... enables the visualisation of cell cycle-dependent distinct organellar organisation in the human pathogen Candida albicans , and is generalised to the study of other pathogenic yeasts. Ultrastructure expansion microscopy Candida albicans Spindle pole bodies Nucleolus Microtubules Fungal...

...Koichiro Maki; Jumpei Fukute; Taiji Adachi In eukaryotic cell nuclei, specific sets of proteins gather in nuclear bodies and facilitate distinct genomic processes. The nucleolus, a nuclear body, functions as a factory for ribosome biogenesis by accumulating constitutive proteins, such as RNA...

... Chagas da Cunha ABSTRACT The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has antiproliferative and tumor-suppressive functions in some cellular contexts. In this work, we investigated how...

... is incompletely understood. Enhanced protein synthesis necessitates ribosome biogenesis at the nucleolus. Nucleolar size and activity are inextricably linked with altered gene expression. Here, we report changes in nucleolar morphology and function during myogenic differentiation. Immunofluorescence analysis...

... buds and micronuclei containing autophagy proteins in a basal way and in response to DNA double strand breaks, suggesting homeostatic nuclear maintenance by nucleophagy. Mammalian nucleophagy Autophagy DNA damage Nucleolus Micronuclei Consejo Nacional de Ciencia y Tecnología http...

...Milad Jeilani; Karen Billington; Jack Daniel Sunter; Samuel Dean; Richard John Wheeler ABSTRACT The compartmentalised eukaryotic cell demands accurate targeting of proteins to the organelles in which they function, whether membrane-bound (like the nucleus) or non-membrane-bound (like the nucleolus...

..., p110β and PtdIns(3,4,5) P 3 were localised in the nucleolus, which correlated with high levels of 47S pre-rRNA. p110β inhibition led to a decrease in both 47S rRNA levels and cell proliferation. In conclusion, these results present a nucleolar role for p110β that may contribute to tumorigenesis in EC...

.... The nucleus is organized into various subcompartments, with the nucleolus being the most prominent. We have conducted a candidate-based genetic screen for nuclear shape abnormalities in S. cerevisiae to ask whether the nucleolus influences nuclear shape. We find that increasing nucleolar volume triggers a non...

... nuclear functions. Chromatin Nuclear organization Nucleolus Phase separation National Institutes of Health 10.13039/100000002 U01 DA040601 Howard Hughes Medical Institute 10.13039/100000011 Compartmentalization is used throughout all kingdoms of biology...

... targeted nucleoli with carbon ions at the ion microbeam SNAKE. Localized ion irradiation with 1–100 carbon ions per point (about 0.3–30 Gy per nucleus) did not lead to overall reduced ribonucleotide incorporation in the targeted nucleolus or other nucleoli of the same cell. However, both 5-ethynyluridine...

.../ Summary: In humans, sirtuin 7 plays a key role in the synthesis of ribosomal subunits by controlling critical endonucleolytic cleavages of precursor ribosomal RNAs. Pre-rRNA processing Ribosome Sirtinol SIRT7 Nucleolus Deacetylase Centre National de la Recherche Scientifique 10.13039...

... and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two...

...Sugako Ogushi; Kazuo Yamagata; Chikashi Obuse; Keiko Furuta; Teruhiko Wakayama; Martin M. Matzuk; Mitinori Saitou ABSTRACT The mammalian oocyte nucleolus, the most prominent subcellular organelle in the oocyte, is vital in early development, yet its key functions and constituents remain unclear. We...

... B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes. * Author...

... nucleoli disappear, mitotic pre-ribosomal particles persist as observed in pre-nucleolar bodies (PNBs) during telophase. To further understand the relationship between the nucleolus and the PNBs, the presence and the fate of the mitotic pre-ribosomal particles during cell division were investigated. We...

...-like 3, GNL3, in invertebrate species). Several studies have recently pointed to an unexpected role of nucleostemin in safeguarding the genome integrity of stem and cancer cells. Since its discovery, the predominant presence of nucleostemin in the nucleolus has led to the notion that it might function...

... permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Aspirin Chemoprevention NF-κB Apoptosis Acetylation Nucleoli Nucleolus COMMD1 p300 XIAP The nuclear factor (NF)-κB transcription factor plays a central role...

... the nucleolus was fine-tuned for a role in genome protection and cell-cycle control as the vertebrates evolved. We thank Hanhui Ma in the T.P. laboratory for advice on the 5-EU assay. Author contributions T.L., L.M., T.P. and R.Y.L.T. designed research; T.L., L.M., T.-C.L. and L.J.W performed...

... by sequestering Cdc14 phosphatase in the nucleolus before mitotic exit. In this study, we show that dnt1 + positively regulates G2/M transition during the cell cycle. By conducting epistasis analyses to measure cell length at septation in double mutant (for dnt1 and genes involved in G2/M control) cells, we found...

... 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2...