.
J. Comp. Neurol
406
, 461
–.Hofmann
G. E.
, Somero
G. N.
(1995
). Evidence for protein damage at environmental temperatures: seasonal changes in levels of ubiquitin conjugates and hsp70 in the intertidal mussel Mytilus trossulus.
J. Exp. Biol
198
, 1509
–.Hofmann
G. E.
(1999
). Ecologically relevant variation in induction and function of heat shock proteins in marine organisms.
Am. Zool
39
, 889
–.Hogan
B. L. M.
(1996
). Bone morphogenetic proteins: multifunctional regulators of vertebrate development.
Genes Dev
10
, 1580
–.Hwang
S. P. L.
, Chen
C. A.
, Chen
C. P.
(1999
). Sea urchin TgBMP2/4 gene encoding a bone morphogenetic protein closely related to vertebrate BMP2 and BMP4 with maximal expression at the later stages of embryonic development.
Biochem. Biophys. Res. Comm
258
, 457
–.Jasinskiene
N.
, Jasinskas
A.
, Langmore
J. P.
(1995
). Embryonic regulation of histone ubiquitination in the sea urchin.
Dev. Genet
16
, 278
–.Kingsley
D. M.
(1994
). The TGF-superfamily: new members, new receptors, and new genetic tests of function in different organisms.
Genes Dev
8
, 133
–.Kovarik
A.
, Hlubinova
K.
, Vrbenska
A.
, Prachar
J.
(1987
). An improved colloidal silver staining method of protein blots on nitrocellulose membranes.
Folia Biol
33
, 254
–.Laemmli
U. K.
(1970
). Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature
227
, 680
–.Massague
J.
, Wotton
D.
(2000
). Transcriptional control by the TGF-/Smad signalling system.
EMBO J
19
, 1745
–.Mimnaugh
E. G.
, Bonvini
P.
, Neckers
L.
(1999
). The measurement of ubiquitin and ubiquitinated proteins.
Electrophoresis
20
, 418
–.Moss
C.
, Hunter
A. J.
, Thorndyke
M. C.
(1998
). Patterns of bromodeoxyuridine incorporation and neuropeptide immunoreactivity in the regenerating arm of the starfish Asterias rubens.
Phil. Trans. R. Soc. Lond. B
353
, 421
–.Nguyen
Vu. H.
, Trout
J.
, Connors
S. A.
, Andermann
P.
, Weinberg
E.
, Mullins
M. C.
(2000
). Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signalling pathway.
Development
127
, 1209
–.Nodder
S.
, Martin
P.
(1997
). Wound healing in embryos: a review.
Anat. Embryol
195
, 215
–.O'Kane
S.
, Ferguson
M. W. J.
(1997
). Transforming growth factors and wound healing.
Int. J. Biochem. Cell. Biol
29
, 63
–.Pickart
C. M.
, Summers
R. G.
, Shim
H.
, Kasperek
M.
(1991
). Dynamics of ubiquitin pools in developing sea urchin embryos.
Dev. Growth Diff
33
, 587
–.Ponce
M. R.
, Micol
J. L.
, Peterson
K. J.
, Davidson
E. H.
(1999
). Molecular characterization and phylogenetic analysis of SPBMP5-7, a new member of the TGF-superfamily expressed in sea urchin embryos.
Mol. Biol. Evol
16
, 634
–.Sköld
M.
, Rosenberg
R.
(1996
). Arm regeneration frequency in eight species of Ophiuroidea (Echinodermata) from European sea areas.
J. Sea Res
35
, 353
–.Stenzel
P.
, Angerer
L. M.
, Smith
B. J.
, Angerer
R. C.
, Vale
W. W.
(1994
). The univin gene encodes a member of the transforming growth factor-superfamily with restricted expression in the sea urchin embryo.
Dev. Biol
166
, 149
–.Towbin
H.
, Staehelin
T.
, Goedon
J.
(1979
). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc. Natl. Acad. Sci. USA
76
, 4350
–.Vassilev
A. P.
, Rasmussen
H. H.
, Christensen
E. I.
, Nielsen
S.
, Celis
J. E.
(1995
). The levels of ubiquitinated histone H2A are highly upregulated in transformed human cells: partial colocalization of uH2A clusters and PCNA/cyclin foci in a fraction of cells in S-phase.
J. Cell Sci
108
, 1205
–.
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