.... , Alano P. , Duthie S. , Carter R. (1990). Commitment of the malaria parasite Plasmodium falciparum to sexual and asexual development . Parasitology 100 , 191 - 200 . Crabb B. S. , Cowman A. F. (1996). Characterization of promoters and stable transfection...

... cloned the rat ARHGEF6 gene and investigated, using a transfection approach, its involvement in spine morphogenesis and its relationship to p21-activated kinase 3 (PAK3). We found that expression of tagged ARHGEF6 in hippocampal slice cultures shows a punctate staining in dendritic spines...

.... Recent data indicate that coordinately regulated genes can localise to shared transcription sites. Other transcribed sequences have also been shown to cluster in the nucleus. The ribosomal RNA genes cluster in the nucleoli. Similarly, transiently transfected plasmids and dsDNA viruses form transcription...

..., and expression profiling. Neurochem. Res. 27 , 1065 -1077. Erickson, F. L., Corsa, A. C., Dosé, A. C. and Burnside, B. (2003). Localization of a class III myosin to filopodia tips in transfected HeLa cells requires an actin-binding site in its tail domain. Mol. Biol. Cell 14 , 4173 -4180...

... pathway Transfection The intracellular parasite Plasmodium falciparum is the causative agent of malaria and responsible for over two million deaths each year (Butler, 2002). After an initial multiplication step in liver cells, the parasite invades and multiplies within red blood cells...

... 2003 © The Company of Biologists Limited 2003 2003 RNA-binding protein Chironomus tentans Oligomerization Yeast two-hybrid Transfection Nuclear import In eukaryotic cells, pre-messenger RNA precursors (pre-mRNAs) are cotranscriptionally assembled into ribonucleoprotein...

...Hyun-Hee Kong; Thomas D. Pollard We developed a reliable method for transient transfection of Acanthamoeba using Superfect (Qiagen) and a vector with the Acanthamoeba ubiquitin promoter and enhanced green fluorescent protein (EGFP) as the reporter gene. The transfection efficiency was 3...

... on a surface loop in the mature domain of all the CPBs characterised to date. The CPB2.8 glycosylation site (N103) was mutated to a serine (N103S) in order to address any potential role that this motif may have in trafficking of the enzymes. Following transfection of Δ CPB with pGL48, immunolocalisation...

... binding and effects on the cytoskeleton? We have shown previously (Gimona and Mital, 1998) that h 2 CaP binds to actin in in vitro co-sedimentation assays and co-localizes with F-actin in transfected fibroblasts, despite the absence of the consensus actin-binding motif in the region 142-163 (ABS1). We...

... by polyethylenimine-adenofection. Expression studies were also carried out using the non-neuronal cell line COS-7. Immunofluorescence microscopy was performed using wild-type isoforms and an α mutant subunit bearing the gephyrin-binding motif of the β subunit. In COS-7 cells, transfected glycine receptor α subunits...

..., beyond the repeats, increased actin binding, suggesting that the carboxy-terminal tail may modulate actin association. Results obtained from transient transfections of amino- and carboxy-terminal truncations in h 1 calponin were consistent with the established location of the actin binding motif outside...

... microfilaments or membrane associated structures (Fig. 1D,F). It remains possible that some fine structures in the cytoplasm could be masked by the high fluorescence signal caused by a large amount of injected protein. However, expression of lower levels of the protein in transfected cells also revealed...

... transient transfection of primary rat cortical neurons, BDNF-GFP was found in secretory granules of the regulated pathway of secretion, as indicated by colocalization with the secretory granule marker secretogranin II. BDNF-GFP vesicles were found in the neurites of transfected neurons with a pattern...

... occludin. This was accomplished by first transfecting MDCK cells with the Lac repressor gene. Hyg R clones were then transfected with chick occludin cDNA inserted into a Lac operator construct. The resulting Hyg R /Neo R clones were plated on porous inserts and allowed to form tight junctions. Once steady...

... hormone (αMSH). From several stable transfectant cell lines expressing the human MC1 receptor in relatively high numbers, three melanin producing clones (G4F-12, 14, and 15) and one amelanotic clone (G4F-7) were further analyzed in competition binding experiments and in cAMP and melanin assays...

... analyzed the effects of MAP2c and MAP2d expression on microtubule and microfilament organization in transiently transfected cells. Our results show that both variants stabilize microtubules, but only MAP2d stabilizes microfilaments. * Author for correspondence 09 10 1995 01 01 1996 ©...

... 1995 by Company of Biologists 1995 myosin I localization subcellular fractionation transfection Semliki Forest Virus membrane It has become evident that many cells express a number of different myosins. Myosins share a characteristic head or motor domain that can bind F-actin...

... in non-neuronal cells by transfection MAP2 stabilises microtubules and induces their rearrangement into long bundles that are capable of supporting process outgrowth. To investigate which elements in the MAP2 sequence are involved in these functions we have constructed a series of deletion mutants...

... the adhesive properties of epican apart from the influence of other adhesive molecules found on keratinocytes, mouse L cell fibroblasts were transfected with CD44Epican cDNA. The epican expressed on the surface of transfected L cells was predominantly a heparan or chondroitin sulfate proteoglycan...

... into bundles. Transfection of a villin-specific cDNA into cultured cells that do not produce this protein results in the growth of long surface microvilli and the reorganization of the underlying actin cytoskeleton. Here we studied the effects of low concentrations of cytochalasin D on the induction...