... of cytosolic and nuclear survivin with p34cdc2. Asynchronous (A, Async.) or taxol-arrested (B, Txl) HeLa cells were immunoprecipitated with pAb NOVUS or mAb 32.1. Aliquots of pellet(P) or supernatant (S) were sequentially immunoblotted with pAb NOVUS or a mAb to p34cdc2 (arrows). (C) Differential...

... cells ( Lee and Nurse, 1987 ; Gautier et al . 1988) and a homologous protein that is involved in the cell cycle of the unicellular plant Chlamydomonas ( John et al . 1989 ). After affinity purification by binding to mammalian p34cdc2 the antibody recognised a wheat protein also of M r 34×10...

... or diacylglycerol, the activity of which becomes maximal when cells enter M phase, decreases at ana-telophase, and is low during interphase, has been purified to near homogeneity from starfish oocytes and its catalytic subunit identified as p34cdc2. M phase-promoting factor (MPF) was found to co-purify with the M...