ABSTRACT
The effects of temperature upon the adrenergic Na+/H+ exchange of rainbow trout erythrocytes have been studied in vitro. The initial rates of H+ ejection and of increase of intracellular Na+ ([Na+1) in adrenergically stimulated cells were highly temperature-dependent, with apparent Arrhenius activation energies of 112.8±10.0 (mean±S.D., N=4) and 84.1±3.0 kJ mol−1 (N=3), respectively. The steady-state [Na+]i following stimulation decreased progressively with cooling, whilst the time required for [Na+]1 to return to control values after removal of agonist was greatly increased. The change in intracellular pH resulting from adrenergic stimulation was reduced by cooling, such that at 4°C adrenergic responses were barely measurable. The effect of temperature upon the steady state [Na+]i and pHi was probably caused by a disparity in the temperature dependence of the transport mechanisms that contribute to the respective steady states.
