...Margaret Hung; Hsiao-Fan Lo; Grace E. L. Jones; Robert S. Krauss ABSTRACT Skeletal muscle stem cells (MuSCs, also called satellite cells) are the source of the robust regenerative capability of this tissue. The hallmark property of MuSCs at homeostasis is quiescence, a reversible state of cell...

...Nicanor González Morales; Océane Marescal; Szilárd Szikora; Anja Katzemich; Tuana Correia-Mesquita; Péter Bíró; Miklos Erdelyi; József Mihály; Frieder Schöck ABSTRACT Myofibrils are long intracellular cables specific to muscles, composed mainly of actin and myosin filaments. The actin and myosin...

... data are available from precise genetic manipulations. All these data aim to answer how the Z-disc works and how it is assembled. Here, we summarize recent data from insects and explore how it fits into our view of the Z-disc, myofibrils and, ultimately, muscles. The complex arrangement between actin...

... and behaviour are fundamentally important for understanding how disease, molecules and drugs affect cell function in vivo . Here, by using muscle stem cell (muSC) responses to injury in zebrafish as our biological paradigm, we established a ‘ground truth’ for muSC behaviour. This revealed that segmentation...

... the mechanisms or significance that underlies distinct lysosomal morphologies. Here, we found an extensive tubular autolysosomal network in Drosophila abdominal muscle remodeling during metamorphosis. The tubular network transiently appeared and exhibited the capacity to degrade autophagic cargoes. The tubular...

... of the β1 integrin cytoplasmic tail creates ubiquitously expressed β1A, and the heart and skeletal muscle-specific β1D form. To study the physiological difference between these forms, we developed fluorescent β1 integrins and analyzed their dynamics, localization, and cytoplasmic adapter recruitment...

... responses that are activated following the induction of muscle stresses, including hypercontraction, detachment and oxidative stress. Of these stressors, loss of the genes that cause muscle detachment produced the strongest levels of JAK-STAT activation. In one of these mutants, fondue ( fon ), we also...

...Benjamin Richier; Yoshiko Inoue; Ulrich Dobramysl; Jonathan Friedlander; Nicholas H. Brown; Jennifer L. Gallop ABSTRACT Cells need to sense their environment to ensure accurate targeting to specific destinations. This occurs in developing muscles, which need to attach to tendon cells before muscle...

...Maik Drechsler; Heiko Meyer; Ariane C. Wilmes; Achim Paululat ABSTRACT Somatic muscles are formed by the iterative fusion of myoblasts into muscle fibres. This process is driven by the recurrent recruitment of proteins to the cell membrane to induce F-actin nucleation at the fusion site. Although...

... of Atad3 (also known as Atad3a ) was embryonic lethal, but a skeletal muscle-specific conditional knockout (KO) was viable. At birth, ATAD3 muscle KO mice had normal weight, but from 2 months onwards they showed progressive motor-impaired coordination and weakness. Loss of ATAD3 caused early and severe...

...Alexander L. Auld; Sacha A. Roberts; Ciaran B. Murphy; Jaclyn M. Camuglia; Eric S. Folker ABSTRACT During muscle development, myonuclei undergo a complex set of movements that result in evenly spaced nuclei throughout the muscle cell. In Drosophila , two separate pools of Kinesin and Dynein work...

..., we show that these effects are also reflected at the organism level, as there is a loss of skeletal muscle mass in nestin −/− (NesKO) mice, reflected as reduced lean (muscle) mass in the mice. Further examination of muscles in male mice revealed that these effects stemmed from nestin-deficient...

... damage. Genes involved in muscle growth and development were upregulated, but those associated with responses to cellular stresses were downregulated. These data suggest that elimination of HIRA produces a hypertrophic response in skeletal muscle and leaves myofibers susceptible to stress-induced...

... is largely unknown. We show that the conserved transmembrane Drosophila proteoglycan Kon-tiki (Kon, also known as Perdido) interacts with the αPS2βPS integrin (αPS2 is encoded by inflated and βPS by myospheroid ) to mediate muscle–tendon adhesion. kon and inflated double mutant embryos show a synergistic...

...Wei Wei; Wei-Ya Zhang; Jian-Bo Bai; Hai-Xin Zhang; Yuan-Yuan Zhao; Xin-Yun Li; Shu-Hong Zhao ABSTRACT MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development...

...Juan J. Pérez-Moreno; Marcus Bischoff; Maria D. Martín-Bermudo; Beatriz Estrada ABSTRACT Muscle differentiation requires the assembly of high-order structures called myofibrils, composed of sarcomeres. Even though the molecular organization of sarcomeres is well known, the mechanisms underlying...

...Ze Cindy Liu; Nadia Odell; Erika R. Geisbrecht Summary Establishment and maintenance of stable muscle attachments is essential for coordinated body movement. Studies in Drosophila have pioneered a molecular understanding of the morphological events in the conserved process of muscle attachment...

... element. In this first experimental analysis of function, we show that transgenic expression of each of the three family members in skeletal muscle causes significant fiber type shifts, but the GTF2IRD2 protein causes an extreme shift in the opposite direction to the two other family members. Mating...

...Kazumi Nomura; Kanako Ono; Shoichiro Ono Summary Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril...

...Meredith H. Wilson; Erika L. F. Holzbaur Summary Dynamic interactions with the cytoskeleton drive the movement and positioning of nuclei in many cell types. During muscle cell development, myoblasts fuse to form syncytial myofibers with nuclei positioned regularly along the length of the cell...