... the expression and function of TCF7L2 (also known as TCF4), using several models of MSCs, including skeletal muscle fibro-adipogenic progenitors. We show that TCF/LEFs are differentially expressed and that TGF-β reduces the expression of TCF7L2 in MSCs but not in myoblasts. We also found that the ubiquitin...

... activity is associated with increased viability of human myoblasts. In addition to its detoxifying activity, aldehyde dehydrogenase can also catalyze the irreversible oxidation of vitamin A to retinoic acid; therefore, we examined whether retinoic acid is important for myoblast viability. We showed...

... © 2012. 2012 Slow-dividing cells Satellite cells Skeletal muscle Myoblasts Self-renewal Stemness Satellite cells are muscle tissue stem cells located between the basal lamina and plasma lemma of myofibres and play important roles in postnatal muscle growth, as well as adult muscle...

... myoblasts sensitized the cells to mitochondria-associated intrinsic apoptosis induced by cell confluence or serum starvation. Further investigation of this pathway revealed that M-cadherin-mediated signaling suppressed GSK-3β activation by enhancing the PI3K/AKT-dependent inhibitory phosphorylation of Ser9...

... on proliferation, migration and myogenic differentiation of mouse C2C12 myogenic progenitor/myoblast cells. In addition, we have characterized the signaling pathways employed by SDF1-CXCR4 to control myogenesis. We found that SDF1 stimulates proliferation and induces migration of C2C12 cells with a potency similar...

...Nathan T. Swailes; Melanie Colegrave; Peter J. Knight; Michelle Peckham The interaction of non-muscle myosins 2A and 2B with actin may drive changes in cell movement, shape and adhesion. To investigate this, we used cultured myoblasts as a model system. These cells characteristically change shape...

... and regenerating tibialis anterior muscle and in primary cultures of differentiating myoblasts in adult and newborn mice, respectively. Cxs 45 and 43, but not 40, were strongly expressed in normal muscle and their expression was upregulated during regeneration. Furthermore, the functional role of Cx43 during...

... stem cells Myoblasts Adipocytes Pax7 protein The growth and repair potential of adult skeletal muscle relies on a reservoir of myogenic precursors termed satellite cells that reside at the periphery of myofibers beneath their basement membrane ( Mauro, 1961 ). In a mature muscle, satellite...

... to this lectin results in 100% conversion of the cells. Conversion was assessed by the expression within the cells of the muscle-specific cytoskeletal protein desmin. We also investigate the effects of galectin-1 on cells of the C2C12 mouse myogenic cell line and on primary mouse myoblasts. Exposing both...

... lamin speckles that associate with RNA splicing factor speckles in C2C12 myoblasts and myotubes. Lamin speckles were observed in dividing myoblasts but disappeared early during the course of differentiation in postmitotic myocytes, and were absent in myotubes and muscle fibers. However, no changes were...

... used to generate isoform-specific monoclonal antibodies. Expression of the two isoforms during myogenesis was followed in the myogenic cell lines BC 3 H1 and L6. Only the 47 kDa isoform was present in undifferentiated L6 or BC 3 H1 myoblasts. The 51 kDa isoform appeared after myogenesis had been...

... cadmium concentrations down to 100 pM stimulate cell growth and DNA synthesis significantly. Stimulation is found in all three mammalian cell types examined: namely L6J1, a rat permanent myoblast cell line, LLC-PK 1 porcine renal epithelial cells, and a primary rat chondrocyte culture. Cd acts...

... and of microfilament polymerisation all prevented perpendicular elongation. A model involving strengthened adhesion asymmetrically along the cathodal-facing side of round myoblasts and incorporating the above requirements is proposed to explain electric field-induced perpendicular differentiation. Some asymmetry...

...Nansi Jo Colley; K. T. Tokuyasu; S. J. Singer ABSTRACT Previous reports on skeletal muscle myogenesis have shown that postmitotic spindle-shaped myoblasts express muscle-specific proteins, some of which are organized into nascent myofibrils. However, we show that, in skeletal muscle cultures...