1. Monoclonal antibodies affecting the ligand binding of the neuronal acetylcholine receptor were prepared.

  2. A clone was detected which produced antibodies that increased [125I]-α-bungarotoxin binding, but decreased [3H]acetylcholine binding.

  3. Western blot analysis established that these antibodies did not recognize the receptor protein, but labelled a 20×103Mr polypeptide.

  4. This putative regulatory polypeptide was purified and was found to inhibit [125I]-αr-bungarotoxin binding to pretreated neuronal membranes.

You do not currently have access to this content.