...×GFP-op had higher cytotoxicity than 3×MOX-op because it perturbed the ubiquitin-proteasome system. Static aggregates formed by 3×GFP-op dynamically trapped Hsp70 family proteins (Ssa1 and Ssa2 in yeast), causing the heat-shock response. Systematic analysis of mutants deficient in the protein quality...

... in signalling pathways that allow cells to respond to the environment, including nutritional status signalling. TORC1 is widely recognised for its association with various diseases. The budding yeast Saccharomyces cerevisiae has two types of TORC1, Tor1-containing TORC1 and Tor2-containing TORC1, which comprise...

... palmitoyltransferase (SPT). In budding yeast ( Saccharomyces cerevisiae ), SPT is negatively regulated by the two proteins, Orm1 and Orm2. Regulating SPT activity enables cells to adapt SP metabolism to changing environmental conditions. Therefore, the Orm proteins are phosphorylated by two signaling pathways...

...Katherine M. Paine; Kamilla M. E. Laidlaw; Gareth J. O. Evans; Chris MacDonald ABSTRACT The yeast ( Saccharomyces cerevisiae ) plasma membrane (PM) is organised into specific subdomains that regulate surface membrane proteins. Surface transporters actively uptake nutrients in particular regions...

... other functions. Peroxisomal membrane contact sites have obtained little attention compared to those of other cell organelles. However, recent studies resulted in a big leap in our knowledge of the occurrence, composition and function of peroxisomal contact sites. Studies in yeast strongly contributed...

...Ana Cláudia Leite; Telma S. Martins; Rute R. Cesário; Vitor Teixeira; Vítor Costa; Clara Pereira ABSTRACT Cdc28, the homolog of mammalian Cdk1, is a conserved key regulatory kinase for all major cell cycle transitions in yeast. We have found that defects in mitochondrial respiration (including...

... systems could be particularly important during growth transitions, when the CW must rapidly change geometry or mechanics, such as during yeast mating ( Banavar et al., 2018 ), fungal host invasion ( Puerner et al., 2020 ; Ryder et al., 2019 ) or pollen tube navigation in the confined spaces...

... to nutrients such as amino acids and glucose; however, the mechanisms underlying the activation of TORC2 signaling are still poorly understood compared to those for TORC1 signaling. In the budding yeast Saccharomyces cerevisiae , TORC2 targets the protein kinases Ypk1 and Ypk2 (hereafter Ypk1/2), and Pkc1...

..., owing in large part to the challenges associated with accurate determination of cell volume. Much of our comprehension of size regulation derives from yeast models, but even for these morphologically stereotypical cells, assessment of cell volume has mostly relied on proxies and extrapolations from two...

... type I ALG genes Congenital disorders of glycosylation Endoplasmic reticulum Hypoglycosylation Lipid-linked oligosaccharide N -glycosylation Oligosaccharyltransferase Schizosaccharomyces pombe Yeast Agencia Nacional de Promoción Científica y Técnológica http://dx.doi.org...

...Marius Musielak; Carolin C. Sterk; Felix Schubert; Christian Meyer; Achim Paululat; Jürgen J. Heinisch ABSTRACT Rho5 is the yeast homolog of the human small GTPase Rac1. We characterized the genes encoding Rho5 and the subunits of its dimeric activating guanine-nucleotide-exchange factor (GEF...

... sites (so-called heat shock elements or HSEs) for yeast heat shock factor 1 (Hsf1) at 25°C ( Brandman et al., 2012 ). Hsf1 controls expression of genes dedicated to protein folding under both basal and heat-shock conditions ( Solís et al., 2016 ). We observed significant overlaps between the reduced...

... in the plasma membrane of the living cells. We show here that a stable micron-scale protein-depleted region is generated in the plasma membrane of yeast mutants lacking phosphatidylserine at high temperatures. We named this region the ‘void zone’. Transmembrane proteins and certain peripheral membrane proteins...

..., the cell is not completely degraded through autophagy, implying the existence of some kind of termination mechanism. In the yeast Saccharomyces cerevisiae , autophagy is terminated after 10–12 h of nitrogen starvation. In this study, we found that termination is mediated by re-phosphorylation of Atg13...

... find that in Saccharomyces cerevisiae budding yeast, Cdc48 accumulates during cellular stress at intranuclear protein quality control sites (INQ). We show that Cdc48 function is required to suppress INQ formation under non-stress conditions and to promote recovery following genotoxic stress. Cdc48...

... to endocytic cargo. To test this idea, we analyzed the yeast adaptor protein Sla1, which binds membrane proteins harboring the endocytic signal NPFxD via the Sla1 SHD1 domain. Consistently, SHD1 domain point mutations that disrupted NPFxD binding caused a proportional reduction in Sla1–GFP recruitment...

...Fei Wu; Rinse de Boer; Arjen M. Krikken; Arman Akşit; Nicola Bordin; Damien P. Devos; Ida J. van der Klei ABSTRACT The yeast Hansenula polymorpha contains four members of the Pex23 family of peroxins, which characteristically contain a DysF domain. Here we show that all four H. polymorpha Pex23...

...Julian Chollet; Alexander Dünkler; Anne Bäuerle; Laura Vivero-Pol; Medhanie A. Mulaw; Thomas Gronemeyer; Nils Johnsson ABSTRACT Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of septins to this prospective bud site is one of the critical events...

... or mitochondrial matrix, which have nearly identical heme trafficking dynamics, potentially supporting a role for heme as a mitochondrial–nuclear retrograde signal. Moreover, we discovered that the heme synthetic enzyme 5-aminolevulinic acid synthase (ALAS, also known as Hem1 in yeast), and GTPases in control...

.... Nevertheless, when ER stress is prolonged or severe, cell death may occur, accompanied by production of mitochondrial reactive oxygen species (ROS). Using a yeast model ( Saccharomyces cerevisiae ), we describe an innate, adaptive response to ER stress to increase select mitochondrial proteins, O 2 consumption...