...Helena Pissarek; Na Huang; Leanna H. Frasch; Hermann Aberle; Manfred Frasch The cytoskeleton of Drosophila tendon cells features specialized F-actin/microtubule arrays that endow these cells with resistance to the tensile forces exerted by the attached muscles. In a forward genetic screen...

...Daisy Duan; Anthony J. Koleske ABSTRACT Protein liquid–liquid phase separation (LLPS) is driven by intrinsically disordered regions and multivalent binding domains, both of which are common features of diverse microtubule (MT) regulators. Many in vitro studies have dissected the mechanisms by which...

... snapshots of various mitotic stages provided a comprehensive map of nucleolar and mitochondrial segregation dynamics and enabled the resolution of the inner and outer plaque of spindle pole bodies (SPBs). Analyses of microtubules (MTs) and SPBs suggest that C. albicans displays a side-by-side SPB...

... cytoskeleton. We now describe a new function for the Chlamydia pneumoniae TarP member CPn0572, namely the ability to bind and alter the microtubule cytoskeleton. Thus, CPn0572 is unique in being the only prokaryotic protein that directly modulates both dynamic cytoskeletons of a eukaryotic cell. Ectopically...

..., and the underlying longitudinal microtubule array has a key role during tube budding of salivary glands in the Drosophila embryo. At this microtubule–actomyosin interface, a hub of proteins accumulates, and we have shown before that this hub includes the microtubule–actin crosslinker Shot and the microtubule minus...

... inhibition reduced axon extension without significantly altering initial outgrowth. In addition, RhoA–mDia signaling promoted axon elongation by stimulating growth cone microtubule stability and assembly, as opposed to RhoA–ROCK signaling, which restrained growth cone microtubule assembly and protrusion...

...Shweta Shyam Prassanawar; Tuhin Sarkar; Dulal Panda ABSTRACT Centrosomal proteins play pivotal roles in orchestrating microtubule dynamics, and their dysregulation leads to disorders, including cancer and ciliopathies. Understanding the multifaceted roles of centrosomal proteins is vital...

... in these events. However, mechanistical understanding of mitosis and cytokinesis in dinoflagellates remains rudimentary. Using an optimized immunofluorescence protocol, we analysed changes in microtubule organization occurring during the mitotic cycle of the toxic dinoflagellate Ostreopsis cf. ovata. We find...

...Amrita Mukherjee; Yaiza Andrés Jeske; Isabelle Becam; Anaelle Taïeb; Paul Brooks; Joanna Aouad; Clementine Monguillon; Paul T. Conduit ABSTRACT Microtubules are nucleated by γ-tubulin ring complexes (γ-TuRCs) and are essential for neuronal development. Nevertheless, γ-TuRC depletion has been...

...-specific morphology, which relies on dynamic support by microtubules (MTs). An important player is the augmin complex (hereafter augmin), which binds to existing MT filaments and recruits the γ-tubulin ring complex (γ-TuRC), to form branched MTs. In cultured neurons, augmin is important for neurite...

... involved in the regulation of microtubule dynamics and function in eukaryotes. In the protozoan parasite Trypanosoma brucei , the microtubule cytoskeleton is essential for cell motility and maintaining cell shape. In a previous study, we showed that T. brucei TTLL6A and TTLL12B are required to regulate...

...Hannah L. Glover; Marta Mendes; Joana Gomes-Neto; Emma V. Rusilowicz-Jones; Daniel J. Rigden; Gunnar Dittmar; Sylvie Urbé; Michael J. Clague ABSTRACT The microtubule network is formed from polymerised tubulin subunits and associating proteins, which govern microtubule dynamics and a diverse array...

... and secretory domain at the interface with their target, the immune synapse (IS). During IS formation, CTLs acquire a transient polarity, marked by re-orientation of the centrosome and microtubule cytoskeleton toward the IS, thus directing the transport and delivery of the lytic granules to the target cell...

...Jovana Deretic; Ezgi Odabasi; Elif Nur Firat-Karalar ABSTRACT The primary cilium is a conserved microtubule-based organelle that is critical for transducing developmental, sensory and homeostatic signaling pathways. It comprises an axoneme with nine parallel doublet microtubules extending from...

...Zane J. Bergman; Jonathan J. Wong; David G. Drubin; Georjana Barnes ABSTRACT The budding yeast Saccharomyces cerevisiae has a closed mitosis in which the mitotic spindle and the cytoplasmic microtubules (MTs), both of which generate forces to faithfully segregate chromosomes, remain separated...

...Arielle Flinois; Isabelle Méan; Annick Mutero-Maeda; Laurent Guillemot; Sandra Citi ABSTRACT Paracingulin (CGNL1) is recruited to tight junctions (TJs) by ZO-1 and to adherens junctions (AJs) by PLEKHA7. PLEKHA7 has been reported to bind to the microtubule minus-end-binding protein CAMSAP3...

...Leeba Ann Chacko; Felix Mikus; Nicholas Ariotti; Gautam Dey; Vaishnavi Ananthanarayanan ABSTRACT Association with microtubules inhibits the fission of mitochondria in Schizosaccharomyces pombe . Here, we show that this attachment of mitochondria to microtubules is an important cell-intrinsic factor...

... demonstrate that U-ExM allows imaging of the microtubule cytoskeleton and its associated spindle pole body, notably unveiling the Sfi1p–Cdc31p spatial organization on the appendage bridge structure. In S. pombe , we validate the method by monitoring the homeostatic regulation of nuclear pore complex number...

... endosomal choreography, which includes bidirectional and stop-and-go motions, is coordinated by the microtubule-based motor proteins dynein and kinesin. These motors bridge various endosomal subtypes to the microtubule tracks thanks to their cargo-binding domain interacting with endosome-associated proteins...

..., for intracellular transport. Here we describe how molecular motors and cytoskeletal tracks can generate streaming. Actin Cytoplasmic streaming Dynein Kinesin Microtubules Myosin National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35 GM131752...