...David L. Falk; Deborah Wessels; Leslie Jenkins; Tien Pham; Spencer Kuhl; Margaret A. Titus; David R. Soll Most cell types express two distinct forms of myosin I, amoeboid and short, distinguished by differences in their tail domains. Both types of myosin I have been implicated in the regulation...

...Christian Ruppert; Jasminca Godel; Rainer T. Müller; Ruth Kroschewski; Jutta Reinhard; Martin Bähler ABSTRACT Myr 1 is a widely distributed mammalian myosin I molecule related to brush border myosin I. A second widely distributed myosin I molecule similar to myr 1 and brush border myosin I, called...

... the terminal web. We propose that vesicles translocate from the Golgi to the apical cytoplasm along microtubules using dynein, and then move through the terminal web to reach the apical plasma membrane using the actin-based motor myosin-I. Our isolation of Golgi-derived vesicles possessing both myosin-I...

... kDa, and 110 kDa have been detected previously in the C2 BBe cells with a head-specific monoclonal antibody to avian brush border myosin I (M. D. Peterson and M. S. Mooseker (1992) J. Cell Sci . 102, 581-600). Each of these immunogens displayed distinct expression patterns during brush border assembly...

... the cell line Caco-2 (derived from a human colonic adenocarcinoma) formed a polarized monolayer with an apical BB morphologically comparable to that of the human colon. BBs could be isolated by standard methods and contained the microvillar proteins villin, fimbrin, sucrase-isomaltase and BB myosin I...

...Shigenobu Yonemura; Thomas D. Pollard ABSTRACT We used several fixation protocols and a panel of monoclonal antibodies to re-examine the localization of myosin I and myosin II in Acanthamoeba . Two monoclonal antibodies that bind to the head of myosin II stain a range of particles in the cytoplasm...

...Jennifer L. Hicks; David S. Williams ABSTRACT The Drosophila ninaC gene encodes for two head-specific proteins of 132 kDa and 174 kDa. Their predicted amino acid sequences indicate that they may have myosin I and kinase properties. We have: (1) determined the cellular and subcellular distributions...

...John A. Hammer; Goeh Jung ABSTRACT Myosins I are ubiquitous, nonfilamentous, actin-based mechanoenzymes originally discovered in protozoa. The extensive in vitro biochemical studies of purified protozoan myosins I are now being complemented with in vivo studies using cloned myosin I heavy chain...