ABSTRACT
Various flagellates and small ciliates stick to the axopods of Actinophrys. Contact with the base of an axopod or with the body surface leads to the outgrowth of a food funnel, by which the prey is ultimately surrounded.
If a fine probe or micropipette touches the body surface or the base of an axopod, a small cup may grow out over it, investing it closely, but the instrument is soon released. A squirt of tap water from a micropipette may also provoke the outgrowth of a small lobe or cup, or local pinocytosis.
Contact with, or a squirt from, a micropipette containing a solution of egg albumin provokes a more extensive reaction. The micropipette usually becomes invested extensively. The micropipette is drawn into the body and held there for up to an hour.
Immersion in egg albumin solution leads to a temporary spreading and lobulation of the axopod bases, and later a ‘skin’ may separate from the body surface. Skin formation is more pronounced in serum albumin solution, and may also be induced by y-globulins and gelatin.
On treatment in vivo with toluidine blue or thionine a violet layer in the body surface separates as a pinkish violet or violet skin, leaving the body surface unstained.
There is evidence that the skin-forming substance is associated directly or indirectly with the maintenance of cell shape.
