...Tomohiro Kubo; Yuma Tani; Haru-Aki Yanagisawa; Masahide Kikkawa; Toshiyuki Oda ABSTRACT α- and β-tubulin have an unstructured glutamate-rich region at their C-terminal tails (CTTs). The function of this region in cilia and flagella is still unclear, except that glutamates in CTTs act as the sites...

... surface. Traditionally, the microtubule has been viewed as simply a track for kinesin motility. New work is challenging this classic view by showing that kinesin-1 and kinesin-4 proteins can induce conformational changes in tubulin subunits while they are stepping. These conformational changes appear...

... microtubule growth and shrinkage. Strikingly, APC-C accumulates at shrinking microtubule extremities, a common characteristic of depolymerases. Cryo-electron microscopy revealed that APC-C adopts an extended conformation along the protofilament crest and showed the presence of ring-like tubulin oligomers...

...Emmanuel T. Nsamba; Mohan L. Gupta, Jr ABSTRACT The microtubule cytoskeleton is assembled from the α- and β-tubulin subunits of the canonical tubulin heterodimer, which polymerizes into microtubules, and a small number of other family members, such as γ-tubulin, with specialized functions. Overall...

... isotope labeling with amino acids in cell culture (SILAC) and RNAseq data. We established specific signatures for OCs and DCs, including genes and proteins of unknown functions. In particular, we showed that OCs and DCs have the same α- and β-tubulin isotype repertoire but that OCs express much more...

... even in non-ciliated cells, we hypothesized that it might be anchored to the centrosome via an interaction with centrosomal proteins. By performing mass spectrometry, we identified several tubulins as potential MICAL-L1 interaction partners, and found a direct interaction between MICAL-L1 and both α...

.../user-licence-1-1/ Summary: Tau governs remodeling of microtubule and actin networks during the tail retraction phase in migrating U87 cells. It also controls the Rho-ROCK signaling via relocation of p190-RhoGAP and FAK proteins. Microtubule-associated protein Tau Tubulin Actin Rho...

... at the right-hand-side interface of the EB microtubule-binding domain with tubulin. Our data are consistent with the model that all three EB paralogues sense the nucleotide state of both β-tubulins flanking their binding site. Their different profile of preferred binding sites contributes to occupying...

... ARL13B is a membrane-associated G-protein that localizes along the cilium and functions in protein transport and signaling. We identify tubulin as a direct interactor of ARL13B and demonstrate that the association occurs via the G-domain and independently from the GTPase activity of ARL13B. The G-domain...

...Montserrat Bosch Grau; Christel Masson; Sudarshan Gadadhar; Cecilia Rocha; Olivia Tort; Patricia Marques Sousa; Sophie Vacher; Ivan Bieche; Carsten Janke ABSTRACT Tubulin is subject to a wide variety of posttranslational modifications, which, as part of the tubulin code, are involved...

.... In mammalian sperm flagella, one of the factors to generate the heterogeneity is tubulin polyglutamylation, although the importance of the heterogeneous modification is unclear. Here, we show that a tubulin polyglutamylase Ttll9 deficiency ( Ttll9 −/− ) causes a unique set of phenotypes related to doublet...

...Marina Serna; Gerardo Carranza; Jaime Martín-Benito; Robert Janowski; Albert Canals; Miquel Coll; Juan Carlos Zabala; José María Valpuesta Tubulin proteostasis is regulated by a group of molecular chaperones termed tubulin cofactors (TBC). Whereas tubulin heterodimer formation is well‐characterized...

... of paclitaxel (PTX), tubulin acetylation induced by trichostatin A (TSA), and the expression of non-acetylatable tubulin were used. With PTX and TSA, FRAP curves best fitted a single phase with a long time constant, whereas with non-acetylatable α-tubulin, curves best fitted a two phase recovery. Upon...

...Sabrina Zink; Lena Grosse; Andrea Freikamp; Sebastian Bänfer; Frauke Müksch; Ralf Jacob Summary The role of post-translational tubulin modifications in the development and maintenance of a polarized epithelium is not well understood. We studied the balance between detyrosinated (detyr...

...Dorota Wloga; Jacek Gaertig Microtubules – polymers of tubulin – perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications...

..., Department of Pharmacology, University of California, Irvine 2226B Gillespie Neuroscience Irvine, CA 92697, USA 15 6 2009 © The Company of Biologists Limited 2009 2009 Centrosome Clathrin Mitosis Plk1 Tubulin GAK Chromosome instability is a key defect of malignant cancer...

...Tonia Akoumianaki; Dimitris Kardassis; Hara Polioudaki; Spyros D. Georgatos; Panayiotis A. Theodoropoulos We have investigated the subcellular distribution and dynamics of soluble tubulin in unperturbed and transfected HeLa cells. Under normal culture conditions, endogenous α/β tubulin is confined...

... structures play key roles. The gene yuri gagarin ( yuri ) encodes a novel protein previously identified through its role in gravitaxis. A male-sterile mutation of yuri has revealed roles for Yuri in the functions of the actin and tubulin structures of spermatogenesis. Yuri is a component of the motile actin...

... by granzyme B. Mass spectrometry analysis revealed that this fragment was the microtubule protein, α-tubulin, which was confirmed by western blotting. In addition, two-dimensional gel analysis showed that the truncated form of α-tubulin had a more basic isoelectric point than the full-length molecule...

... to the tubulin-specific chaperone cofactor E. We find that upon overexpression, E-like depolymerizes microtubules by committing tubulin to proteosomal degradation. Our data suggest that this function is direct and is based on the ability of E-like to disrupt the tubulin heterodimer in vitro. Suppression of E...