... transport. Rab11 is the key factor regulating the post-Golgi transport of rhodopsin 1 (Rh1; also known as NinaE), a photoreceptive protein, to the rhabdomere, a photoreceptive plasma membrane. Here, we found that neuronal Synaptobrevin (nSyb) colocalizes with Rab11 on the trans-side of Golgi stacks and post...

...Elena Fdez; Mar Martínez-Salvador; Matthew Beard; Philip Woodman; Sabine Hilfiker Neurosecretion involves fusion of vesicles with the plasma membrane. Such membrane fusion is mediated by the SNARE complex, which is composed of the vesicle-associated protein synaptobrevin (VAMP2), and the plasma...

...Helmut Kubista; Hannah Edelbauer; Stefan Boehm The core complex, formed by the SNARE proteins synaptobrevin 2, syntaxin 1 and SNAP-25, is an important component of the synaptic fusion machinery and shows remarkable in vitro stability, as exemplified by its SDS-resistance. In western blots...

...Gary W. Lawrence; J. Oliver Dolly Ca 2+ -triggered catecholamine exocytosis from chromaffin cells involves SNAP-25, synaptobrevin and syntaxin (known as SNAREs). Synaptotagmin I has been implicated as the Ca 2+ -sensor because it binds Ca 2+ , and this enhances its binding to syntaxin, SNAP-25...

... and in which SNAP-25 and syntaxin predominate over synaptobrevin. Their formation increased upon Ca 2+ -stimulated exocytosis; notably, the 2D protocol proved much superior to 1D SDS-PAGE for the detection of large complexes and revealed that for forms with relative molecular mass greater than 100,000...

...P. Taubenblatt; J. C. Dedieu; T. Gulik-Krzywicki; N. Morel ABSTRACT Synaptic vesicle docking and exocytosis require the specific interaction of synaptic vesicle proteins (such as VAMP/synaptobrevin) with presynaptic plasma membrane proteins (such as syntaxin and SNAP 25). These proteins form...

...Julia Avery; Alois Hodel; Michael Whitaker ABSTRACT Sea urchin eggs provide an efficient in vitro model of exocytosis. We have identified proteins in sea urchin eggs that cross-react with antibodies to mammalian synaptobrevin, synaptotagmin, SNAP-25, syntaxin and rab3a. We show that these proteins...

... are Zn 2+ -dependent proteases that cleave VAMP/synaptobrevin and SNAP-25, two proteins which can form a ternary complex (termed the SNARE complex) with syntaxin and have been implicated in the docking of synaptic vesicles at the plasma membrane. The onset of toxin-induced inhibition of neurotransmitter...

... not present in the transcytotic and storage vesicle proteins. Using mutagenesis we have distinguished the signals for rapid endocytosis and SV targeting in synaptobrevin. Finally, we have evidence that synaptic vesicles can be generated from an endosomal compartment in vitro. © The Company of Biologists...