In vitro tissue culture is shown to be a possible mode of experimentation with the tissues of the Blow Fly larva. Methods are described-whereby the tissues, and the body fluids requisite as culture media may be obtained free from bacteria. The imperfections of the technique are noted and the conclusion reached that a successful technique must depend on the rearing of bacteria-free larvae, for which a method is briefly outlined. It is shown that progress in this part of the work must await further physiological knowledge, particularly in respect to the nature of the body fluids.

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Manufactured by Necol Industrial Collodions Ltd., Stowmarket, Suffolk. The writer is indebted to the local manager of the firm for advice concerning a collodion suitable for the production of membranes of high grade permeability.

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