...Takanobu Moriuchi; Fumiko Hirose ABSTRACT Dephosphorylation of lamin A, which triggers nuclear lamina reconstitution, is crucial for the completion of mitosis. However, the specific phosphatase and regulatory mechanism that allow timely lamin A dephosphorylation remain unclear. Here, we report...

...Shivnarayan Dhuppar; Aprotim Mazumder ABSTRACT Nuclear architecture is the organization of the genome within a cell nucleus with respect to different nuclear landmarks such as the nuclear lamina, nuclear matrix or nucleoli. Recently, nuclear architecture has emerged as a major regulator of gene...

... proteins’ that function to bridge heterochromatin and the nuclear membrane or nuclear lamina. We previously identified a human tethering protein, PRR14, that binds heterochromatin through an N-terminal domain, but the mechanism and regulation of nuclear lamina association remained to be investigated. Here...

... cytoskeleton, nuclear lamina and chromatin are critical for cellular responses and adaptation to external mechanical cues. In that respect, dynamic changes in the nuclear lamina and the surrounding cytoskeleton modify mechanical properties of the nucleus, thereby protecting genetic material from damage...

...; but how cells sense and transduce mechanical cues to modulate SRF−Mkl1-dependent gene expression is not well understood. Here, we demonstrate that the nuclear lamina-associated inner nuclear membrane protein Emerin stimulates SRF−Mkl1-dependent gene activity in a substrate stiffness-dependent manner...

...Jason M. Berk; Dan N. Simon; Clifton R. Jenkins-Houk; Jason W. Westerbeck; Line M. Grønning-Wang; Cathrine R. Carlson; Katherine L. Wilson ABSTRACT Emerin is a conserved membrane component of nuclear lamina structure. Here, we report an advance in understanding the molecular basis of emerin...

... with the nuclear localization signal to target assembly to the nuclear envelope . Cell 59 , 969 - 977 . Houben F. , Ramaekers F. C. , Snoeckx L. H. , Broers J. L. ( 2007 ). Role of nuclear lamina-cytoskeleton interactions in the maintenance of cellular strength . Biochim. Biophys...

...-catenin and emerin influenced each other's levels of expression and the onset of adipogenesis, suggesting that changes in the expression of nuclear lamina proteins were intimately linked to the stability of β-catenin. By contrast, dermal fibroblasts, which are emerin null, demonstrated increased nuclear...

... structures of the nuclear lamina were formed in senescent hMSCs, which are identified by the presence of Hayflick-senescence-associated factors. Notably, spatial changes in lamina shape were observed before the Hayflick senescence-associated factors, suggesting that the lamina morphology can be used...

...Martin W. Goldberg; Irm Huttenlauch; Christopher J. Hutchison; Reimer Stick Lamins are intermediate filament proteins and the major component of the nuclear lamina. Current views of the lamina are based on the remarkably regular arrangement of lamin LIII in amphibian oocyte nuclei. We have re...

..., B. ( 2006 ). Characterization of the Drosophila melanogaster genome at the nuclear lamina. Nat. Genet. 38 , 1005 -1014. Pirrotta, V. and Rastelli, L. ( 1994 ). White gene expression, repressive chromatin domains and homeotic gene regulation in Drosophila. BioEssays 16 , 549 -556...

...Kaliyaperumal Manju; Bhattiprolu Muralikrishna; Veena K Parnaik A-type lamins are components of the nuclear lamina. Mutations in the gene encoding lamin A are associated with a range of highly degenerative diseases termed laminopathies. To evaluate sensitivity to DNA damage, GFP-tagged lamin...

... and lamina morphology were abnormal in JIL-1 mutant egg chambers. The most common phenotypes observed were abnormal nurse cell nuclear lamina protrusions through the ring canals near the oocyte, as well as dispersed and mislocalized lamin throughout the egg chamber. These phenotypes were completely rescued...

... a network elastic modulus, unaffected by nucleoplasm, that averages 25 mN/m. Compared to plasma membranes of cells, the nuclear envelope is much stiffer and more resilient. Our results suggest that the nuclear lamina forms a compressed network shell of interconnected rods that is extensible but limited...

... binding from their indirect effects on emerin relocalization. * Author for correspondence (e-mail: [email protected] ) 3 4 2003 © The Company of Biologists Limited 2003 2003 Nuclear lamina Mouse knockout Nuclear envelope Emery-Dreifuss muscular dystrophy Lipodystrophy...

...Shobana S. Mani; Rithwick Rajagopal; Amanda B. Garfinkel; Xiaochun Fan; Mariana F. Wolfner The nuclear lamina provides an architectural framework for the nuclear envelope and an attachment site for interphase chromatin. In Drosophila eggs and early embryos its major constituent, lamin Dm 0...

.... *Author for correspondence (e-mail: [email protected] ) 15 10 2001 © The Company of Biologists Limited 2001 2001 Barrier to autointegration factor Emery-Dreifuss muscular dystrophy lamin A lamin-associated polypeptide 2 LEM-domain nuclear envelope nuclear lamina MAN1...

... termed the nuclear lamina. The lamina is considered to be an important determinant of interphase nuclear architecture as it plays an essential role in maintaining the integrity of the nuclear envelope and provides anchoring sites for chromatin ( Moir et al., 1995 ; Gant and Wilson, 1997 ; Stuurman et...

... TUNEL assay and completed before clustering of the nuclear pores. POM121 was eliminated significantly more rapid compared with NUP153 (a peripheral protein located in the nucleoplasmic basket of the nuclear pore complex) and lamin B (a component of the nuclear lamina). Disappearance of NUP153 and lamin...

... within the ER. Our data suggest that lamin A is essential for anchorage of emerin to the inner nuclear membrane and of lamin C to the lamina. 14 4 2001 © The Company of Biologists Limited 2001 2001 Lamins Emerin Nuclear envelope Nuclear lamina Emery-Dreifuss muscular dystrophy...