...- methylation (2′ -O- Me) of ribosomal RNA (rRNA) shows plasticity that is potentially associated with cell phenotypes. We used RiboMeth-seq profiling to reveal growth arrest-specific 2′ -O- Me patterns in primary human dermal fibroblasts from three different donors. We exposed cells to hydrogen peroxide...

...Syed A. Ali; Jason R. Dobson; Jane B. Lian; Janet L. Stein; Andre J. van Wijnen; Sayyed K. Zaidi; Gary S. Stein The osteogenic and oncogenic transcription factor RUNX2 downregulates the RNA polymerase I (RNA Pol I)-mediated transcription of rRNAs and changes histone modifications associated...

... in nucleoli, suggesting that nucleoli are the site of USP36 action. USP36 deubiquitylated the nucleolar proteins nucleophosmin/B23 and fibrillarin, and stabilized them by counteracting ubiquitylation-mediated proteasomal degradation. RNAi-mediated depletion of cellular USP36 resulted in reduced levels of rRNA...

... Methylation Transcriptional repression rRNA Eukaryotic interphase nuclei are highly compartmentalised structures. It is generally accepted that architectural organisation of the nucleus and regulation of transcription are functionally linked. For example, it has been proposed that RNAs...

.... These results suggest that the dense fibrillar component is the site of rRNA transcription. After dispersing the granular component and the dense fibrillar component by a hypotonic treatment, removal of most chromatin and preparation of resinless sections, fibrillar centres remained fixed to a nucleoskeleton...

..., argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and compo- sition may...

... rRNA © 1991 by Company of Biologists 1991 08 03 1991 16 05 1991 * Author for correspondence and reprints Fig. 19. Material as shown in Fig. 18 . A small nuclear inclusion fails to bind the ribosomal probe. Electron micrograph. Bar, 0.5 μm. Fig. 20...

...Marc Thiry; Guy Goessens ABSTRACT The precise location of transcribing rRNA genes within Ehrlich tumor cell nucleoli has been investigated using two approaches: high-resolution autoradiography of cells pulse-labelled with tritiated uridine, varying the exposure time, and in situ-in vitro...

... Bbckingham , K and Rubacha , A. ( 1984 ) Different chromatin states of the intron- and type 1 intron + rRNA genes of Calliphora erythrocephala . Chromosoma 90 , 311 – 316 . 10.1007/BF00287040 Bedo , D. G and Webb , G. C. ( 1989 ). Conservation of nucleolar structure...