...Ana C. Almeida; Helder Rocha; Maximilian W. D. Raas; Hanh Witte; Ralf J. Sommer; Berend Snel; Geert J. P. L. Kops; Reto Gassmann; Helder Maiato ABSTRACT Chromosome alignment during mitosis can occur as a consequence of bi-orientation or is assisted by the CENP-E (kinesin-7) motor at kinetochores...

...Laura Bel Borja; Samuel J. P. Taylor; Flavie Soubigou; Federico Pelisch ABSTRACT Polo-like kinase 1 (PLK-1) is present in centrosomes, the nuclear envelope and kinetochores and plays a significant role in meiosis and mitosis. PLK-1 depletion or inhibition has severe consequences for spindle...

... crucial roles of the ER in mitosis. During mitosis, the ER is remodelled significantly with respect to composition and morphology but persists as a continuous network. The ER interacts with microtubules, actin and intermediate filaments, and concomitant with the mitotic restructuring of all cytoskeletal...

... mitosis, by accumulation of a cytoskeletal structure called the preprophase band (PPB). The PPB is thought to be essential for recruitment of division site-localized proteins, which remain at the division site after the PPB disassembles. Here, we show that the division site-localized protein TANGLED1...

... in these events. However, mechanistical understanding of mitosis and cytokinesis in dinoflagellates remains rudimentary. Using an optimized immunofluorescence protocol, we analysed changes in microtubule organization occurring during the mitotic cycle of the toxic dinoflagellate Ostreopsis cf. ovata. We find...

... and to the centrosome, spindle midzone and bridging fiber area, and midbodies during mitosis. Loss of DPF3 causes kinetochore fiber instability, unstable kinetochore–microtubule attachment and defects in chromosome alignment, resulting in altered mitotic progression, cell death and genomic instability. In addition, we...

... cohesion establishment and mitotic cohesion dissolution have been extensively explored, the regulation of cohesin loading is still poorly understood. Here, we report that the methyltransferase NSD3 is essential for mitotic sister chromatid cohesion before mitosis entry. NSD3 interacts with the cohesin...

... both with and without cell cycle synchronization. We found that single-cell pHi is dynamic throughout the cell cycle: pHi decreases at G1/S, increases in mid-S, decreases at late S, increases at G2/M and rapidly decreases during mitosis. Importantly, although pHi is highly dynamic in dividing cells...

... localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role...

... previously reported that protocadherin 7 (PCDH7) gets enriched at the cell surface during mitosis, which is required to build up the full mitotic rounding pressure. Here, we report that PCDH7 interacts with and is palmitoylated by the palmitoyltransferase, ZDHHC5. PCDH7 and ZDHHC5 colocalize at the mitotic...

... Gable , A. , Qiu , M. , Titus , J. , Balchand , S. , Ferenz , N. P. , Ma , N. , Collins , E. S. , Fagerstrom , C. , Ross , J. L. , Yang , G. et al. ( 2012 ). Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2...

...Carolyn M. Remsburg; Kalin D. Konrad; Jia L. Song ABSTRACT Mitosis is a fundamental and highly regulated process that acts to faithfully segregate chromosomes into two identical daughter cells. Localization of gene transcripts involved in mitosis to the mitotic spindle might be an evolutionarily...

...: The Review summarizes current knowledge about how dynein at the kinetochore contributes to efficient and accurate spindle assembly and describes the underlying molecular mechanisms. Dynein Corona Kinetochore Mitosis Spindly RZZ Nde1 Ndel1 Lis1 CENP-F Fundação para a Ciência e...

...-regulating sequences, correlate with colorectal cancer. The cellular roles of APC in mitosis are widely studied, but the molecular mechanisms of its interaction with the cytoskeleton are poorly understood. Here, we investigated how APC-C regulates microtubule properties, and found that it promotes both...

.... Published by The Company of Biologists Ltd 2023 Summary: Mhf2, a component of the inner layer of the proteinaceous kinetochore supercomplex, regulates the spindle assembly checkpoint and faithful chromosome segregation. Fission yeast Kinetochore Mhf2 Mitosis Spindle assembly checkpoint...

.... Kinetochore Mitosis Lis1 Dynein Microtubule King's College London Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BB/T008709/1 Accurate mitotic progression requires that chromosomes capture spindle microtubules and form...

... meristematic zone rescues cell-file-rotation defects in tan1 air9 mutant plants, suggesting that defects that occur in mitosis influence the organization of non-dividing cells. Mitosis Phragmoplast Root twisting Cell file rotation Division plane orientation Arabidopsis National Science...

...Kehan Xu; Chunxue Wang; Kari Keinänen; Hong Li; Chunlin Cai ABSTRACT Swift and complete spindle disassembly in late mitosis is essential for cell survival, yet how it happens is largely unknown in mammalian cells. Here we used real-time live cell microscopy and biochemical assays to show...

... via micronuclei formation. Chromosomal instability Aneuploidy Micronuclei KIF11 Mitosis CRISPR activation Confocal imaging Machine learning Deep learning Cancer Research UK http://dx.doi.org/10.13039/501100000289 C416/A25196 CRUK Lung Cancer Centre of Excellence...

...Souradeep Basu; James O. Patterson; Theresa U. Zeisner; Paul Nurse ABSTRACT The eukaryotic cell cycle is driven by the activity of cyclin-dependent kinases (CDKs). CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. However, it is not known...