... inhibitor treatments. Chemical biology Chromatin Epigenetics Histone modification Monoclonal antibody Japan Society for the Promotion of Science 10.13039/501100001691 JP25116005 JP26291071 JP17H01417 JP18H05527 JP21404010 JP25560408 JP26221204 JP18H02100 Japan Science...

... a conformation-dependent anti-α 5 monoclonal antibody (SNAKA51) that converts α 5 β 1 integrin into a ligand-competent form and promotes fibronectin binding. In adherent fibroblasts, SNAKA51 preferentially bound to integrins in fibrillar adhesions. Clustering of integrins expressing this activation epitope...

... in their natural environment and the effects of monoclonal antibodies specific for α and β chains. Cells driven by a force too low to alter the natural lifetime of a single bond displayed multiple arrests. Studying the frequency and duration of these arrests yielded fairly direct information on the rate of bond...

... to TLS into the cytoplasm of cycloheximide treated HeLa cells and examined the effect this would have on the distribution of the protein. Cells injected with a monoclonal antibody to the C terminus of TLS (4H11) were fixed 2 hours later and stained with rabbit anti-N-terminal TLS. The injected cells were...

... Coiled coil protein Immunofluorescence Monoclonal antibody Matrix targeting sequence HMP Mitochondria are dynamic organelles which replicate semi-autonomously, bifurcate and re-fuse, and are actively transported through the cytoplasmic volume (reviewed by Attardi and Schatz, 1988 ; Bereiter...

... laminin-5r, perhaps during matrix assembly, and that the 5C5 epitope may serve as a marker for assembled laminin-5r matrix. * Author for correspondence 12 01 1996 03 04 1996 © 1996 by Company of Biologists 1996 Extracellular matrix Laminin Monoclonal antibody...

...-growing pollen tubes. These rapidly growing cells are highly secretory and require a high level of plasma membrane recycling to maintain the tube tip architecture. Monoclonal antibodies to plant clathrin HC confirmed that coated vesicles are rela tively abundant in tip-growing pollen tubes of Lilium...

...Marie-Hélène Bré; Virginie Redeker; Martin Quibell; Julienne Darmanaden-Delorme; Christophe Bressac; Jacky Cosson; Philippe Huitorel; Jean-Marie Schmitter; Jean Rossier; Tony Johnson; André Adoutte; Nicolette Levilliers ABSTRACT Two monoclonal antibodies, AXO 49 and TAP 952, probed with carboxy...

...Shogo Nakamura; Genta Tanaka; Tsukasa Maeda; Ritsu Kamiya; Tsukasa Matsunaga; Osamu Nikaido ABSTRACT Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii , and found...

... fragments. Monoclonal antibody MAb17, which binds to the cell binding region of fibronectin, partially inhibited the attachment of both human bone cells and corneal epithelial cells to intact fibronectin when present at 50 µg/ml and reduced human bone cell attachment to the cell-binding region fragment...

...Pascale Dozolme; Danièle Marty-Mazars; Marie-Claude Clémencet; Francis Marty ABSTRACT A monoclonal antibody, designated TeM 106, that recognizes an intrinsic protein from the vacuole membrane (tonoplast) of cauliflower (Brassica oleracea L. var. botrytis) is described. Mice were immunized...

...J. Rodriguez; V. Boulo; E. Mialhe; E. Bachere ABSTRACT Various haemolymph components of the shrimp Penaeus japonicus were identified and characterised by monoclonal antibodies. Three groups of monoclonal antibodies were raised. Their reactivity to haemocyte types and/or secreted molecules...

... the dynamics of membrane retrieval in the phagosome system of Paramecium multimicronucleatum using six monoclonal antibody markers. Our previous freeze-fracture and transmission electron microscopic studies have indicated that extensive changes take place in the membrane of the young phagosome as it progresses...

... plays a role in sperm-oocyte fusion. Monoclonal antibodies were raised against unfixed acrosome-reacted guinea pig sperm and screened by indirect immunofluorescence for binding to the equatorial segment. They were back-screened against unfixed acrosome-intact sperm for absence of binding. Using...

... the presence and behavior of certain components associated with centrosomes of the surf clam Spisula solidissima and cultured mammalian cells. A Sarkosyl detergent-resistant fraction of axonemal microtubules was isolated from sea urchin sperm flagella and used to produce monoclonal antibodies, 16 of which were...

...Agnes K. Fok; Margaret Clarke; Le Ma; Richard D. Allen ABSTRACT A Dictyostelium membrane fraction rich in vacuolar proton pumps, previously described by Nolta et al. (J. Biol. Chem . 266, 18,318-18,323, 1991), was used as the immunogen for production of monoclonal antibodies. We obtained antibodies...

...Yoshinao Kaneda; Katsunari Kinoshita; Manabu Sato; Kiyoji Tanaka; Yasufumi Kaneda ABSTRACT We previously reported that the monoclonal antibody M108 recognized a 40 kDa protein both in the nucleus and the cytoplasm. This nuclear 40 kDa antigen was located in the nuclear envelope in interphase cells...

...Masaki Ishida; Marilynn S. Aihara; Richard D. Allen; Agnes K. Fok ABSTRACT In a previous study, monoclonal antibody DS-1 was found to specifically label the decorated spongiome along the radial arms of the contractile vacuole complexes in Paramecium multimicronucleatum . Fluorescein isothiocyanate...

... membrane networks; it constitutes the predominant structure in Paramecium cell ghosts. Previous indirect data suggested several cortical proteins as potential constituents of the epiplasm. New sharp monoclonal antibodies presented in this paper, positive both on immunotransfers and in immunocytochemical...

.... 1992 Golgi apparatus monoclonal antibody JIM 84 Brefeldin A vesicle traffic endomembranes exocytosis The plant cell secretory process is a result of sequential activity along a vesicle-mediated pathway from the endoplasmic reticulum (ER) to the cell surface (reviewed by Hawes et...