...Sarah Klapproth; Karsten Richter; Clara Türk; Theresa Bock; Thomas Bromberger; Julian Dominik; Kathrin Huck; Kristian Pfaller; Michael W. Hess; Christoph A. Reichel; Marcus Krüger; Inaam A. Nakchbandi; Markus Moser ABSTRACT Osteoclasts form special integrin-mediated adhesion structures called...

...Maayan Barnea-Zohar; Sabina E. Winograd-Katz; Moran Shalev; Esther Arman; Nina Reuven; Lee Roth; Ofra Golani; Merle Stein; Fadi Thalji; Moien Kanaan; Jan Tuckermann; Benjamin Geiger; Ari Elson ABSTRACT Homozygosity for the R51Q mutation in sorting nexin 10 (SNX10) inactivates osteoclasts (OCLs...

...Anne Blangy; Guillaume Bompard; David Guerit; Pauline Marie; Justine Maurin; Anne Morel; Virginie Vives ABSTRACT Osteoclasts are giant multinucleated myeloid cells specialized for bone resorption, which is essential for the preservation of bone health throughout life. The activity of osteoclasts...

...David Guérit; Pauline Marie; Anne Morel; Justine Maurin; Christel Verollet; Brigitte Raynaud-Messina; Serge Urbach; Anne Blangy ABSTRACT Among hematopoietic cells, osteoclasts (OCs) and immature dendritic cells (DCs) are closely related myeloid cells with distinct functions: OCs participate...

...Dinisha Cyril Pirapaharan; Jacob Bastholm Olesen; Thomas Levin Andersen; Sandra Bjerre Christensen; Per Kjærsgaard-Andersen; Jean-Marie Delaisse; Kent Søe ABSTRACT Osteoblast lineage cells in human bone were recently shown to colonize eroded bone surfaces and to closely interact with osteoclasts...

...Kent Søe; Jean-Marie Delaissé ABSTRACT Bone erosion both demands that the osteoclast resorbs bone matrix and moves over the bone surface. It is widely accepted that these two activities alternate, because they are considered mutually exclusive since resorption is believed to involve an immobilizing...

...Heiani Touaitahuata; Anne Morel; Serge Urbach; Julio Mateos-Langerak; Sylvain de Rossi; Anne Blangy ABSTRACT Bone resorption by osteoclasts is mediated by a typical adhesion structure called the sealing zone or actin ring, whose architecture is based on a belt of podosomes. The molecular mechanisms...

.... Here, we investigated its functional relevance during osteoclast differentiation. KCNK1 was significantly induced during osteoclast differentiation, but its functional overexpression significantly inhibited osteoclast differentiation induced by RANKL (also known as TNFSF11), which was accompanied...

...Tristan W. Fowler; Archana Kamalakar; Nisreen S. Akel; Richard C. Kurten; Larry J. Suva; Dana Gaddy ABSTRACT The process of osteoclastic bone resorption is complex and regulated at multiple levels. The role of osteoclast (OCL) fusion and motility in bone resorption are unclear, with the movement...

... the temporal molecular steps of the de novo assembly and disassembly of podosomes in cultured osteoclasts. We demonstrate here that the earliest visible step in podosome assembly is the local accumulation of the plaque protein paxillin, along with cortactin, which stabilizes actin networks, followed by robust...

...Jiro Takito; Masanori Nakamura; Masaki Yoda; Takahide Tohmonda; Shinichi Uchikawa; Keisuke Horiuchi; Yoshiaki Toyama; Kazuhiro Chiba Multinucleated osteoclasts are responsible for bone resorption. Hypermultinucleated osteoclasts are often observed in some bone-related diseases such as Paget's...

...Monica Croke; F. Patrick Ross; Matti Korhonen; David A. Williams; Wei Zou; Steven L. Teitelbaum Cdc42 mediates bone resorption principally by stimulating osteoclastogenesis. Whether its sister GTPase, Rac, meaningfully impacts upon the osteoclast and, if so, by what means, is unclear. We find...

...Dafna Geblinger; Lia Addadi; Benjamin Geiger Bone resorption by osteoclasts depends on the assembly of a specialized, actin-rich adhesive ‘sealing zone’ that delimits the area designed for degradation. In this study, we show that the level of roughness of the underlying adhesive surface has...

...Eun-Ju Chang; Jeongim Ha; Hao Huang; Hyung Joon Kim; Jung Hoon Woo; Youngkyun Lee; Zang Hee Lee; Ju Han Kim; Hong-Hee Kim Osteoclastogenesis involves the commitment of macrophage-lineage precursors to tartrate-resistant acid phosphatase-positive (TRAP + ) mononuclear pre-osteoclasts (pOCs...

... postnatal bone homeostasis. ‡ Author for correspondence (e-mail: [email protected] ) * These authors contributed equally to this work 25 4 2007 © The Company of Biologists Limited 2007 2007 Smad4 Osteoblast Osteoclast Bone mass RANKL/OPG Bone remodeling...

...Eun-Ju Chang; Hyon Jong Kim; Jeongim Ha; Hyung Joon Kim; Jiyoon Ryu; Kwang-Hyun Park; Uh-Hyun Kim; Zang Hee Lee; Hyun-Man Kim; David E. Fisher; Hong-Hee Kim The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating...

...Chen Luxenburg; J. Thomas Parsons; Lia Addadi; Benjamin Geiger Osteoclasts are large, multinucleated cells that adhere to bone via podosomes, and degrade it. During osteoclast polarization, podosomes undergo reorganization from a scattered distribution, through the formation of clusters and ring...

... on their anabolic effects acting on cells of the osteoblast lineage. In this study we provide evidence that Wnts also regulate osteoclast formation and bone resorption, through a mechanism involving transcriptional repression of the gene encoding the osteoclastogenic cytokine receptor activator of NFκB ligand...

...Beatrice B. Yaroslavskiy; Yongjun Zhang; Sara E. Kalla; Verónica García Palacios; Allison C. Sharrow; Yanan Li; Mone Zaidi; Chuanyue Wu; Harry C. Blair The osteoclast degrades bone in cycles; between cycles, the cell is motile. Resorption occurs by acid transport into an extracellular compartment...

...Martin Pfaff; Pierre Jurdic Macrophages and osteoclasts develop unique contact sites with the extracellular matrix called podosomes. Podosomes have been associated with migratory and invasive cell characteristics, but a basic mechanism outlining their function is lacking. We have used chicken...