...Simon Ullrich; Iliya Nadelson; Stefan Krebs; Helmut Blum; Heinrich Leonhardt; Irina Solovei ABSTRACT Transcription of the majority of eukaryotic genes is accompanied by splicing. The timing of splicing varies significantly between introns, transcripts, genes and species. Although quick co...

... role in the regulation of gene expression. Broadly speaking, chromatin can be classified as either being in a more accessible and transcriptionally active state (euchromatin or ‘A’ compartment) or a condensed, transcriptional inactive state (heterochromatin or ‘B’ compartment) (see Glossary...

...-transcriptionally after polyadenylation ( Choquet et al., 2023 ). Accordingly, a recent imaging study has found that incompletely processed pre-mRNAs undergo splicing as they slowly move through a zone proximal to the transcription site ( Coté et al., 2024 ). Early studies visualizing spliceosome components...

...Zhi Yang Tan; Shujun Cai; Saayli A. Paithankar; Tingsheng Liu; Xin Nie; Jian Shi; Lu Gan When starved of nitrogen, fission yeast Schizosaccharomyces pombe cells enter a quiescent “G0” state with smaller nuclei and transcriptional repression. The genomics of S. pombe G0 cells has been well studied...

... resulted in decreased luciferase activity of the minimal promoter ( Fig. 3C,D ). Dicer expression is also regulated post-transcriptionally, as a number of alternative splicing variants have been identified for the Dicer transcript, with some encoding shorter Dicer isoforms and others not encoding any...

...Bina Prajapati; Maria Sokolova; Ekaterina Sidorenko; Mikael Kyriacou; Salla Kyheröinen; Anniina Vihervaara; Maria K. Vartiainen ABSTRACT Myocardin-related transcription factors (MRTFs) are coactivators of serum response factor (SRF), and thereby regulate cytoskeletal gene expression in response...

... clustering of the AR for the formation of transcriptionally active condensate-like droplets via the assembly of highly dynamic actin polymers. Together, these examples highlight that dynamic nuclear actin polymerization regulates gene transcription in response to different signaling cues by modulating...

... the antiproliferative effect of FGF2 modulates chromatin-, nucleolus- and rDNA-associated proteins. The chromatin and nucleolar proteome indicated that FGF2 stimulation modulates proteins related to transcription, rRNA expression and chromatin-remodeling proteins. The global transcriptional rate and nucleolus area...

... fine tune the induction of autophagy without complete abrogation. In this Review, we summarize the recent discoveries on the mechanism of regulation of ATG (autophagy related) gene expression at the level of transcription, post transcription and translation. Furthermore, we briefly discuss the role...

... transcriptional partners. However, the network of factors that controls SMAD2 chromatin binding and shapes its transcriptional programme over time is poorly characterised. Here, we combine ATAC-seq with computational footprinting to identify temporal changes in chromatin accessibility and transcription factor...

... to wild-type data, uncoupling Hi-C-measured long-range DNA contacts from transcriptional repression of the X chromosomes. Taken together, our results indicate that DPY-21 has a non-catalytic role in regulating the dynamics of condensin DC binding, which is important for transcription repression...

... by The Company of Biologists Ltd 2022 Summary: The TAZ protein, an effector of Hippo signaling that phase separates into biomolecular condensates in the nucleus, is shown to hold myogenic cells in an undifferentiated state by repressing a muscle-specific transcription complex. WW domain-containing...

...David Z. Kochan; Julia S. P. Mawer; Jennifer Massen; Kiril Tishinov; Swati Parekh; Martin Graef; Anne Spang; Peter Tessarz ABSTRACT Gene expression involves regulation of chromatin structure and transcription, as well as processing of the transcribed mRNA. While there are feedback mechanisms...

... including protein biosynthesis and transcription, and dedicated follow-up experiments highlight the importance of accurate transcriptional and translational control for mitigating protein aggregation and toxicity during arsenite stress. Some of the hits are associated with pathological conditions...

... early in infection remained unexplained. We thus assessed the amount of transcriptionally active nuclear vDNA. Cellular gene loci active in transcription have been visualized using RNA FISH targeting intron sequences ( Levesque and Raj, 2013 ; Shah et al., 2018 ), or by monitoring transcriptional...

..., suggests that MVI is present in a number of transcriptionally active areas in the nucleus. Furthermore, given the interaction of MVI with both the estrogen receptor ( Fili et al., 2017 ) and the androgen receptor ( Loikkanen et al., 2009 ), we speculate that MVI is required for the transcription...

.... However, the molecular mechanisms that regulate nuclear structure–function relationships during heat shock remain unclear. Here, we show that lamin A and C (hereafter lamin A/C; both lamin A and C are encoded by LMNA ) are required for heat-shock-mediated transcriptional induction of the Hsp70 gene locus...

... of genes, whose functions include regulation of the cell cycle, transcription and metabolism ( Mahat et al., 2016 ; Vihervaara et al., 2017 ). In vertebrates, the HSR is transcriptionally regulated by a family of heat-shock factors (HSFs) comprising several members, namely, HSF1, HSF2, HSF3, HSF4...

... and in cells. Transient interactions detected through BioID link actin to several steps of transcription as well as to RNA processing. Alterations in nuclear actin levels disturb alternative splicing in minigene assays, likely by affecting the transcription elongation rate. This interactome analysis thus...

... associates differently with RNA polymerase II; a gene reporter assay revealed that HP1a and HP1b, but not HP1c, inhibit transcriptional activity, suggesting that HP1c serves as a positive regulator in transcription. Thus, these studies provide the basic clues pertaining to the molecular mechanism by which...