The study of the nutritional requirements of mosquito larvae has made comparatively little progress since Trager (1935) reported that they could be brought to maturity in a sterile medium consisting only of autoclaved yeast and liver extract. Subsequently a number of random observations were reported, dealing with the factors necessary for growth and metamorphosis of mosquito larvae. No concerted effort, however, has been made to replace the yeast-liver extract medium of Trager by a mixture of known components. This is the immediate object of our investigations, and the results presented below serve to bring it yet another stage nearer realization.

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