... with actin organizes tight junctions to control epithelial permeability . Dev. Cell 54 , 792 - 804.e7 . 10.1016/j.devcel.2020.07.022 Bement , W. M. , Goryachev , A. B. , Miller , A. L. and von Dassow , G. ( 2024 ). Patterning of the cell cortex by Rho GTPases . Nat. Rev...

... present current knowledge on the molecular components and pathways that establish this selectively permeable barrier and the interactions that lead to barrier dysfunction during disease. Although this discussion has focused on inflammatory bowel disease and graft-versus-host disease, which has been...

...-dependent phosphorylation have been implicated in the loosening of cell–cell contacts, thereby modulating vascular permeability. Here we demonstrate that BMP6 induces hyperpermeabilization of human endothelial cells by inducing internalization and c-Src-dependent phosphorylation of VE-cadherin. Furthermore...

... are well defined, whereas the purpose of RhoC remains poorly understood. Here, we describe how RhoC promotes vascular homeostasis by modulating endothelial cell migration, proliferation and permeability. RhoC stimulates proliferation of human umbilical vein endothelial cells (HUVECs) by stabilizing nuclear...

..., ischemia and diabetes – which leads to severe, and sometimes fatal, organ dysfunction. Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherens junctions (AJs). In endothelial cells, AJs are largely composed of vascular endothelial cadherin (VE-cadherin...

... endothelial cells. Because cortactin is known to regulate cellular permeability through its interaction with the actin cytoskeleton, a process that is crucial for endothelial cell homeostasis, the role of Tbdn on endothelial cell permeability was examined. Knockdown of Tbdn expression in endothelial cells led...

.... * Author for correspondence (e-mail: [email protected] ) 24 9 2007 © The Company of Biologists Limited 2008 2008 Adenylyl cyclase Lung Permeability Lung endothelium forms a thin but tightly regulated barrier that limits the movement of fluids and molecules from...

... application we demonstrated that the strength of adhesion of VE-cadherin-coated microspheres to the surface of cultured endothelial monolayers is significantly reduced by treatment with two well-established permeability-increasing compounds,cytochalasin D and the Ca 2+ -ionophore A23187, which shows that both...

... membrane. * Author for correspondence (e-mail: [email protected] ) 23 4 2002 © The Company of Biologists Limited 2002 2002 SPARC Lens capsule Basement membrane Extracellular matrix Epithelial cells Fiber cells Collagen IV Cataract Permeability Targeted...

...Marco Wachtel; Karl Frei; Elisabeth Ehler; Adriano Fontana; Kaspar Winterhalter; Sergio M. Gloor ABSTRACT Regulation of epithelial and endothelial permeability is essential for proper function of compartmentalized organisms, and tyrosine phosphorylation plays an important role in this process. We...

... of junctional VE-cadherin staining and loss of cell-cell adhesion. In functional assays, inhibition of VE-cadherin caused increased monolayer permeability and enhanced neutrophil transendothelial migration. In a complementary set of experiments, modulation of the actin cytoskeleton was found to strongly affect...

... integrity. Cell monolayers failed to develop an efficient permeability barrier, as demonstrated by low transcellular electrical resistance values and an increased paracellular flux to small molecular mass tracers. Furthermore, gaps were found to have been induced in the P-face associated tight junction...

... of treatment and attaining a fourfold higher level of permeance by 24 hours. This elevated level was maintained with continuous exposure to 8-bromo-cAMP for seven days. The permeability change was accompanied by an increase in gap junctions as shown by freeze-fracture electron microscopy and by confocal...

... freeze-fracture electron microscopy, and ion and inulin permeability. The complexity of tight junctions, defined as the number of branch points per unit length of tight junc- tional strands, decreased 5 hours after culture but there- after remained almost constant. In contrast, the association of tight...

... and Engler, 1990 ; Thornton et al., 1989 ), inflammatory disorders ( Fiebig et al., 1991 ; Grant, 1973 ; Jutila, 1992 ) and vascular permeability disorders ( Kubes et al., 1991a ; Schoenberg et al., 1991 ). Their action is mainly due to a rolling phenomenon along venular walls, adhesion to vascular...

...Taichi Takeda; Yuko Yamashita; Syuji Shimazaki; Youji Mitsui ABSTRACT To determine if histamine acts directly on the vascular endothelium, the effect of histamine on the permeability of cultured human endothelial cell monolayers and the role of second messengers were examined. The addition of 10 −6...