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Keywords: protoplasts
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Journal Articles
Journal Articles
J Cell Sci (1993) 106 (2): 555–565.
Published: 1 October 1993
..., protoplasts, a cell wallless mutant, and experimentally generated small, flattened cell fragments of the mutant cells. Organelle movements were visualized by DIC optics and computer-enhanced video microscopy. In all cell models the transport of organelles was vectorial and saltatory in nature. The mean...
Journal Articles
J Cell Sci (1993) 105 (1): 263–268.
Published: 1 May 1993
... to obtain pref-erentially the extrusion of protoplasm, or to gain access to a small area of plasma membrane in situ. The com-plete protoplasm can be expelled, to form a protoplast. Fluorescein diacetate staining indicated esterase activity and membrane integrity of the protoplasts. Microscopic examination...
Journal Articles
J Cell Sci (1991) 98 (2): 169–174.
Published: 1 February 1991
...Tomohiro Akashi; Hiroh Shibaoka ABSTRACT Abundant microtubules (MTs) were present both in protoplasts isolated from tobacco BY-2 cells and on membrane ghosts prepared from such protoplasts. However, only a few MTs or none at all were observed on membrane ghosts prepared from protoplasts pretreated...