... Tip growth Vesicle transport National Science Foundation (NSF) CBET 1309933 NSF-MCB 1253444 National Institutes of Health (NIH) R01GM121679 Polarized cell growth, a mechanism by which plasma membrane and cell wall material are deposited to a defined region...

... plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation. The construct continued to mark the PM at the root hair apical dome, but only during periods of reduced growth. During rapid tip growth, AGD1-GFP labeled the PM of the lateral flanks...

...Jae-Ung Hwang; Guang Wu; An Yan; Yong-Jik Lee; Claire S. Grierson; Zhenbiao Yang Rapid tip growth allows for efficient development of highly elongated cells (e.g. neuronal axons, fungal hyphae and pollen tubes) and requires an elaborate spatiotemporal regulation of the growing region. Here, we use...

...R. M. Parton; S. Fischer-Parton; M. K. Watahiki; A. J. Trewavas Regulated secretory vesicle delivery, vesicle fusion and rapid membrane recycling are all contentious issues with respect to tip growth in plant, fungal and animal cells. To examine the organisation and dynamics of membrane movements...

... in accumulation of Lucifer Yellow in their vacuoles, which were smaller than normal and often misplaced. The dikaryotic hyphae, produced by the fusion of two kin2 -null sporidia, showed tip growth, but unlike wild-type hyphae, these structures lacked the large, basal vacuole and contain significantly more 200–400...

... was disrupted with latrunculin, and increased isometrically in size rather than producing germ tubes. Therefore, F-actin participates in initiating tip formation in addition to its previously demonstrated participation in maintenance of hyphal tip growth. The cortical location and radial organization...

...R. M. Parton; S. Fischer; R. Malhó; O. Papasouliotis; T. C. Jelitto; T. Leonard; N. D. Read ABSTRACT The existence of pronounced cytoplasmic pH gradients within the apices of tip-growing cells, and the role of cyto-plasmic pH in regulating tip growth, were investigated in three different cell types...

... the rates of tip growth without changing tip morphology, thus indicating that the channels are not absolutely essential for tip growth. Furthermore, in contrast to the hyphae of another tip growing organism, Saprolegnia ferax , tip-high gradients of neither spontaneous nor stretch activated channels were...

... in the hyphal tip plasma membrane of the oomycete Saprolegnia ferax . J. Cell Sci . 101 , 721 – 730 . 10.1242/jcs.101.3.721 Garrill , A. , Jackson , S. L. , Lew , R. R. and Heath , I. B. ( 1993 ). Ion channel activity and tip growth: tip-localized stretch-activated channels...

...Natalia N. Levina; Roger R. Lew; I. Brent Heath ABSTRACT Growing hyphal tips of the oomycete Saprolegnia ferax possess a tip-high gradient of stretch-activated ion channels permeable to calcium. These mechanosensitive channels appear to play a direct role in the polarized tip growth process...

... fungi contractile vacuoles tip growth oomycetes Differentiation during animal development involves many cellular shape changes mediated by both the cytoskeleton and selective cell-substrate interactions. In contrast, in plants and fungi many aspects of differentiation are mediated by selected...

...Ashley Garrill; Roger R. Lew; I. Brent Heath ABSTRACT We have used the patch clamp technique to study ion channels involved in hyphal tip growth in the oomycete Saprolegnia ferax . The development of a technique in which protoplasts are produced in distinct linear arrays has permitted the study...

... of actively growing Lilium pollen tubes that declines to a uniform basal level of ∼170 nM throughout the length of the tube. The calcium gradient occurs within the first 10–20 μM proximal to the tip. Experimental inhibition of tip growth, usually achieved through the injection of the Ca 2+ buffer 5,5’-dibromo...

... preprophase band tip growth Plant cell walls prevent cell migration in most tissue and therefore the control of the position and the orientation of new cell plates are prerequisites for normal plant morphogenesis. The division site, the position where a cell plate fuses to the parental cell wall...

... from the tip, except for the obvious absence of nuclear associations in the nuclear-free zone. Fig. 7 summarizes, in semi-quantitative scale-diagrammatic form, a synthesis of all of the above results. One of the most enigmatic questions in tip-growth systems is the mechanism responsible...

...J. H. Doonan; D. J. Cove; C. W. Lloyd ABSTRACT In this study we compare the contributions of Factin and microtubules to tip growth in filamentous cells of the moss Physcomitrella patens . In tip growth, expansion seems to be restricted to the hemispherical apical dome. Cytoskeletal elements have...

... cells with CIPC - a drug that is known to produce multiple microtubuleorganizing centres — and which here produces multiple foci for microtubules at the tip. These observations emphasize the involvement of microtubules in tip growth, alignment of the cell plate and nuclear migration - processes...

... to be generated during tip growth. A spiralling of microtubules was seen at the apices of onion root hairs. Using shadow-cast preparations of macerated radish root hairs, it was confirmed that steeply helical microtubules matched the texture of the inner wall. In onion, the 45° microtubular helices...