... and an NRK-derived cell line expressing v-Src with temperature-sensitive activity, were used to demonstrate that EGF and v-Src stimulation, respectively, induced TC-PTP to colocalize with Cx43 at the plasma membrane. Cell biology experiments using phospho-specific antibodies and biophysical assays...

..., immune evasion and metastasis. To analyze persistent STAT3 activation we coexpressed STAT3 with v-Src. We found that tyrosine phosphorylation of STAT3 by v-Src is independent of Janus kinases (Jaks), the canonical activators of STATs. The STAT3-induced feedback inhibitor, suppressor of cytokine signaling...

...Anna Platek; Marcel Mettlen; Isabelle Camby; Robert Kiss; Mustapha Amyere; Pierre J. Courtoy In Rat-1 fibroblasts, v-Src causes a profound remodelling of cortical actin cytoskeleton. This transformation includes membrane ruffling, a hallmark of the leading edge in migrating cells, and results from...

... by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquitin ligase that controls Src protein levels. These studies also shed light on the role of focal adhesion kinase (FAK) downstream of v-Src...

...V. J. Fincham; A. Chudleigh; M. C. Frame ABSTRACT The v-Src oncoprotein perturbs the dynamic regulation of the cellular cytoskeletal and adhesion network by a mechanism that is poorly understood. Here, we have examined in detail the effects of a temperature-dependent v-Src protein on the regulation...

...Alex Veithen; Mustapha Amyere; Patrick Van Der Smissen; Philippe Cupers; Pierre J. Courtoy ABSTRACT Stable transformation of Rat-1 fibroblasts by the v-Src oncoprotein results into the constitutive formation of macropinosomes. In the present report, we found that macropinosomes do not fuse...

...Alex Veithen; Philippe Cupers; Pierre Baudhuin; Pierre J. Courtoy ABSTRACT The role of v-Src as regulator of fluid-phase pinocytosis was investigated in Rat-1 cells expressing a stable (Rat-1/BB16) or a thermosensitive (Rat-1/tsLA29) v-Src protein. In the second cell line, this protein is inactive...

... in vitro, and also express a temperature-sensitive mutant of the v-Src tyrosine kinase that allows the control of differentiation in a reversible manner. By immunofluorescence and electron microscopy we show that v-Src activity in myotubes leads to an extensive cellular remodeling which affects components...

...Frank Boschelli; Susan M. Uptain; James J. Lightbody ABSTRACT The lethal effects of the expression of the oncogenic protein tyrosine kinase p60 v -src in Saccharomyces cerevisiae are associated with a loss of cell cycle control at the G 1 /S and G 2 /M checkpoints. Results described here indicate...