...Gillian DeWane; Nicholas M. Cronin; Logan W. Dawson; Christy Heidema; Kris A. DeMali ABSTRACT Vinculin is an actin-binding protein present at cell–matrix and cell–cell adhesions, which plays a critical role in bearing force experienced by cells and dissipating it onto the cytoskeleton. Recently, we...

... in linking mechanical coupling to the actin cytoskeleton and adhesion signaling. Adhesion Signaling Kindlin Rap1 Paxillin RIAM PIP2 Vinculin Actin American Society of Hematology http://dx.doi.org/10.13039/100001422 Pennsylvania Department of Health http://dx.doi.org...

... antibody (pAb) (1:300) was purchased from Sigma-Aldrich. Rabbit-anti-LSP1 pAb (1:100) was purchased from Atlas Antibodies (Bromma, Sweden). Mouse anti-vinculin monoclonal antibody (mAb) (1:200) was purchased from Sigma-Aldrich. Fluorochrome-coupled secondary antibodies (all conjugated with either...

...Naoya Hino; Takafumi Ichikawa; Yasuhisa Kimura; Michiyuki Matsuda; Kazumitsu Ueda; Noriyuki Kioka ABSTRACT Extracellular matrix (ECM) stiffness regulates various cell behaviors, including cell differentiation, proliferation and migration. Vinculin and vinexin α (an isoform encoded by the SORBS3...

... by dephosphorylating vinculin Y822. To specifically probe a role for SHP-2 in E-cadherin mechanotransduction, we mutated vinculin so that it retains its phosphorylation but cannot be dephosphorylated. Cells expressing the mutant vinculin have increased contractility. This work provides a mechanism for inactivating E...

...Poonam Sehgal; Xinyu Kong; Jun Wu; Raimon Sunyer; Xavier Trepat; Deborah Leckband ABSTRACT This study reports novel findings that link E-cadherin (also known as CDH1)-mediated force-transduction signaling to vinculin targeting to intercellular junctions via epidermal growth factor receptor (EGFR...

... Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates that the mechanosensitive focal adhesion (FA) protein vinculin, and both focal adhesion kinase (FAK, also known as PTK2) and Rab5 (both the Rab5a and Rab5b isoforms) have key roles in regulating...

... (FLAP) experiments, we found that the mobility of core FA proteins correlates with their function. Structural proteins such as tensin, talin and vinculin are significantly less mobile in FAs than signaling proteins such as FAK (also known as PTK2) and paxillin. The mobilities of the structural proteins...

... polymerization and retrograde flow of actin, which stabilizes integrins and recruits vinculin to preformed adhesions, particularly those near the leading edge of invasive cells. Rap1 activity also contributes to the tension-induced collective invasive elongation of tumor cell clusters and it enhances tumor cell...

... between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module operating in this tissue. * Author for correspondence ([email protected]) Competing interests The authors declare...

...Aidan P. Maartens; Jutta Wellmann; Emma Wictome; Benjamin Klapholz; Hannah Green; Nicholas H. Brown ABSTRACT Vinculin is a highly conserved protein involved in cell adhesion and mechanotransduction, and both gain and loss of its activity causes defective cell behaviour. Here, we examine how...

..., that phosphorylation of cortactin decreases its interaction with β-catenin and the actin-binding protein vinculin. In addition, binding of vinculin to β-catenin, as well as linkage of vinculin to F-actin, are also significantly compromised upon phosphorylation of cortactin. Accordingly, we found that regulation...

...Vera Auernheimer; Lena A. Lautscham; Maria Leidenberger; Oliver Friedrich; Barbara Kappes; Ben Fabry; Wolfgang H. Goldmann ABSTRACT The focal adhesion protein vinculin connects the actin cytoskeleton, through talin and integrins, with the extracellular matrix. Vinculin consists of a globular head...

... cytoskeleton, Rho-associated protein kinase 1 (ROCK1) and phosphoinositide 3-kinase. VE-cadherin-mediated mechanotransduction triggered local actin and vinculin recruitment, as well as global signals that altered focal adhesions and disrupted peripheral intercellular junctions. Confocal imaging revealed...

... specification of stem cells and affect cancer progression, the molecular mechanisms that sense ECM stiffness have not yet been elucidated. In this study, we show that the proline-rich linker (PRL) region of vinculin and the PRL-region-binding protein vinexin are involved in sensing the stiffness of ECM...

...Alice E. Zemljic-Harpf; Joseph C. Godoy; Oleksandr Platoshyn; Elizabeth K. Asfaw; Anna R. Busija; Andrea A. Domenighetti; Robert S. Ross ABSTRACT Vinculin (Vcl) links actin filaments to integrin- and cadherin-based cellular junctions. Zonula occludens-1 (ZO-1, also known as TJP1) binds connexin-43...

... events that might occur within a key mechanosensitive system at cell–cell junctions; the cadherin–F-actin interface, at which α-catenin and vinculin form a central module. Mechanotransduction at cell–cell junctions emerges as an important signaling mechanism, and we present examples of its potential...

... and negligible adhesive forces were generated. We then asked whether this adhesive area threshold could be modulated by intracellular pathways known to influence either adhesive force, cytoskeletal tension, or the structural link between the two. Expression of talin- or vinculin-head domains that increase...

...Xiao Peng; Laura E. Cuff; Cort D. Lawton; Kris A. DeMali Vinculin was identified as a component of adherens junctions 30 years ago, yet its function there remains elusive. Deletion studies are consistent with the idea that vinculin is important for the organization of cell-cell junctions. However...

... from 0 to 500 kPa. The sequential relationship between Rac1, vinculin adhesion assembly, and replication becomes efficient at above 200 kPa because activation of Rac1 leads to vinculin assembly, actin fiber formation and, subsequently, to initiation of replication. An optimal window of elasticity (200...