ABSTRACT
The rate of K+ influx in washed trout erythrocytes was not stable and declined by 60 % over a 6 h period, but decreased only slowly thereafter. During this initial period the cells shrank, although [K+]1 was maintained constant. Both ouabain-sensitive and furosemide-sensitive K+ influx were reduced by approximately equal amounts.
Extensive modification of the saline composition produced no diminution of the loss of K+ influx activity, making it unlikely that the response was due to deficiencies in saline composition.
The rate of K+ influx in unwashed cells was affected only slightly by noradrenaline and adrenaline. By contrast, K+ influx in cells which had been washed and incubated overnight before assay was stimulated 100–250% by these hormones. Half-maximal stimulation was at 2× 10−6 and 8 × 10−6 M respectively. Replacement of plasma by a saline containing 5 × 10−6 M adrenaline and noradrenaline resulted in high and stable K+ influx rates over a 6 h period.
These results support the hypothesis that K+ influx in erythrocytes of freshly drawn blood is high as a result of stimulation by catecholamine hormones. Removal of the normal hormonal milieu, by washing of the cells, results in the rapid loss of K+ influx activity.
Abbreviations: Tris-HCl = [a-amino-a-(nydroxyethyl)propane-1, 3 diol (tris)], MOPS = 3-(N-morpholino) propanesulphonic acid, HEPES = (Af-a-hydroxyethyl)piferazine-iV'-2-ethane3ul phonic acid, ATP = Adenosine-s′-triphosphate.
