... forms of Drp1. Importantly, downregulation of GTPBP8 caused an increase in oxidative stress, modulating cell signaling involved in the increased phosphorylation of Drp1 at Ser637. This phosphorylation hindered the recruitment of Drp1 to mitochondria, leading to mitochondrial fission defects. By contrast...

... Rho GTPase RhoU self-associates via its C-terminal extension. This interaction increases activation of downstream p21-activated kinases and regulates cell morphology, constituting a new mechanism of Rho GTPase regulation. RhoU Rho GTPases Cell morphology Phosphorylation p21-activated kinase...

..., lipidation and phosphorylation, as well as their known contributions to the autophagy process, physiology and disease. References Abreu , S. , Kriegenburg , F. , Gómez-Sánchez , R. , Mari , M. , Sánchez-Wandelmer , J. , Rasmussen , M. S. , Guimarães , R. S. , Zens , B...

..., a small pool is retained in eisosomes to provide efficient recovery from starvation. We find the core eisosome subunit Pil1, a Bin, Amphiphysin and Rvs (BAR) domain protein required for eisosome biogenesis, is phosphorylated primarily by the kinase Pkh2. In response to acute glucose starvation, Pil1...

...Tadayuki Komori; Tomoki Kuwahara; Tetta Fujimoto; Maria Sakurai; Ikuko Koyama-Honda; Mitsunori Fukuda; Takeshi Iwatsubo ABSTRACT Rab proteins are small GTPases that regulate a myriad of intracellular membrane trafficking events. Rab29 is one of the Rab proteins phosphorylated by leucine-rich repeat...

...Sophie Grziwa; Jan-Hannes Schäfer; Raffaele Nicastro; Annabel Arens; Claudio De Virgilio; Florian Fröhlich; Arne Moeller; Jieqiong Gao; Lars Langemeyer; Christian Ungermann ABSTRACT The Saccharomyces cerevisiae casein kinase protein Yck3 is a central regulator at the vacuole that phosphorylates...

...Jaime Santo-Domingo; Steve Lassueur; Antonio Núñez Galindo; Pilar Alvarez-Illera; Silvia Romero-Sanz; Elena Caldero-Escudero; Sergio de la Fuente; Loïc Dayon; Andreas Wiederkehr ABSTRACT Glucose sensing in pancreatic β-cells depends on oxidative phosphorylation and mitochondria-derived signals...

... by the LMNA gene, contributes towards altered gene expression during HS, but the underlying mechanisms are poorly understood. Here, we show that upon HS, lamin A/C was reversibly phosphorylated at serine 22 in concert with HSF1 activation in human cells, mouse cells and Drosophila melanogaster in vivo...

... for the selection and ER export of transmembrane proteins. In this work, we show the importance of a phosphorylation consensus site (S134) at the C-terminus of Erv14. Mimicking phosphorylation of S134 (S134D) prevents the incorporation of Erv14 into COPII vesicles, delays cell growth, exacerbates growth of sec...

...) are positioned. Using intestinal organoids, we show that the shuttling of the Mcc protein depends on phosphorylation by casein kinases 1δ and ε, which are critical modulators of WNT signaling. Together, our findings support a role for MCC in establishing and maintaining the cellular architecture...

... phosphorylation by mass spectrometry and in vitro kinase assay. Focusing on S131 and S146, we demonstrate that they have a role in SHARPIN-ARP2/3 complex interaction, but play no role in integrin inhibition or LUBAC activation. Consistent with its novel role in ARP2/3 regulation, S146 phosphorylation of SHARPIN...

... with cancer development. We have recently shown that PRICKLE1, a core Wnt/PCP pathway component, is a marker of poor prognosis in triple-negative breast cancer (TNBC). PRICKLE1 is phosphorylated by the serine/threonine kinase MINK1 and contributes to TNBC cell motility and invasiveness. However, the identity...

.... 4 5 2021 9 11 2021 © 2021. Published by The Company of Biologists Ltd 2021 Summary: Frizzled3 induces Vangl2 phosphorylation and promotes PCP in Xenopus neuroectoderm by inhibiting the formation of the anterior Vangl2–Prickle3 complex. Vangl2 Phosphorylation PCP...

... phosphorylation. However, the in vivo relevance of the phosphorylation of WAVE proteins remains largely unknown. Here, we identified casein kinase I alpha (CK1α) as a regulator of WAVE, thereby controlling cell shape and cell motility in Drosophila macrophages. CK1α binds and phosphorylates WAVE in vitro...

... brassinosteroid and phytosulfokine signalling, which orchestrate diverse cellular responses. However, it is unclear how these activities are coordinated. Here, we show that RLP44 is phosphorylated in its highly conserved cytosolic tail and that this post-translational modification governs its subcellular...

... long-term potentiation and synaptic upscaling, AMPARs are accumulated at synapses to increase synaptic strength. Neuronal activity leads to phosphorylation of AMPAR subunit GluA1 (also known as GRIA1) and subsequent elevation of GluA1 surface expression, either by an increase in receptor forward...

... yeast Schizosaccharomyces pombe . Because cell cycle-regulated phosphorylation of the central intrinsically disordered region (IDR) of the Imp2 paralog Cdc15 controls Cdc15 oligomerization state, localization and ability to bind protein partners, we investigated whether Imp2 is similarly...

... ). Kindlin-2 phosphorylation by Src at Y193 enhances Src activity and is involved in Migfilin recruitment to the focal adhesions . FEBS Lett. 589 , 2001 - 2010 . 10.1016/j.febslet.2015.05.038 Luo , B.-H. , Carman , C. V. and Springer , T. A. ( 2007 ). Structural basis...

... that the spindle assembly factor TPX2 regulates astral microtubules. TPX2 in the spindle pole area is activated by GM130 (GOLGA2) on Golgi membranes to promote astral microtubule growth. GM130 relieves TPX2 inhibition by competing for importin α1 (KPNA2) binding. Mitotic phosphorylation of importin α at serine 62...

... the WNT–Frizzled–Dishevelled axis, and (2) is the AGS3 and DVL2 interaction regulated? The interaction of AGS3 and DVL2 was regulated by protein phosphorylation, subcellular distribution, and a cell-surface G-protein-coupled receptor. These data, and the commonality of functional system impacts observed...