... gametophyte development. Combining an inducible knockdown approach and in vivo pH measurements, we show here that reduced ClC activity does not affect pH in the TGN/EE but causes hyperacidification of trans -Golgi cisternae. Taken together, our results show that ClC-mediated anion transport into the TGN/EE...

...Katharine A. White; Bree K. Grillo-Hill; Diane L. Barber ABSTRACT Dysregulated pH is a common characteristic of cancer cells, as they have an increased intracellular pH (pH i) and a decreased extracellular pH (pH e) compared with normal cells. Recent work has expanded our knowledge of how...

...Dhiman Sankar Pal; Mazharul Abbasi; Dipon Kumar Mondal; Binitha Anu Varghese; Ritama Paul; Shalini Singh; Rupak Datta ABSTRACT Leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. How Leishmania cells maintain near-neutral intracellular pH...

... for genesis of the digestive vacuole and transfer of haemoglobin from the host cytoplasm are still debated. Here, we use live-cell imaging and photobleaching to monitor the uptake of the pH-sensitive fluorescent tracer SNARF-1-dextran from the erythrocyte cytoplasm in ring-stage and trophozoite-stage...

... peroxisomal function, but the nature of the peroxisomal pH has remained inconclusive and little is known about its generation. To determine the pH of Sacharomyces cerevisiae peroxisomes in vivo, we have used two different pH-sensitive yellow fluorescent proteins targeted to the peroxisome by virtue of a C...

... stage, and have an abnormal ratio of prestalk and prespore cells. RtoA is also involved in fusion of endocytic/exocytic vesicles. Cells lacking RtoA, although having a normal endocytosis rate, have a decreased exocytosis rate and endosomes with abnormally low pHs. RtoA levels vary during the cell cycle...

...Stephen M. Richards; Marisa E. Jaconi; Guy Vassort; Michel Pucéat ABSTRACT The anion exchangers (AE) are encoded by a multigenic family that comprises at least three genes, AE1, AE2 and AE3, and numerous splicoforms. Besides regulating intracellular pH (pH i) via the Cl − /HCO 3 − exchange, the AEs...

...Mary K. L. Collins; Isla J. Furlong; Prupti Malde; Rosalia Ascaso; Javier Oliver; Abelardo Lopez Rivas ABSTRACT DNA fragmentation in isolated nuclei from the murine IL3-dependent bone marrow cell line BAF3 could be stimulated either by decreasing pH below 6.5 or by adding μM calcium at neutral pH...

... reversed 10 minutes after cells were returned to 37°C. We also used flow cytofluori-metric measurements of pH dependent fluorescence quenching to measure the pH of the terminal endocytic compartment. Fluoresceinated lectins accumulated in a terminal compartment with a pH of 6.0-6.1, a value con-siderably...

...-cytoskeletons, on the assumption that inhibition of the release of myosin II keeps the myosin II in the cortical region, and is responsible for the localization of myosin II in the cortical region. The release of myosin II is inhibited at pH values below 6.5. This effect is not due to the inhibition of heavy...

... the nuclear cycle, and (2) by raising the cytoplasmic pH from that of unfertilized cytoplasm to that of fertilized, which activated the nuclear cycle and initiated tubulin polymerization. In the unactivated eggs, with increasing concentrations of the inducing agents, temperature and duration of treatment...

...D. G. Cran; R. M. Moor; R. F. Irvine ABSTRACT Microinjection of inositol 1,4,5–trisphosphate into sheep and hamster oocytes induces secretion of cortical granules in a dose-dependent manner. In the sheep, this effect is strongly pH-dependent with minimal exocytosis taking place at pH 6·8 but a full...

... of the unpigmented surface induced by these treatments is due to conformational changes of surface material. 20 09 1985 19 10 1985 © 1986 by Company of Biologists 1986 surface contraction amphibian eggs ionic strength pH trypsin. During studies on the contractility of amphibian...