... and the Syt1-Sec7 domain promoted nucleotide exchange on Arl1p in vitro. Syt1p function required the N-terminal region, Sec7 and PH domains. Arl1p, but not Arl3p, interacted with Syt1p. Localization of Syt1p to the Golgi did not require Arl3p. Unlike arl1 Δ or arl3 Δ mutants, syt1 Δ did not show defects...

... they recruit specific structural proteins such as Golgins. Yeast Arl1p directly interacts with Mon2p/Ysl2p, a protein that displays some sequence homology to the large Sec7 guanine exchange factors (GEFs) of Arf1. Mon2p also binds the putative aminophospholipid translocase (APT) Neo1p, which performs essential...

... for correspondence 21 06 1993 28 07 1993 © 1993 by Company of Biologists 1993 Golgi immunoisolation Kex2p sec7 yeast The Golgi apparatus plays a central role in the endomem-brane system of eukaryotic cells ( Farquhar, 1985 ; Mellman and Simons, 1992 ). In mammalian cells...