...Jiaoyue Long; Guoning Liao; Yinna Wang; Dale D. Tang ABSTRACT The tyrosine kinase c-Abl participates in the regulation of various cellular functions including cell proliferation, adhesion, migration, smooth muscle contraction and cancer progression. However, knowledge regarding transcriptional...

... Ca 2+ release often begins at a single site, then regeneratively propagates through the cell as a Ca 2+ wave. The Ca 2+ wave consistently begins at the same site on successive activations. Here, we address the mechanisms that determine the Ca 2+ wave initiation site in intestinal smooth muscle cells...

...D. MacMillan; C. Kennedy; J. G. McCarron Summary Adenosine 5′-triphosphate (ATP) mediates a variety of biological functions following nerve-evoked release, via activation of either G-protein-coupled P2Y- or ligand-gated P2X receptors. In smooth muscle, ATP, acting via P2Y receptors (P2YR), may act...

...Brant E. Isakson Inositol (1,4,5)-trisphosphate [Ins(1,4,5) P 3 ] originating in the vascular smooth-muscle cells (VSMCs) has been shown to modulate the Ca 2+ stores in endothelial cells (ECs). However, the reverse is not found, suggesting that Ins(1,4,5) P 3 movement might be unidirectional across...

...John G. McCarron; Susan Chalmers; Thomas C. Muir Smooth muscle responds to activation of the inositol (1,4,5)-trisphosphate receptor [Ins(1,4,5) P 3 R] with a graded concentration-dependent (`quantal') Ca 2+ release from the sarcoplasmic reticulum (SR) store. Graded release seems incompatible both...

... m was examined during Ca 2+ oscillations in smooth muscle cells. In single, voltage-clamped smooth muscle cells, inhibition of mitochondrial Ca 2+ accumulation inhibited inositol (1,4,5)-trisphosphate [Ins(1,4,5) P 3 ]-evoked Ca 2+ release and prolonged the time required for restoration of [Ca 2...

...Nicolas Fritz; Jean-Luc Morel; Loice H. Jeyakumar; Sidney Fleischer; Paul D. Allen; Jean Mironneau; Nathalie Macrez Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1 -null...

... that potentiates Ca 2+ release from the IP 3 R in smooth muscle. The presence of FKBP12 was confirmed in colonic myocytes and co-immunoprecipitated with the IP 3 R. In aortic smooth muscle, however, although present, FKBP12 did not co-immunoprecipitate with IP 3 R. In voltage-clamped single colonic myocytes...

... the hypothesis that MARCKS is a regulator of the spatial distribution of CaM within the cytoplasm of differentiated smooth-muscle cells. In overlay assays with portal-vein homogenates, CaM binds predominantly to the MARCKS-containing band. MARCKS is abundant in portal-vein smooth muscle (∼16 μM) in comparison...

... The Company of Biologists Limited 2005 2005 Cyclic ADP-ribose Ryanodine receptors Ca 2+ oscillations Acetylcholine Smooth muscle Ca 2+ signalling is the most `universal and versatile' signal transduction mechanism, mediating the intracellular effects of environmental signals into specific...

...Gerald Burgstaller; Mario Gimona Activation of conventional protein kinase C by phorbol ester triggers the Src-dependent remodelling of the actin cytoskeleton and the formation of podosomes in vascular smooth muscle cells. Rearrangement of actin cytoskeleton in response to phorbol-12,13-dibutyrate...

...Irina Kaverina; Theresia E. B. Stradal; Mario Gimona Phorbol ester triggers the conversion of focal adhesions into podosomes in A7r5 smooth muscle cells. Here we followed the dynamics of podosome formation using dual fluorescence live video and confocal microscopy, as well as interference...

...Arnaud Scherberich; Manuel Campos-Toimil; Philippe Rondé; Kenneth Takeda; Alain Beretz ABSTRACT Migration of vascular smooth muscle cells (VSMC) is a key event in the formation of neointima during atherosclerosis. Fura-2 loaded VSMCs were used to investigate calcium homeostasis during cell...

...Lisa M. Dahm; Chauncey W. Bowers ABSTRACT Previous work from this laboratory has established a method for maintaining physiological contractility of dissociated avian smooth muscle in a defined medium at low density. The present report emphasizes the dramatic potency of serum to alter smooth muscle...

... of smooth muscle, including vascular, gastrointestinal and genitourinary smooth muscles. In addition, α7 was surface-expressed by a number of smooth muscle cell lines that maintained their differentiated phenotype following adaptation to culture. Studies with the mouse 9E11G smooth muscle cell line showed...

...Asako Goi Terasaki; Hiroyuki Nakagawa; Eiji Kotani; Hajime Mori; Kazuyo Ohashi ABSTRACT We purified a 450 kDa protein from a low-salt alkaline extract of chicken gizzard smooth muscle. This high molecular mass protein could be extracted with the low-salt alkaline solution at 37°C but not at 4°C...

...-α 8 polyclonal antibody in adult rat tissues shows prominent staining in vascular and visceral smooth muscle. In addition, the antibody strongly stained kidney mesangial cells and a cell type in the alveolar wall of the lungs, most likely corresponding to alveolar myofibroblasts. These results...

...D. Proudfoot; D. P. Parrott; D. E. Bowyer ABSTRACT P388D1 macrophage-like cells have previously been shown to produce both mitogenic and inhibitory regulators of porcine smooth muscle cell (pSMC) growth. The mitogenic activity was shown to have a molecular mass of >10 kDa while the inhibitory...

...Alison J. North; Mario Gimona; Robert A. Cross; J. Victor Small ABSTRACT Calponin and caldesmon are two thin filament-binding proteins found in smooth muscle that have both been attributed a role in modulating the interaction of actin and myosin. Using high-resolution dual-label immunocyto...

...Alison J. North; Mario Gimona; Zeev Lando; J. Victor Small ABSTRACT Differentiated smooth muscle cells typically contain a mixture of muscle (α and γ) and cytoplasmic (β and γ) actin isoforms. Of the cytoplasmic actins the β-isoform is the more dominant, making up from 10% to 30% of the total actin...