.... The ability to visualise endocytosis in vivo requires suitable assays to monitor the process. Here, we describe imaging-based assays to visualise endocytosis in the neuroepithelium of living zebrafish embryos. Injection of fluorescent tracers into the brain ventricles followed by live imaging was used...

...Cristina Afonso; Domingos Henrique Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all...

... into the extracellular space by exocytosis ( Février and Raposo, 2004 ; Stoorvogel et al., 2002 ; Théry et al., 2002 ), a process proposed to occur in the floorplate neuroepithelium ( Tanaka et al., 1988 ). The P2 particles, with an average diameter of 600 nm, are much larger than exosomes, which typically have...