.... Heterozygous ttn5-1 plants can display reduced root iron reductase activity. This activity is needed for iron uptake via IRT1. Fluorescent fusion proteins of TTN5 and IRT1 colocalize at locations where IRT1 sorting and cycling between the plasma membrane and the vacuole are coordinated. TTN5 can also interact...

... that TTN5 can be present at the plasma membrane and that it dynamically associates with membranes of vesicles, Golgi stacks and multivesicular bodies. Although TTN5 Q70L mirrored wild-type TTN5 behavior, the TTN5 T30N mutant differed in some aspects. Hence, the unusual rapid nucleotide exchange activity...

...Stéphane Vassilopoulos; Guillaume Montagnac ABSTRACT Clathrin assembles into honeycomb-like lattices at the plasma membrane but also on internal membranes, such as at the Golgi and tubular endosomes. Clathrin assemblies primarily regulate the intracellular trafficking of different cargoes...

...Rachel C. Wills; Colleen P. Doyle; James P. Zewe; Jonathan Pacheco; Scott D. Hansen; Gerald R. V. Hammond ABSTRACT The lipid molecule phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P 2 ] controls all aspects of plasma membrane (PM) function in animal cells, from its selective permeability...

...Katherine M. Paine; Kamilla M. E. Laidlaw; Gareth J. O. Evans; Chris MacDonald ABSTRACT The yeast (Saccharomyces cerevisiae) plasma membrane (PM) is organised into specific subdomains that regulate surface membrane proteins. Surface transporters actively uptake nutrients in particular regions...

...-dependent mechanism to promote successful cytokinesis in adherent mammalian cells. Cytokinesis Mitosis Plasma membrane Protocadherin PCDH7 Palmitoylation ZDHHC5 Cleavage furrow Actomyosin Plasticity BioID Türkiye Bilimsel ve Teknolojik Araştırma Kurumu 116Z305 Cell...

...Lingxiao Tan; Kwang-Jin Cho; Walaa E. Kattan; Christian M. Garrido; Yong Zhou; Pratik Neupane; Robert J. Capon; John F. Hancock ABSTRACT The primary site for KRAS signaling is the inner leaflet of the plasma membrane (PM). We previously reported that oxanthroquinone G01 (G01) inhibited KRAS PM...

... plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation. The construct continued to mark the PM at the root hair apical dome, but only during periods of reduced growth. During rapid tip growth, AGD1-GFP labeled the PM of the lateral flanks...

... that are apposed to the plasma membrane. They form a rim of filaments interconnecting the desmosomes in a circumferential network. We hypothesize that they are part of a rim-and-spoke arrangement of IFs in epithelia. From our review of the literature, we extend this functional role for the subplasmalemmal rim...

..., into the blood stream. Before release, the final step of merozoite development is the assembly of the cortical pellicle, a multi-layered membrane structure. This unique apicomplexan feature includes the inner membrane complex (IMC) and the parasite's plasma membrane. A dynamic ring structure, referred...

...Tomáš Chum; Daniela Glatzová; Zuzana Kvíčalová; Jan Malínský; Tomáš Brdička; Marek Cebecauer ABSTRACT Plasma membrane proteins synthesised at the endoplasmic reticulum are delivered to the cell surface via sorting pathways. Hydrophobic mismatch theory based on the length of the transmembrane domain...

...Derek C. Prosser; Anthony E. Pannunzio; Jeffrey L. Brodsky; Jeremy Thorner; Beverly Wendland; Allyson F. O'Donnell ABSTRACT Clathrin-mediated endocytosis (CME) is a well-studied mechanism to internalize plasma membrane proteins; however, to endocytose such cargo, most eukaryotic cells also use...

...Ruth Kabeche; Louisa Howard; James B. Moseley ABSTRACT Cell surface area rapidly increases during mechanical and hypoosmotic stresses. Such expansion of the plasma membrane requires ‘membrane reservoirs' that provide surface area and buffer membrane tension, but the sources of this membrane remain...

... released from thrombin‐activated platelets lost membrane asymmetry. Our results suggest that the microparticles were shed from platelet plasma membrane domains enriched with phosphatidylserine and/or phosphatidylinositol at the outer leaflet. These findings underscore the strict regulation and cell‐type...

...Masashi Maekawa; Gregory D. Fairn ABSTRACT Cholesterol is an essential component of metazoan cellular membranes and it helps to maintain the structural integrity and fluidity of the plasma membrane. Here, we developed a cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium...

...Saori Yamauchi; Keisuke Obara; Kenya Uchibori; Akiko Kamimura; Kaoru Azumi; Akio Kihara ABSTRACT Plasma membrane lipid asymmetry is important for various membrane-associated functions and is regulated by membrane proteins termed flippases and floppases. The Rim101 pathway senses altered lipid...

...Maria F. Garcia-Parajo; Alessandra Cambi; Juan A. Torreno-Pina; Nancy Thompson; Ken Jacobson ABSTRACT Early studies have revealed that some mammalian plasma membrane proteins exist in small nanoclusters. The advent of super-resolution microscopy has corroborated and extended this picture, and led...

.... These effectors function in vesicle formation and tethering, non-vesicular lipid transport and cytoskeletal regulation. Beyond fundamental membrane trafficking roles, Arf proteins also regulate mitosis, plasma membrane signaling, cilary trafficking and lipid droplet function. Tight spatial and temporal regulation...

... in leukocytes and sensory neuron contributes to the pathophysiology of autoimmune diseases and sensory syndromes. Molecular mechanisms underlying Kv1.3 channel trafficking to the plasma membrane remain elusive. We report a novel non-canonical di-acidic signal (E483/484) at the C-terminus of Kv1.3 essential...

... of an intracellular FLT3-ITD/Ras signaling pathway by comparing Ras signaling of FLT3 ITD with that of wild-type FLT3. Ligand stimulation activated both K- and N-Ras in cells expressing wild-type FLT3. Live-cell Ras–GTP imaging revealed ligand-induced Ras activation at the plasma membrane (PM). FLT3-ITD-dependent...