...Helena L. Palka-Hamblin; Jessica J. Gierut; Wenjun Bie; Patrick M. Brauer; Yu Zheng; John M. Asara; Angela L. Tyner Disruption of the gene encoding protein tyrosine kinase 6 (PTK6) leads to increased growth, impaired enterocyte differentiation and higher levels of nuclear β-catenin in the mouse...

...Camille Faure; Jean-Christophe Corvol; Madeleine Toutant; Emmanuel Valjent; Øivind Hvalby; Vidar Jensen; Said El Messari; Jean-Marc Corsi; Gress Kadaré; Jean-Antoine Girault Proline-rich tyrosine kinase 2 (PYK2) is a non-receptor tyrosine kinase expressed in many cell types and enriched in neurons...

...Aya Takesono; Lisa D. Finkelstein; Pamela L. Schwartzberg The Tec kinases represent the second largest family of mammalian non-receptor tyrosine kinases and are distinguished by the presence of distinct proline-rich regions and pleckstrin homology domains that are required for proper regulation...

... function as GPCR signal transducers. They can form complexes with several signaling proteins,including Src family tyrosine kinases and components of the ERK1/2 and JNK3 MAP kinase cascades. By recruiting these kinases to agonist-occupied GPCRs,β-arrestins confer distinct signaling activities upon...

... pathway may provide a paradigm for understanding how cell adhesion can determine cell cycle progression. © The Company of Biologists Limited 2001 2001 Tyrosine kinase Signal transduction Growth control Cell adhesion Proliferation of mammalian cells is tightly regulated by multiple...

... periods of time (10, 60 or 900 seconds). In some experiments, the cells were pre-incubated for 15 minutes with either the PKC inhibitor GF109203X (2 μM) or the Src family tyrosine kinase inhibitor PP1 (10 μM) before exposure to LTD 4 4. The stimulations were terminated by adding ice-cold lysis buffer (20...

... 4.7 to 3.4. The tyrosine kinase blocker genistein and the protein kinase A inhibitor H-8 reduced the effect of TNFα. A combination of TNFα with interferon-γ acted synergistically on the epithelial barrier. In conclusion, TNFα impairs epithelial barrier function by altering structure and function...

... in vitro, and also express a temperature-sensitive mutant of the v-Src tyrosine kinase that allows the control of differentiation in a reversible manner. By immunofluorescence and electron microscopy we show that v-Src activity in myotubes leads to an extensive cellular remodeling which affects components...

...Giovanna Berruti; Barbara Borgonovo ABSTRACT sp42, a tyrosine kinase of 42 kDa originally found in ejaculated boar spermatozoa, is so far the only tyrosine protein kinase to have been purified from mature male germ cells. We have developed and characterized rabbit polyclonal antibodies specifically...

... by the platelet-derived growth factor and insulin receptors. The effects of lysophosphatidic acid on the Swiss 3T3 actin cytoskeleton can be blocked by the tyrosine kinase inhibitor, tyrphostin. Since tyrphostin does not inhibit the effects of microinjected rho protein, we conclude that lysophosphatidic acid...

...-like domains. The intracellular part of the molecule has a tyrosine kinase domain split by an insertion sequence. Recently, high-affinity cell surface FGF receptors have been identified in various species. We and other groups have also described the isolation of several FGF receptor types in amphibian...

...Lewis C. Cantley; Zhou Songyang ABSTRACT SH2 domains and SH3 domains, found in a number of protein-tyrosine kinases and substrates of protein-tyrosine kinases, provide specificity in downstream signaling. Both of these domains bind to relatively short linear sequences of peptides to provide...

... was phosphorylated on serine and tyrosine residues, suggesting that the activation of protein kinase C also stimulated tyrosine kinase activity. p37 did not precipitate with annexin I or II antibodies. These results show that sheep tracheal cilia contain protein kinase C activity and that activated protein kinase C...

..., and NT-5. These neurotrophin factors interact with two classes of receptors, the trk receptor tyrosine kinase family, and the low affinity p75 neurotrophin receptor. To study potential ligand-receptor interactions, recombinant trk fusion proteins have been constructed, and pan-Zrft polyclonal antisera...

... was investigated by western blot analysis and its tyrosine kinase activity was measured concomitantly. In mononuclear cytotrophoblasts, pp60 c- src was localized at cell-matrix contacts and during the aggregation of cytotrophoblasts, pp60 c- src was distributed on the cell surface at points of cell-cell contact...

... is not sufficient for activation of motility. Activation of motility in vitro by incubation with cyclic AMP can be completely inhibited by a random copolymer of glutamate and tyrosine that inhibits tyrosine kinase activity. Under these conditions, much of the protein phosphorylation associated with activation...

... is associated with tyrosine phosphorylation, the signal transduction pathways and the mechanism by which oncogenic tyrosine kinases induce cell growth and neoplastic changes remain elusive ( Jove and Hanafusa, 1987 ; Yarden and Ullrich, 1988 ). The acutely transforming avian sarcoma viruses have been invaluable...

... tyrosine-phosphorylated proteins tyrosine kinase epididymal maturation Phosphorylation of proteins on tyrosine residues is a rare event in most normal cells ( Hunter & Sefton, 1980 ). However, the finding that biological responses to some growth factors ( Gould et al . 1986 ) and steroid...