...Raven J. Peterson; Ryan C. Reed; Colin R. Zamecnik; Marwa A. Sallam; Joel A. Finbloom; Francisco J. Martinez; Joshua M. Levy; Aekkacha Moonwiriyakit; Tejal A. Desai; Michael Koval ABSTRACT Tight junctions regulate epithelial barrier function and have been shown to be influenced by multiple classes...

..., to address the role of PALS1 at the cellular level, we generated CRISPR/Cas9-mediated PALS1-knockout MDCKII cell lines. The loss of PALS1 resulted in increased paracellular permeability, indicating an epithelial barrier defect. This defect was associated with a redistribution of several tight junction...

...Silvio Holzner; Sophie Bromberger; Judith Wenzina; Karin Neumüller; Tina-Maria Holper; Peter Petzelbauer; Wolfgang Bauer; Benedikt Weber; Klaudia Schossleitner ABSTRACT Dysfunction of vascular barriers is a critical step in inflammatory diseases. Endothelial tight junctions (TJs) control barrier...

...Saranyaraajan Varadarajan; Rachel E. Stephenson; Ann L. Miller ABSTRACT Epithelial cells form tissues that generate biological barriers in the body. Tight junctions (TJs) are responsible for maintaining a selectively permeable seal between epithelial cells, but little is known about how TJs...

...Brian Belardi; Sungmin Son; Michael D. Vahey; Jinzhi Wang; Jianghui Hou; Daniel A. Fletcher ABSTRACT Tight junctions have been hypothesized to act as molecular fences in the plasma membrane of epithelial cells, helping to form differentiated apical and basolateral domains. While this fence function...

...Bhargavi Manda; Hina Mir; Ruchika Gangwar; Avtar S. Meena; Shrunali Amin; Pradeep K. Shukla; Kesha Dalal; Takuya Suzuki; RadhaKrishna Rao ABSTRACT The apical junctional complex (AJC), which includes tight junctions (TJs) and adherens junctions (AJs), determines the epithelial polarity, cell-cell...

..., epithelia at mucosal surfaces must allow selective paracellular flux that discriminates between solutes and water while preventing the passage of bacteria and toxins. In vertebrates, tight junctions seal the paracellular space; flux across the tight junction can occur through two distinct routes that differ...

..., and validated as a so-far-unknown EphA2-interacting protein. Loss of EphA2 protein disrupted the subcellular distribution of afadin and occludin in differentiated keratinocytes, leading to impairment of tight junctions. Collectively, these studies illustrate the use of the BioID approach in order to map...

... of the myelinated axon and the vertebrate epithelial tight junction. These more-recent structures appear to have evolved through alterations of the ancestral septate junction. During its formation in the developing animal, the pSJ exhibits plasticity, although the final structure is extremely robust. Similar...

.... Highlighted Article: Here we develop a high-definition imaging approach to dissect and assign function to proteins involved in the rapid process of malaria parasite invasion of the human erythrocyte. Plasmodium falciparum Merozoite Erythrocyte invasion Tight junction Deconvolution...

.... Published by The Company of Biologists Ltd 2015 Highlighted Article: The epithelial cholinergic system activates a signaling pathway that regulates paracellular permeability by modulating the content and distribution of the tight junction protein claudin-4. Tight junction Claudin-4...

... of tight junctions (TJs) has not been explored. We hypothesized that IQGAP1 could regulate TJ formation by modulating the expression and/or localization of junctional proteins, and we systematically tested this hypothesis in the model Madin-Darby canine kidney (MDCK) cell line. We find that IQGAP1...

... abolishing Akt phosphorylation, which led to an induction of MMP-9. This increase in MMP-9 secretion perturbed the Sertoli cell tight junction permeability barrier by proteolysis-mediated downregulation of tight junction proteins at the BTB. These findings were confirmed by the use of a specific MMP-9...

...Ceniz Zihni; Maria S. Balda; Karl Matter ABSTRACT Tight junctions are a component of the epithelial junctional complex, and they form the paracellular diffusion barrier that enables epithelial cells to create cellular sheets that separate compartments with different compositions. The assembly...

... differentiation. Because extracellular Ca 2+ is essential for the development of stable epithelial tight junctions (TJs), we hypothesized that the CaSR participates in regulating TJ assembly. We first assessed the expression of the CaSR in Madin-Darby canine kidney (MDCK) cells at steady state and following...

...Jessica L. Maiers; Xiao Peng; Alan S. Fanning; Kris A. DeMali Summary The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent...

...Laurel S. Rodgers; M. Tanner Beam; James M. Anderson; Alan S. Fanning Summary Tight junctions (TJs) regulate the paracellular movement of ions, macromolecules and immune cells across epithelia. Zonula occludens (ZO)-1 is a multi-domain polypeptide required for the assembly of TJs. MDCK II cells...

...Xin Cong; Yan Zhang; Ning-Yan Yang; Jing Li; Chong Ding; Qian-Wen Ding; Yun-Chao Su; Mei Mei; Xiao-Hong Guo; Li-Ling Wu; Guang-Yan Yu Summary Occludin plays an important role in maintaining tight junction barrier function in many types of epithelia. We previously reported that activation...

...Jimmi Cording; Johanna Berg; Nadja Käding; Christian Bellmann; Christian Tscheik; Julie K. Westphal; Susanne Milatz; Dorothee Günzel; Hartwig Wolburg; Jörg Piontek; Otmar Huber; Ingolf Ernst Blasig Summary Tight junctions seal the paracellular cleft of epithelia and endothelia, form vital barriers...

... integrity. Initial experiments showed ICAM-1 to co-immunoprecipitate and co-localize with tight junction and basal ectoplasmic specialization proteins such as occludin and N-cadherin, which contribute to BTB function. More importantly, overexpression of ICAM-1 in Sertoli cells in vitro enhanced barrier...