... homolog, Bph1. We show that Bph1 is an endosomal protein and an effector of the minor Rab5 isoform Ypt52. Strikingly, bph1 Δ mutant cells have lipidated Atg8 on their endosomes, which is sorted via late endosomes into the vacuole lumen under non-autophagy-inducing conditions. In agreement...

... the multivesicular body (MVB). Completion of MVB sorting is accompanied by maturation of the endosome into a late endosome, an event that requires inactivation of the early endosomal GTPase Rab5 (herein referring to generically to all isoforms). Here, we show that HD-PTP links ESCRT function with endosomal...

...Frode Miltzow Skjeldal; Linda Hofstad Haugen; Duarte Mateus; Dominik M. Frei; Anna Vik Rødseth; Xian Hu; Oddmund Bakke ABSTRACT Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late...

... activation, its trafficking through the endosomal system and its subsequent downregulation. EGFR Endosomes ER Signaling Rab7 Rab5 European Research Council 10.13039/100010663 Multicellular life necessitates communication between distantly located cells in a manner...

.... Published by The Company of Biologists Ltd 2015 Summary: Retrograde transport is not required for cytosolic translocation of the B-subunit of Shiga toxin, revealing new facets of the endosomal escape process with relevance to antigen cross-presentation. Shiga toxin Rab6 Rab5 Rab7 Sec22B...

... purification and mass spectrometry, we identify a complex containing RasGAP together with Sprint, which is a Ras effector and putative activator of the endocytic GTPase Rab5. Formation of the RasGAP–Sprint complex requires the SH2 domains of RasGAP and tyrosine phosphorylation of Sprint. RasGAP and Sprint...

...Jorge Díaz; Pablo Mendoza; Rina Ortiz; Natalia Díaz; Lisette Leyton; Dwayne Stupack; Andrew F. G. Quest; Vicente A. Torres ABSTRACT Rab5 is a small GTPase that regulates early endosome trafficking and other cellular processes, including cell adhesion and migration. Specifically, Rab5 promotes Rac1...

..., the small GTPase Rab5, a crucial regulator of early endosome dynamics, promotes cell migration in vitro and in vivo . Precisely how Rab5 participates in these events remains to be determined. Considering that focal adhesions represent structures crucial to cell migration, we specifically asked whether Rab5...

... protein receptors (SNAREs) and can tether membranes. Owing to the presence of specific subunits, CORVET is a Rab5 effector complex, whereas HOPS can bind efficiently to late endosomes and lysosomes through Rab7. Based on the recently described overall structure of the HOPS complex and a number of in vivo...

... is determined in part by the RAS effector RIN1, a guanine nucleotide exchange factor (GEF) for RAB5 GTPases. EGFR degradation was slowed by RIN1 silencing, enhanced by RIN1 overexpression and accelerated by RIN1 localization to the plasma membrane. RIN1 also directly activates ABL tyrosine kinases, which...

...- and Rab5-dependent endocytosis leads to a phenotype that is undistinguishable from that of the ninaE -null mutant. Importantly, the corresponding endocytic activity is essential to prevent early onset of rhabdomere degeneration. In conclusion, we propose that Rh1 function in promoting RTW morphogenesis...

... and larvae. We found that the IAC undergoes turnover in MTJs and that this process is mediated by clathrin-dependent endocytosis. Moreover, the small GTPase Rab5 can regulate the proportion of IAC components that undergo turnover. Also, altering Rab5 activity weakened MTJs, resulting in muscle defects...

... LRP6 from the lipid raft. Dkk1 internalized LRP6 in a Rab5-dependent mechanism to prevent phosphorylation mediated by CK1γ. The internalized LRP6 was recycled back in a Rab11-dependent mechanism to the cell-surface membrane, and the recycled LRP6 again responded to Wnt3a and Dkk1. Internalized Dkk1...

...Julien Compagnon; Louis Gervais; Mabel San Roman; Sophy Chamot-Bœuf; Antoine Guichet Phosphoinositides have emerged as key regulators of membrane traffic through their control of the localization and activity of several effector proteins. Both Rab5 and phosphatidylinositol (4,5)-bisphosphate...

... suggest a role in endocytosis, and recently it has been shown that huntingtin interacts indirectly with the early endosomal protein Rab5 through HAP40. Here we show that Rab5 inhibition enhanced polyglutamine toxicity, whereas Rab5 overexpression attenuated toxicity in our cell and fly models of HD. We...

...Xinjun Zhang; Xi He; Xin-Yuan Fu; Zhijie Chang The small GTPases Rab5 and Rab21 are closely related, and play essential roles in endocytic trafficking. Rab5 is regulated by VPS9-domain-containing guanine nucleotide exchange factors. Here, we describe a new VPS9-domain protein with ankyrin repeats...

...Emilia Galperin; Alexander Sorkin Rab5 is a small GTPase that controls endocytosis and early endosome dynamics. To visualize active, GTP-loaded Rab5 in living cells, we developed molecular sensors consisting of the Rab5-binding fragments of Rabaptin5 or EEA.1 fused to yellow fluorescent protein...

...Hideyuki Shimizu; Satoru Kawamura; Koichi Ozaki Rab5 small GTPase is a famous regulator of endocytic vesicular transport from plasma membrane to early endosomes. In neurons, Rab5 is found not only on endocytic vesicles in cell bodies but also on synaptic vesicles in nerve terminals. However...

.... LPA 1 internalization was strongly inhibited by dominant-inhibitory mutants of both dynamin2 (K44A) and Rab5a (S34N). In addition, both dynamin2 K44A and Rab5 S34N mildly inhibited LPA 1 -dependent activation of serum response factor. Finally, our results also indicate that LPA 1 exhibits basal, LPA...

...Yang Hu; Jen-Zen Chuang; Kai Xu; Timothy G. McGraw; Ching-Hwa Sung Rab5, a member of the small GTPase family of proteins, is primarily localized on early endosomes and has been proposed to participate in the regulation of early endosome trafficking. It has been reported that phosphatidylinositol 3...