ABSTRACT
The relationship between the maintenance of the electrical potential difference across frog skin and the oxygen consumption of the skin has been investigated by Mansfield (1910), Lund (1925, 1926, 1928), Lund & Moorman (1931), Francis & Pumphrey (1933), Francis (1933, 1934), Taylor (1935), Boell & Taylor (1933), and Ponder & Macleod (1937). These workers investigated the effects of varying temperatures and varying concentrations of cyanide, oxygen, sulphide, carbon monoxide, butyl alcohol, amyl alcohol, and of various carbamates. The objective of the present work has been to use the somewhat more specific respiratory inhibitor iodoacetate to trace further the oxidative biochemistry of frog skin in so far as it is concerned with the maintenance of the electrical potential difference and to be able to cover all statements made by adequate tests of statistical significance and thereby to eliminate troubles arising from the variability of the skins.
A glass electrode was used for permanganate solutions.
For the full facts as opposed to tentative conclusions see W. L. Francis & O. Gatty, loc at.
Provisional results only; not more than four experiments.
Confirmed by Ponder & Macleod (1937).
The effects of concentration gradients of [HCO3]− across the skin are largely cancelled, however, by having the same bicarbonate rich Ringer on both sides of the skin instead of on one side only.
