... functions, including providing structural support to cells, as well as active roles in mechanical support and signaling pathways. Consequently, defects in IFs are associated with more than 100 diseases. In this Cell Science at a Glance article, we discuss the established classes of IFs and their general...

... of the biology and genetics of LRRK2 make it a fascinating and challenging subject for investigation. The past few decades since the first functional analyses of LRRK2 have witnessed substantial advances in our understanding of the genetics, structure, function and role of this protein. Even in the past five...

... it is essential during development, FAK also drives invasive cancer progression and metastasis. On a structural level, the basic building blocks of FAK have been described for some time. However, a picture of how FAK integrates into larger assemblies in various cellular environments, including one of its main...

... of dynamin in vivo depends on its oligomerization and its capacity for efficient GTP hydrolysis, the molecular mechanisms governing these activities have remained poorly defined. In recent years, there has been an explosion of structural data that has provided new insights into the architecture, organization...

... and chemical signals that are controlled by integrins are essential for intercellular communication and underpin all aspects of metazoan existence. To mediate such diverse functions, integrins exhibit structural diversity, flexibility and dynamism. Conformational changes, as opposed to surface expression...

... 25 7 2003 © The Company of Biologists Limited 2003 2003 Golgi Endosomes Cholesterol Raft Structure p24 proteins Proteins of the p24 family form a rather unique family of abundant, small (20-24 kDa) type I trans-membrane proteins in the early biosynthetic pathway. They can...

...Amit Mehta Recent experiments, drawing upon single-molecule, solution kinetic and structural techniques, have clarified our mechanistic understanding of class V myosins. The findings of the past two years can be summarized as follows: (1) Myosin V is a highly efficient processive motor, surpassing...

...Folma Buss; Helen Kent; Murray Stewart; Susanne M. Bailer; John A. Hanover ABSTRACT We have expressed rat nucleoporin p62 cDNA in Escherichia coli to obtain material for structural and self-association studies. Electron microscopy and circular dichroism spectroscopy are consistent with a rod-shaped...

...Robert W. Kensler; Murray Stewart ABSTRACT Rabbit muscle is a major source of material for biochemical experiments and spin labelling studies of contraction, and so it is important to establish how closely this material resembles the frog and fish muscles usually used for structural studies...

... molecular interactions structure Lamins are the principal components of the fibrous lamina that underlies the nuclear envelope of eukaryotic cells (reviewed by Gerace, 1986 ). The lamina fibres are about 10 nm in diameter and are arranged in a dense fibrous mat that sometimes shows a remarkable...

... of expressed LMM cDNA indicated that charge zone complementation is not a major driving force in myosin polymerisation. Instead, it probably serves to prevent unfavourable interaction geometries. © The Company of Biologists Limited 1991 1991 myosin assembly structure mutagenesis expression...

...Murray Stewart; Sue Whytock; Robert D. Moir ABSTRACT We have combined structural, biochemical and recombinant DNA methods to explore molecular interactions involved in nuclear envelope assembly dynamics and nucleocytoplasmic transport. Electron microscopy has established the overall architecture...

...Sue Whytock; Robert D. Moir; Murray Stewart ABSTRACT We have used enzymic digestion as a structural probe to investigate components of the nuclear envelope of germinal vesicles from Xenopus oocytes. Previous studies have shown that these envelopes are composed of a double membrane in which nuclear...

...N. Q. Mcdonald; R. Lapatto; J. Murray-Rust; T. L. Blundell ABSTRACT The largest and best characterised family of neurotrophic growth factors is that of nerve growth factor (NGF) and its relatives. In order to understand the relation of structure and function, we have undertaken X-ray analyses...