..., and visceral endoderm (VE) that contacts the developing epiblast and extraembryonic ectoderm. Mouse extraembryonic endoderm (XEN) cells were isolated and became widely used to study signals governing lineage specification. Rat XEN cell lines have also been derived, but were distinguished from mouse...

... male Sprague Dawley rats (2-months old) housed in a 12-hour light-dark cycle animal facility received a single injection of NMDA (100 mg/kg) or the non-competitive NMDA receptor antagonist MK801 (5 mg/kg) intraperitoneally in 0.9% saline. To measure cell proliferation, animals were injected...

... membrane is not sufficient to trigger exocytotic membrane fusion. A mast cell line, rat basophilic leukemia cell (RBL-2H3), was cultured in Eagle's minimal essential medium from Nissui (Tokyo, Japan) with 10% fetal calf serum (Boehringer Mannheim) at 37°C in an atmosphere of 5% CO 2 . Mastocytoma P815...

... of insulin sensitivity and adipocyte differentiation. However, additional metabolic or endocrine functions of this molecule remain largely unexplored. In this study, a series of experiments were undertaken to explore the potential expression, regulation and functional role of this novel adipocytokine in rat...

... activity, which was shown previously to be expressed specifically by strongly metastatic cells, controls galvanotaxis. Two well-defined rat (`Dunning') cell lines, originally derived from the same prostate tumour but differing markedly in their metastatic ability, were used. Cells were exposed to exogenous...

...Kevin M. McCarthy; Michael Lam; Laura Subramanian; Reena Shakya; Zhen Wu; Estelle E. Newton; Neil E. Simister ABSTRACT The neonatal Fc receptor, FcRn, transports immunoglobulin G (IgG) across intestinal epithelial cells of suckling rats and mice from the lumenal surface to the serosal surface...

... the intracellular turnover of immune complexes after Fcγ receptor mediated endocytosis in cultured rat liver endothelial cells and Kupffer cells by means of isopycnic centrifugation, DAB cross-linking and morphological techniques. For the biochemical experiments the antigen, dinitrophenylated bovine serum albumin...

... composed of proteoglycans and glycoproteins is involved in granule formation in pancreatic acinar cells. * Author for correspondence (e-mail: [email protected] ) 25 05 2000 27 03 2000 © 2000 by Company of Biologists 2000 Rat Pancreas Pancreatic zymogen...

... previously shown that, in the presence of SCF, there were more type A spermatogonia incorporating thymidine at stage XII of rat seminiferous tubules cultured in vitro than in the absence of SCF, implying that the increased DNA synthesis might result from enhanced survival of spermatogonia. To explore...

...Antonio Giordano; Manrico Morroni; Flavia Carle; Rosaria Gesuita; Gian Franco Marchesi; Saverio Cinti ABSTRACT Rat periovarian adipose tissue contains a low number of uncoupling protein-expressing brown adipocytes scattered into lobules of white fat. Their increase following cold acclimation...

... examined the effects of all-trans retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the expression of α 1 (Cx43) and β 2 (Cx26) connexins, the two major gap junction gene products in mature rat epidermis. In fully differentiated, mature epidermis, α 1 is expressed in the lower, less...

...Mirella Meyer-Ficca; Jutta Müller-Navia; Harry Scherthan ABSTRACT Fluorescence in situ hybridization with centromeric, telomeric and whole chromosome paint probes was used to study nuclear topology in epididymal sperm as well as spermatids from testis tissue sections of the rat. Pericentromeric...

... 1998 © 1998 by Company of Biologists 1998 Osteoblast Osteopetrosis Actin Cytoskeleton Rat The actin cytoskeleton plays an essential role in cell structure and physiology. The cytoskeletal actin isoforms, β- and γ-, are ubiquitous components of the microfilament system. Among...

... of Biologists 1994 tropomyosin isoform antipeptide antiserum actin microfilament cytoskeleton cultured neuron cerebellum development rat Microfilaments are involved in neurite extension in the devel-oping neuron, where their main role is controlling growth cone motility ( Letourneau, 1983...

... this selectivity. Isolated rat hearts were perfusion-fixed through the coronary circulation, stained with lanthanum or tannic acid, and further processed for transmission electron microscopy. Thin sections viewed at X 160,000 magnification revealed regularly spaced, cleft-spanning structures in the wider zone...

...Michael Sendtner; Yoshihiro Arakawa; Kurt A. Stockli; Georg W. Kreutzberg; Hans Thoenen ABSTRACT We have demonstrated that the extensive degeneration of motoneurons in the rat facial nucleus after transection of the facial nerve in newborn rats can be prevented by local ciliary neurotrophic factor...

... immunogold-labeling procedure to determine the ultrastructural localization of RH in several different secretory cells of the rat. Label was present in nuclei, especially over the heterochromatin, and in the cytoplasm, particularly in areas containing rough endoplasmic reticulum. Immunolabeled RH was also...

...Paul Smith ABSTRACT Endothelial cells were obtained from the aortas of Wistar rats by collagenase digestion. Cells were grown to confluence in medium 199 enriched with L-glutamine but without specific growth factors. Cells were subcultured into 35 mm dishes or 25 cm 2 flasks coated with fibronectin...

...Bert Ph. M. Menco ABSTRACT Tight junctions of the olfactory epithelium of rat embryos were studied at the 14th day of gestation and during their subsequent development. Two different epithelial morphologies could be distinguished at the 14th gestational day. In one group of embryos the epithelial...