.... In the absence of Drak2 , the amount of polymerized actin was decreased , suggesting that DRAK2 modulates actomyosin dynamics. We further show that myosin-dependent T cell functions, such as migration, T cell receptor microcluster accumulation, and conjugation to antigen presenting cells are decreased...

... parasite, adhesion to host cells is essential for the development of infection. During attachment, the parasite changes its tear ovoid shape to a flat ameboid form, expanding the contact surface and migrating through tissues. Here, we have identified a novel structure formed at the posterior pole...

... a chemorepellent. Dictyostelium Chemotaxis Chemorepulsion Cytoskeleton Migration Phosphoinositide Phosphatidylinositol PTEN National Institutes of Health http://dx.doi.org/10.13039/100000002 GM118355 GM139486 Texas A&M University http://dx.doi.org/10.13039/100007904...

... vascular stability. The spatiotemporal activation of the transcriptional co-factors YAP (herein referring to YAP1) and TAZ (also known WWTR1), collectively denoted YAP/TAZ, is crucial to allow for efficient collective endothelial migration in angiogenesis. The focal adhesion protein deleted-in-liver-cancer...

...: Quantitative dynamic analysis of neuronal growth cone filopodia formation reveals a density-dependent interaction between the membrane adaptor TOCA-1 and actin filament elongating protein Ena. Growth cone Actin Migration References Applewhite , D. A. , Barzik , M. , Kojima , S...

... in ARPC1B and show impaired migration and a switch from forming pseudopodia to forming elongated filopodia. We show, using a blood vessel on a chip model, that primary human neutrophils have impaired movement across an endothelium deficient in APRC1B. We also show that the combined deficiency of ARPC1B...

... been postulated that the unique segmented nucleus of neutrophils functions to assist their rapid migration. Here, we tested this hypothesis by imaging primary human neutrophils traversing narrow channels in custom-designed microfluidic devices. Individuals were given an intravenous low dose...

... precursors and defective formation of growth cones in young neurons. Here, we present new evidence that upon Rac1 and Rac3 ablation, centrosome, Golgi complex and lysosome positioning is significantly perturbed, thus affecting both interneuron migration and axon growth. Moreover, for the first time, we...

... focal adhesion disassembly. LL5β is phosphorylated by MINK1, promoting its interaction with CLASP proteins. Using a kinase inhibitor, we demonstrate that the enzymatic activity of MINK1 is involved in PRICKLE1–LL5β complex assembly and localization, as well as in cell migration. Analysis of gene...

... to be recycled back to the cell membrane. We show that PDGFRβ homodimer activation results in a greater amplitude of phospho-ERK1/2 and phospho-AKT signaling, as well as increased proliferation and migration. Finally, we demonstrate that inhibition of clathrin-mediated endocytosis leads to changes in cellular...

... characterized, particularly during development . Although exosomes released by invasive metastatic cells alter migration of neighboring cells in culture, it is unclear whether cancer cells misappropriate exosomes released by healthy differentiated cells or reactivate dormant developmental programs that include...

...Kristiane Søreng; Serhiy Pankiv; Camilla Bergsmark; Ellen M. Haugsten; Anette K. Dahl; Laura R. de la Ballina; Ai Yamamoto; Alf H. Lystad; Anne Simonsen ABSTRACT Cell migration is a complex process underlying physiological and pathological processes such as brain development and cancer metastasis...

... was maintained despite cells readily delaminating from epithelial sheets. Distinct from the prominent filopodia in p110α H1047R -expressing cells, p110β induced formation of lamellipodia, and these cells migrated with significantly greater velocity and decreased directionality. p110β-induced phenotypic...

... oligomers and key features of SRC signal encoding and decision-making activity. SRC Conformational intermediates Optogenetics Time-resolved phosphoproteomics Pleiotropy Migration Invasion Encoding intracellular signaling Cytoskeletal Adhesions Agence Nationale de la Recherche http...

...Nathan H. Roy; Sarah Hyun Ji Kim; Alexander Buffone, Jr; Daniel Blumenthal; Bonnie Huang; Sangya Agarwal; Pamela L. Schwartzberg; Daniel A. Hammer; Janis K. Burkhardt ABSTRACT T cell entry into inflamed tissue requires firm adhesion, cell spreading, and migration along and through the endothelial...

... and the transmembrane heparan sulfate proteoglycan syndecan-4 in influencing adhesion, migration and angiogenesis. Knockdown of ADAMTS-1 in endothelial cells resulted in a parallel reduction in cell surface syndecan-4, attributable to increased matrix metalloproteinase-9 (MMP9) activity. Knockdown of either ADAMTS-1...

...Daniel A. Girón-Pérez; Eduardo Vadillo; Michael Schnoor; Leopoldo Santos-Argumedo; Ana-Maria Lennon-Duménil ABSTRACT The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin...

...-polarized hMDMs. Using PTPε-specific siRNAs and expression of RPTPε and cyt-PTPε, we found that RPTPε is involved in monocyte adhesion and migration of M2-polarized hMDMs in response to LTD 4 . Altered organization of podosomes and higher phosphorylation of the inhibitory Y-722 residue of ROCK2 was also...

...Kim Kenwrick; Amrita Mukherjee; Andrew D. Renault ABSTRACT During development, many cell types migrate along stereotyped routes determined through deployment of cell surface or secreted guidance molecules. Although we know the identity of many of these molecules, the distances over which...

... and resulted in a thicker epidermal layer after reepithelialization. Conversely, preventing Cx43 phosphorylation by mitogen-activated protein kinases (MAPKs) through mutation significantly increased the rate of wound closure in vivo . Defects in migration, but not proliferation, in all mutants were partially...