ABSTRACT
The use of fluorescent dyes for staining neurones has become very common since the introduction of Lucifer Yellow (Stewart, 1978) because it permits high-luminance stainings, for detailed structural analysis, with a minimum of filling time. Lucifer Yellow has been used in a variety of techniques in neurophysiology: in vitro visualization (Stewart, 1978); killing of neurones or parts of neurones (e.g. Miller & Selverston, 1979; Miller & Jacobs, 1984); and in vivo visualization (e.g. Reichert & Krenz, 1986; Kater & Hadley, 1982). We describe a new system for in vivo and in vitro visualization. It can be used with a standard dissecting microscope, features a very simple optical design and costs much less than other systems used previously.
© 1989 by Company of Biologists
1989
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