ABSTRACT
Monoclonal antibodies affecting the ligand binding of the neuronal acetylcholine receptor were prepared.
A clone was detected which produced antibodies that increased [125I]-α-bungarotoxin binding, but decreased [3H]acetylcholine binding.
Western blot analysis established that these antibodies did not recognize the receptor protein, but labelled a 20×103Mr polypeptide.
This putative regulatory polypeptide was purified and was found to inhibit [125I]-αr-bungarotoxin binding to pretreated neuronal membranes.
© 1989 by Company of Biologists
1989
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