Using an antiserum directed against γ-aminobutyric acid (GABA) to label neurones with GABA-like immunoreactivity, approximately 70 central neurones (68±9; mean ± S.E.M., N=9) were labelled in the terminal abdominal ganglion of the crayfish Procambarus clarkii. This mean number of neurones with GABA-like immunoreactivity represents approximately 10 % of the total number of neurones in the terminal ganglion. A combination of intracellular staining using Lucifer Yellow and immunocytochemical staining revealed that an identified nonspiking local interneurone (the local directionally selective interneurone, LDS) showed GABA-like immunoreactivity.

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